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[187954_s_at]

Description F52D10.3 /REP_DB=WormBase Gene ID /WP=CE03389 /CHR=X /FEA=Sanger Annotation /DEF=locus:ftt-2 14-3-3 protein (HINXTON) SW:Q20655
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 04      4grp_highest_8_8 > 40      4grp_high_8_8 > 50      6grp_highest_nonorm > 06      6grp_total > 24      4grp_total > 61      6grp_highest_sub > De      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4619
1 2 3 4 range
A 13183.3
(2.5)
P 		13428
(3)
P 		13107.1
(2.4)
P 		10187.6
(-3)
P 		3240
B 14807
(3)
P 		12270.9
(-1.8)
P 		12816.7
(-0.7)
P 		11615
(-3)
P 		3192
C 12327.3
(3)
P 		12319.8
(3)
P 		11148.4
(-1.4)
P 		10709.2
(-3)
P 		1618
avr 13439.2
(3) 		12672.9
(1.2) 		12357.4
(0.5) 		10837.3
(-3) 		2602

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5991
1 2 3 4 5 6 range
A 12571.9
(3)
P 		10278.5
(-0.3)
P 		8998.2
(-2.1)
P 		8510.2
(-2.8)
P 		8492.7
(-2.8)
P 		8338.4
(-3)
P 		4234
B 10694.3
(2.8)
P 		10794.8
(3)
P 		10787
(3)
P 		8841.3
(0)
P 		7977.6
(-1.3)
P 		6828.1
(-3)
P 		3967
C 11077.9
(2.8)
P 		11248.4
(3)
P 		10578.9
(2.1)
P 		8208.9
(-0.9)
P 		8565.6
(-0.4)
P 		6581.3
(-3)
P 		4667
avr 11448
(3) 		10773.9
(2) 		10121.4
(1.1) 		8520.1
(-1.2) 		8345.3
(-1.4) 		7249.3
(-3) 		4199
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3309
1 2 3 4 range
A 4800
(3)
P 		2914.9
(-0.4)
P 		3426.4
(0.5)
P 		1491.3
(-3)
P 		3309
B 4721.8
(3)
P 		3030.6
(-1.5)
P 		2969.9
(-1.7)
P 		2475.2
(-3)
P 		2247
C 4080.7
(3)
P 		3542
(1.6)
P 		3885.6
(2.5)
P 		1805.7
(-3)
P 		2275
avr 4534.2
(3) 		3162.5
(-0.2) 		3427.3
(0.5) 		1924.1
(-3) 		2610

