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[187953_at] channel protein

Description M60.2_rc /REP_DB=WormBase Gene ID /WP=CE04775 /TR=Q9GYM9 /GB=AAF99996.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /REVCOMP
Cluster 6grp_high1_10_10 > 14      6grp_total > 13      4grp_total > 54      6grp_cgc > 41      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 95
1 2 3 4 range
A 59.1
(2.3)
P 		58.1
(2)
A 		62.3
(3)
A 		35.9
(-3)
A 		26
B 101.7
(3)
P 		63.4
(0.6)
A 		51.2
(-0.2)
A 		7
(-3)
A 		95
C 94.2
(3)
P 		72.8
(1)
A 		30.6
(-3)
P 		52.5
(-0.9)
A 		64
avr 85
(3) 		64.8
(0.7) 		48
(-1.2) 		31.8
(-3) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 147
1 2 3 4 5 6 range
A 153.4
(3)
P 		53.5
(-1.1)
A 		89.3
(0.4)
P 		32.8
(-1.9)
A 		80.5
(0)
P 		6.2
(-3)
A 		147
B 145.5
(3)
P 		134.3
(2.2)
P 		121.3
(1.2)
P 		116.6
(0.8)
P 		79.3
(-1.9)
P 		65.1
(-3)
P 		80
C 34.9
(-2.7)
A 		136.2
(3)
P 		30.1
(-3)
A 		115.3
(1.8)
P 		37.7
(-2.6)
A 		47.5
(-2)
A 		106
avr 111.3
(3) 		108
(2.7) 		80.2
(0.4) 		88.2
(1.1) 		65.8
(-0.8) 		39.6
(-3) 		72
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 21.5
(3)
P 		12.6
(-0.3)
A 		16.3
(1.1)
A 		5.3
(-3)
A 		16
B 32.4
(3)
P 		15.6
(-0.3)
A 		11.9
(-1)
A 		1.5
(-3)
A 		31
C 31.2
(3)
P 		20.9
(0.2)
A 		10.7
(-2.5)
P 		8.9
(-3)
A 		22
avr 28.4
(3) 		16.4
(-0.1) 		13
(-1) 		5.2
(-3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 28.3
(3)
P 		19
(0.9)
A 		19.4
(1)
P 		8.3
(-1.5)
A 		24.3
(2.1)
P 		1.5
(-3)
A 		27
B 50.6
(3)
P 		44.7
(1.7)
P 		29.5
(-1.8)
P 		30.9
(-1.5)
P 		32.2
(-1.2)
P 		24.3
(-3)
P 		26
C 12.9
(-2.1)
A 		41.6
(3)
P 		7.7
(-3)
A 		23.2
(-0.3)
P 		12.2
(-2.2)
A 		15.9
(-1.5)
A 		34
avr 30.6
(1.7) 		35.1
(3) 		18.9
(-1.6) 		20.8
(-1) 		22.9
(-0.5) 		13.9
(-3) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187953_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.28160
Sequence Type Exemplar sequence
Representative Public ID M60.2 NCBI
Target Description M60.2_rc /REP_DB=WormBase Gene ID /WP=CE04775 /TR=Q9GYM9 /GB=AAF99996.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /REVCOMP
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Consensus/Exemplar Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade C annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076991
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076991 NCBI Caenorhabditis elegans potassium channel, KvQLT family member (kqt-2) (kqt-2) mRNA, complete cds. None
M60.5 ENSEMBL cdna:known chromosome:CEL140:X:8243073:8246307:-1 gene:M60.5 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_076990 11/11 Negative Strand Matching Probes A 
SNAP00000012320 11/11 Negative Strand Matching Probes None
GENEFINDER00000012327 11/11 Negative Strand Matching Probes None
M60.2.2 11/11 Negative Strand Matching Probes A 
M60.2.1 11/11 Negative Strand Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8242259-8243136(-) 100.0 100.0
Public Domain and Genome References
Gene Title channel protein
Gene Symbol kqt-2
Ensembl M60.5
Entrez Gene 181078 Entrez gene
SwissProt Q9GYM8 EMBL-EBI
WormBase CE30291 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6811 ion transport inferred from electronic annotation QuickGO AmiGO
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
8076 voltage-gated potassium channel complex inferred from electronic annotation QuickGO AmiGO
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5216 ion channel activity inferred from electronic annotation QuickGO AmiGO
5249 voltage-gated potassium channel activity inferred from electronic annotation QuickGO AmiGO
5249 voltage-gated potassium channel activity inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF99993 Potassium channel, kvqlt family protein 2 [Caenorhabditis elegans] ref|NP_509392.2| potassium channel, KvQLT family member (kqt-2) [Caenorhabditis elegans] 0.0
blast T28852 probable potassium channel - Caenorhabditis elegans 0.0
blast CAE74864 Hypothetical protein CBG22719 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005821 EMBL-EBI Ion transport protein 2.6E-11
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509392.2 5 88-110,125-147,160-182,227-249,299-321
M60.5 5 88-110,125-147,160-182,227-249,299-321
Sequence
Target Sequence 
>C. ELEGANS:187953_AT
gaattttccggcattctcttgaatgtagaatccaacccagttagcaaaacggttcacgac
agtaccagtggcttctccgacaaagacagacttgaatccgcagcttccaagagcttggtt
tctagcatacggaaggaaccataagttatagagttgttgacggaaatcaattggatcgct
agtagatactccttgagcttgaaggaacgtccatgcttgtttgacagcatccgaggcaat
gaagttgtcaagaaaatctttgactgcgctagcgtagtcggcggaagtcggcactgcggt
atcactgtcgtagttgaagaatggcatcaaattggcaattgtgatgtacgcgttgcttcc
caaaacagatttgtctacagttgtgaaaagggggtctttggcgttatcgtggtctgggct
cttctttgatgccatgttctggtagttcaaagtaaccaatgagtccgttcgaacatcaga
agccgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATTTTCCGGCATTCTCTTGAATG 305 325 109 Antisense
TCACGACAGTACCAGTGGCTTCTCC 658 625 162 Antisense
AAGACAGACTTGAATCCGCAGCTTC 437 153 191 Antisense
GGAACGTCCATGCTTGTTTGACAGC 550 529 312 Antisense
GAAAATCTTTGACTGCGCTAGCGTA 532 351 360 Antisense
GCTAGCGTAGTCGGCGGAAGTCGGC 75 301 376 Antisense
CACTGCGGTATCACTGTCGTAGTTG 394 197 400 Antisense
AATTGTGATGTACGCGTTGCTTCCC 541 179 445 Antisense
TCTTTGGCGTTATCGTGGTCTGGGC 204 615 503 Antisense
TCTGGGCTCTTCTTTGATGCCATGT 57 609 521 Antisense
GTCCGTTCGAACATCAGAAGCCGCA 441 475 571 Antisense
Sequence Source Affymetrix Proprietary Database