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[187951_at] seven trans-membrane receptor

Description C30F12.6 /REP_DB=WormBase Gene ID /WP=CE16886 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=seven trans-membrane receptor
Cluster 6grp_highest_10_10 > 65      6grp_high1_10_10 > 56      4grp_highest_8_8 > 14      4grp_high_8_8 > 14      6grp_highest_nonorm > 72      6grp_total > 56      4grp_total > 09      6grp_high1_sub > Cu      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 550
1 2 3 4 range
A 288
(-3)
P 		420.1
(-1)
P 		327.1
(-2.4)
P 		692.9
(3)
P 		405
B 324.3
(-3)
P 		345.4
(-2.7)
P 		362.1
(-2.5)
P 		810.9
(3)
P 		487
C 300.6
(-3)
P 		352.1
(-2.4)
P 		444
(-1.4)
P 		837.8
(3)
P 		537
avr 304.3
(-3) 		372.5
(-2.1) 		377.7
(-2.1) 		780.5
(3) 		476

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 273
1 2 3 4 5 6 range
A 423.2
(3)
P 		309.2
(-3)
P 		384.1
(0.9)
P 		357.2
(-0.5)
P 		321.2
(-2.4)
P 		376.8
(0.6)
P 		114
B 447.8
(0.4)
P 		464.3
(1.3)
P 		497.1
(3)
P 		383.7
(-3)
P 		438.3
(-0.1)
P 		487.7
(2.5)
P 		113
C 359.5
(-1.7)
P 		489.9
(1.1)
P 		318.3
(-2.6)
P 		424.5
(-0.3)
P 		301.6
(-3)
P 		574.2
(3)
P 		273
avr 410.2
(-0.3) 		421.1
(0.2) 		399.8
(-0.8) 		388.5
(-1.3) 		353.7
(-3) 		479.6
(3) 		126
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 104.9
(3)
P 		91.2
(-1.2)
P 		85.5
(-3)
P 		101.4
(1.9)
P 		19
B 103.4
(-1.7)
P 		85.3
(-2.9)
P 		83.9
(-3)
P 		172.8
(3)
P 		89
C 99.5
(-3)
P 		101.2
(-2.8)
P 		154.7
(3)
P 		141.3
(1.5)
P 		55
avr 102.6
(-1.7) 		92.6
(-3) 		108
(-1) 		138.5
(3) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 114
1 2 3 4 5 6 range
A 78
(-3)
P 		110
(3)
P 		83.7
(-1.9)
P 		90.6
(-0.6)
P 		96.9
(0.5)
P 		91.7
(-0.4)
P 		32
B 155.8
(1)
P 		154.5
(1)
P 		120.7
(-1.6)
P 		101.6
(-3)
P 		178.1
(2.7)
P 		181.9
(3)
P 		80
C 133.2
(-0.2)
P 		149.5
(0.7)
P 		81
(-3)
P 		85.4
(-2.8)
P 		97.6
(-2.1)
P 		192.2
(3)
P 		111
avr 122.3
(-0.2) 		138
(1.3) 		95.1
(-2.8) 		92.5
(-3) 		124.2
(0) 		155.3
(3) 		63
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187951_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1270
Sequence Type Exemplar sequence
Representative Public ID C30F12.6 NCBI
Target Description C30F12.6 /REP_DB=WormBase Gene ID /WP=CE16886 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=seven trans-membrane receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059589(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059589 NCBI Caenorhabditis elegans C30F12.6 (C30F12.6) mRNA, complete cds. 11/11 None
SNAP00000017408 ENSEMBL cdna:SNAP chromosome:CEL140:I:6997230:6999973:1 11/11 None
GENEFINDER00000017415 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6997230:6999973:1 11/11 None
C30F12.6 ENSEMBL cdna:known chromosome:CEL140:I:6997199:7000245:1 gene:C30F12.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6997160-6999904(+) 100.0 100.0
Public Domain and Genome References
Gene Title seven trans-membrane receptor
Ensembl C30F12.6
Entrez Gene 183056 Entrez gene
SwissProt Q95YD7 EMBL-EBI
WormBase CE16886 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.2003.1.S1_AT similar to thyrotropin-releasing hormone receptor cfa
CHICKEN:GGA.457.1.S1_AT thyrotropin-releasing hormone receptor gga
HG-U133_PLUS_2:211438_AT thyrotropin-releasing hormone receptor hs
HG-U133A:211438_AT thyrotropin-releasing hormone receptor hs
HG-FOCUS:211438_AT thyrotropin-releasing hormone receptor hs
HG-U133A_2:211438_AT thyrotropin-releasing hormone receptor hs
