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[187946_at]

Description M110.2 /REP_DB=WormBase Gene ID /WP=CE02275 /GEN=twk-14 /TR=Q21529 /GB=CAA90259.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 02      4grp_highest_8_8 > 63      4grp_high_8_8 > 27      6grp_total > 24      4grp_total > 37      6grp_cgc > 20      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 78.4
(2.4)
P
57.1
(-3)
P
69.5
(0.2)
P
80.6
(3)
P
24
B 61
(-0.7)
P
76.5
(3)
P
55.7
(-2)
P
51.3
(-3)
P
25
C 70.6
(2.7)
P
71.1
(3)
P
62.2
(-3)
P
67.9
(0.8)
P
9
avr 70
(3)
68.2
(1.6)
62.5
(-3)
66.6
(0.3)
8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 99.6
(3)
P
65.8
(0.6)
P
81.4
(1.7)
P
54.3
(-0.2)
P
34.4
(-1.6)
P
13.8
(-3)
A
86
B 88.5
(3)
P
66.8
(1)
P
53.2
(-0.3)
P
30.3
(-2.4)
A
24.8
(-2.9)
A
24.1
(-3)
P
64
C 81.3
(3)
P
81.1
(3)
P
73.5
(2.1)
P
70.2
(1.8)
P
27.9
(-3)
A
44.2
(-1.2)
P
53
avr 89.8
(3)
71.2
(1.2)
69.4
(1)
51.6
(-0.7)
29
(-2.8)
27.4
(-3)
62
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 28.5
(3)
P
12.4
(-2.8)
P
18.2
(-0.7)
P
11.8
(-3)
P
17
B 19.5
(3)
P
18.9
(2.6)
P
12.9
(-1.6)
P
10.9
(-3)
P
9
C 23.4
(3)
P
20.4
(1.5)
P
21.7
(2.1)
P
11.5
(-3)
P
12
avr 23.8
(3)
17.2
(-0.2)
17.6
(0)
11.4
(-3)
12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 18.4
(1.5)
P
23.4
(3)
P
17.7
(1.3)
P
13.8
(0.1)
P
10.4
(-0.9)
P
3.4
(-3)
A
20
B 30.8
(3)
P
22.2
(0.7)
P
12.9
(-1.7)
P
8
(-3)
A
10.1
(-2.4)
A
9
(-2.7)
P
23
C 30.2
(3)
P
24.7
(1.4)
P
18.7
(-0.3)
P
14.1
(-1.6)
P
9
(-3)
A
14.8
(-1.4)
P
21
avr 26.5
(3)
23.4
(1.9)
16.4
(-0.5)
12
(-2)
9.8
(-2.8)
9.1
(-3)
17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187946_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3195
Sequence Type Exemplar sequence
Representative Public ID M110.2 NCBI
Target Description M110.2 /REP_DB=WormBase Gene ID /WP=CE02275 /GEN=twk-14 /TR=Q21529 /GB=CAA90259.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063326(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_063326 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-3) (twk-3) mRNA, complete cds. 11/11 None
SNAP00000006398 ENSEMBL cdna:SNAP chromosome:CEL140:II:8210585:8212674:-1 10/11 None
GENEFINDER00000006413 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8210585:8212674:-1 11/11 None
M110.2 ENSEMBL cdna:known chromosome:CEL140:II:8210585:8212674:-1 gene:M110.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8210581-8212671(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol twk-3
Ensembl M110.2
Entrez Gene 174321 Entrez gene
SwissProt Q21529 EMBL-EBI
WormBase CE02275 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5267 potassium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA90259 Hypothetical protein M110.2 [Caenorhabditis elegans] ref|NP_495727.1| TWiK family of potassium channels family member (twk-3) [Caenorhabditis elegans] 0.0
blast CAE57706 Hypothetical protein CBG00713 [Caenorhabditis briggsae] 1.0E-176
blast CAE57706 Hypothetical protein CBG00713 [Caenorhabditis briggsae] 1.0E-163
blast CAE71906 Hypothetical protein CBG18967 [Caenorhabditis briggsae] 4.0E-52
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495727.1 5 38-60,134-156,160-182,202-224,256-278
M110.2 5 38-60,134-156,160-182,202-224,256-278
SNAP00000006398 5 38-60,134-156,160-182,202-224,239-261
GENEFINDER00000006413 5 38-60,134-156,160-182,202-224,256-278
Sequence
Target Sequence
>C. ELEGANS:187946_AT
cactgactcttgttactatggctgatactggcaagtttgctgcccaattggtaacacgtt
ggtttggagataataatatggcaattcctgcagctatattcgtttgtcttctctttgctt
atccacttgttgtcggatttattttatgttcaacttctaatatcacatacttggattctg
tttatttttcattgacgtcaatttttacaatagggtttggagatttgacacctgacatga
acgtaatccatatggttttatttctggctgttggcgtaattcttgtaacaattactctcg
acattgtagctgctgaaatgattgatagagtacattacatgggtcggcatgttggaaaag
ctaaagaattagctggaaaaatgttccaattggctcaatcattaaatatgaagcagggat
tggtgtcaggagtcggacaattgcatgcattggcaagatttggaatgttggttggcagag
aggaagtcgacaaaactcaagaagacgggattattgcattttctcccgatgttatggatg
gactagaattcatggatactctatccatttatagccggaggagtcgacgatcagc


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
CACTGACTCTTGTTACTATGGCTGA 149 199 536 Antisense
GCAAGTTTGCTGCCCAATTGGTAAC 615 319 566 Antisense
TATGGCAATTCCTGCAGCTATATTC 432 655 612 Antisense
AGCTATATTCGTTTGTCTTCTCTTT 176 79 627 Antisense
TCTTTGCTTATCCACTTGTTGTCGG 484 615 647 Antisense
GAGATTTGACACCTGACATGAACGT 7 397 755 Antisense
TATGGTTTTATTTCTGGCTGTTGGC 449 655 786 Antisense
CTGGCTGTTGGCGTAATTCTTGTAA 648 241 799 Antisense
TTACTCTCGACATTGTAGCTGCTGA 205 681 827 Antisense
GGATTATTGCATTTTCTCCCGATGT 336 509 1043 Antisense
ATAGCCGGAGGAGTCGACGATCAGC 623 21 1106 Antisense
Sequence Source Affymetrix Proprietary Database