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2455
1 2 3 4 5 6 range
A 2317.5
(-1.7)
P 		3655.5
(3)
P 		1959.6
(-3)
P 		2158.4
(-2.3)
P 		2562.7
(-0.9)
P 		2030.1
(-2.8)
P 		1696
B 3721.1
(3)
P 		3593.1
(2.4)
P 		2619.4
(-1.8)
P 		2341.8
(-3)
P 		3241.2
(0.9)
P 		2546.2
(-2.1)
P 		1379
C 4106.1
(3)
P 		3432.2
(1.4)
P 		2692.5
(-0.5)
P 		1650.8
(-3)
P 		2771.7
(-0.3)
P 		2203.1
(-1.7)
P 		2455
avr 3381.6
(2.3) 		3560.3
(3) 		2423.8
(-1.5) 		2050.3
(-3) 		2858.5
(0.2) 		2259.8
(-2.2) 		1510
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187954_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16587
Sequence Type Exemplar sequence
Representative Public ID F52D10.3 NCBI
Target Description F52D10.3 /REP_DB=WormBase Gene ID /WP=CE03389 /CHR=X /FEA=Sanger Annotation /DEF=locus:ftt-2 14-3-3 protein (HINXTON) SW:Q20655
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077538(11), NM_077537(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077538 NCBI Caenorhabditis elegans Fourteen-Three-Three family member (ftt-2) (ftt-2) mRNA, complete cds. 11/11 None
NM_077537 NCBI Caenorhabditis elegans Fourteen-Three-Three family member (ftt-2) (ftt-2) mRNA, complete cds. 10/11 None
SNAP00000034608 ENSEMBL cdna:SNAP chromosome:CEL140:X:11603974:11605301:-1 10/11 None
GENEFINDER00000034617 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:11603974:11606480:-1 11/11 None
F52D10.3a.1 ENSEMBL cdna:known chromosome:CEL140:X:11603632:11605303:-1 gene:F52D10.3 11/11 A 
F52D10.3b.1 ENSEMBL cdna:known chromosome:CEL140:X:11603635:11605333:-1 gene:F52D10.3 10/11 A 
F52D10.3b.2 ENSEMBL cdna:known chromosome:CEL140:X:11603635:11605342:-1 gene:F52D10.3 10/11 A 
F52D10.3b.3 ENSEMBL cdna:known chromosome:CEL140:X:11603635:11605690:-1 gene:F52D10.3 10/11 A 
F52D10.3a.2 ENSEMBL cdna:known chromosome:CEL140:X:11603654:11605303:-1 gene:F52D10.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
F52D10.3b.4 7/11 Cross Hyb Matching Probes None
F52D10.3b.5 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:11603973-11605301(-) 100.0 100.0
Public Domain and Genome References
Ensembl F52D10.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91474 Hypothetical protein F52D10.3a [Caenorhabditis elegans] ref|NP_509939.1| Fourteen-Three-Three family member (ftt-2) [Caenorhabditis elegans] sp|Q20655|14332_CAEEL 14-3-3-like protein 1.0E-129
blast CAE70609 Hypothetical protein CBG17289 [Caenorhabditis briggsae] 1.0E-128
blast AAR85527 14-3-3b protein [Meloidogyne incognita] 1.0E-118
Protein Domains
Database ID Description E-Value
Pfam IPR000308 EMBL-EBI 14-3-3 protein 1.0E-126
Pfam IPR000308 EMBL-EBI 14-3-3 protein 2.0E-115
Pfam IPR000308 EMBL-EBI 14-3-3 protein 1.8E-51
Pfam IPR000308 EMBL-EBI 14-3-3 protein 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:187954_S_AT
aacgaggagcgcaatcttctctccgttgcctacaagaacgtcgtcggagcccgtcgttct
tcatggagagtcatctcttcaattgagcagaagaccgaaggatctgagaagaagcaacaa
atggccaaggaataccgtgagaaggttgagaaggagctccgcgacatctgccaagacgtt
ctcaacttgctcgacaagttcctcattccaaaggctggagccgccgagtccaaggtcttc
tacctcaagatgaagggagactactaccgttacctcgctgaggtcgcctccggagacgac
agaaactcggttgtcgagaagtcgcagcaaagctaccaagaggcgttcgacattgccaag
gataagatgcaaccaactcacccaatccgcctgggacttgctctcaacttctctgtcttc
ttctatgagatcttgaacgccccggacaaggcttgccagcttgccaagcaggctttcgat
gacgccatcgctgagctcgacaccctcaacgaggactcatacaaggactctaccctcatc
atgcagcttctccgtgacaacttgactctctggac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACGAGGAGCGCAATCTTCTCTCCG 626 143 130 Antisense
CTTCTCTCCGTTGCCTACAAGAACG 167 221 145 Antisense
ACATCTGCCAAGACGTTCTCAACTT 480 105 293 Antisense
GACAAGTTCCTCATTCCAAAGGCTG 344 365 322 Antisense
AGTCCAAGGTCTTCTACCTCAAGAT 473 57 356 Antisense
GGTCGCCTCCGGAGACGACAGAAAC 165 499 411 Antisense
TTCTATGAGATCTTGAACGCCCCGG 334 689 550 Antisense
GCCAAGCAGGCTTTCGATGACGCCA 590 277 592 Antisense
CTCGACACCCTCAACGAGGACTCAT 52 233 625 Antisense
TCATACAAGGACTCTACCCTCATCA 15 621 646 Antisense
TCCGTGACAACTTGACTCTCTGGAC 428 595 680 Antisense
Sequence Source Affymetrix Proprietary Database