HG-U95AV2:750_AT thyrotropin-releasing hormone receptor hs
HC-G110:750_AT thyrotropin-releasing hormone receptor hs
HUGENEFL:D85376_AT thyrotropin-releasing hormone receptor hs
U133_X3P:G577631_3P_AT thyrotropin-releasing hormone receptor hs
MOUSE430A_2:1449571_AT thyrotropin releasing hormone receptor mm
MOE430A:1449571_AT thyrotropin releasing hormone receptor mm
MOUSE430_2:1449571_AT thyrotropin releasing hormone receptor mm
MU11KSUBA:M94384_S_AT thyrotropin releasing hormone receptor mm
MG-U74AV2:97735_AT thyrotropin releasing hormone receptor mm
MOE430A:1449572_AT thyrotropin releasing hormone receptor mm
MOUSE430A_2:1449572_AT thyrotropin releasing hormone receptor mm
MOUSE430_2:1449572_AT thyrotropin releasing hormone receptor mm
RAE230A:1370736_S_AT thyrotropin releasing hormone receptor rn
RAT230_2:1370736_S_AT thyrotropin releasing hormone receptor rn
RG-U34A:S69160_S_AT thyrotropin releasing hormone receptor rn
RG-U34A:D17469_AT thyrotropin releasing hormone receptor rn
RG-U34A:S69161_S_AT thyrotropin releasing hormone receptor rn
RAE230A:1369751_AT thyrotropin releasing hormone receptor rn
RAT230_2:1369751_AT thyrotropin releasing hormone receptor rn
RAE230A:1387682_AT thyrotropin releasing hormone receptor rn
RAT230_2:1387682_AT thyrotropin releasing hormone receptor rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
1584 rhodopsin-like receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK85452 Hypothetical protein C30F12.6 [Caenorhabditis elegans] ref|NP_491990.1| C30F12.6 [Caenorhabditis elegans] 0.0
blast CAE60346 Hypothetical protein CBG03939 [Caenorhabditis briggsae] 0.0
blast CAD12658 thyrotropin-releasing hormone receptor 1 [Xenopus laevis] 4.0E-59
Protein Domains
Database ID Description E-Value
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 2.8E-69
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 3.3E-69
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491990.1 7 36-58,71-93,108-130,151-173,203-225,279-298,318-340
C30F12.6 7 36-58,71-93,108-130,151-173,203-225,279-298,318-340
SNAP00000017408 7 36-58,71-93,108-130,151-173,203-225,282-301,321-343
GENEFINDER00000017415 7 36-58,71-93,108-130,151-173,203-225,279-298,318-340
Sequence
Target Sequence 
>C. ELEGANS:187951_AT
gatagttttccttcttgatttcgtcctgtggtacgtgcttccaattatgtgcgacatcat
tatctacgcaaaaatcggtatcactttgagtcaatgtggagataagattaagaaatcggt
gaagccgaaaataccaaatgaagtgatcgtcgaaaaatcaaagacatcgtcgacttcaat
gggacattattccggaagagattctcatatttcggggaaacggaactcggcaaaaggaag
aaatcaggttgtaaaaatgcttgcaatcgttgtggccgtcttcgcagtgtgttggcttcc
ttatcgtggaatggttgtctataattcatttgtatcggatccaaagtattcatggagtcc
tgattggtatatcaatttgtcaaagacacttgtattcatcaactgtgctattaatccaat
actatacaacttgatgtctgctagattccgtgctgcattcagatcgttgttatcgaaaag
aaagaattccggatttaaatcaacagctttgaaccaacgtcaccgattaaacacaatgga
tataatgtcatcagctgaaccaaaatctcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATAGTTTTCCTTCTTGATTTCGTC 517 425 600 Antisense
TGATTTCGTCCTGTGGTACGTGCTT 516 563 615 Antisense
ACGTGCTTCCAATTATGTGCGACAT 562 95 632 Antisense
GACATCGTCGACTTCAATGGGACAT 326 369 762 Antisense
AAATGCTTGCAATCGTTGTGGCCGT 618 119 854 Antisense
TTGGCTTCCTTATCGTGGAATGGTT 270 709 891 Antisense
AGACACTTGTATTCATCAACTGTGC 582 71 983 Antisense
ACTTGATGTCTGCTAGATTCCGTGC 335 103 1028 Antisense
GATTCCGTGCTGCATTCAGATCGTT 370 433 1043 Antisense
CAACAGCTTTGAACCAACGTCACCG 209 159 1100 Antisense
GTCATCAGCTGAACCAAAATCTCCA 659 467 1146 Antisense
Sequence Source Affymetrix Proprietary Database