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[187945_at]

Description K08E7.2 /REP_DB=WormBase Gene ID /WP=CE23864 /GEN=hsb-1 /TR=Q9U3B7 /GB=CAB01233.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 39      6grp_high1_10_10 > 82      4grp_highest_8_8 > 09      4grp_high_8_8 > 53      6grp_highest_nonorm > 55      6grp_total > 73      4grp_total > 59      6grp_highest_sub > 04      6grp_cgc > 76      6grp_high1_sub > 04      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S5      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1007
1 2 3 4 range
A 794
(0.4)
P 		982.6
(2.2)
P 		1061.2
(3)
P 		442.4
(-3)
P 		619
B 786.1
(-1.2)
P 		1403
(3)
P 		1246.8
(1.9)
P 		511.6
(-3)
P 		891
C 897.1
(1.1)
P 		1039.5
(2.3)
P 		1125
(3)
P 		396
(-3)
P 		729
avr 825.7
(0.2) 		1141.7
(3) 		1144.3
(3) 		450
(-3) 		694

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1061
1 2 3 4 5 6 range
A 1173
(-2.8)
P 		1145.8
(-3)
P 		1766.6
(2.6)
P 		1477.5
(-0)
P 		1810.1
(3)
P 		1170.4
(-2.8)
P 		664
B 1303.1
(-2.6)
P 		1247.5
(-3)
P 		1998
(2.5)
P 		1860.3
(1.5)
P 		2065.7
(3)
P 		1642.9
(-0.1)
P 		818
C 1004.8
(-3)
P 		1454
(0.3)
P 		1258.2
(-1.1)
P 		1820.6
(3)
P 		1564.3
(1.1)
P 		1711.7
(2.2)
P 		816
avr 1160.3
(-3) 		1282.4
(-1.9) 		1674.3
(1.7) 		1719.5
(2.1) 		1813.4
(3) 		1508.3
(0.2) 		653
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 327
1 2 3 4 range
A 289.1
(3)
P 		213.3
(1)
P 		277.4
(2.7)
P 		64.8
(-3)
P 		224
B 250.7
(0.6)
P 		346.5
(3)
P 		288.9
(1.5)
P 		109
(-3)
P 		238
C 297
(1.2)
P 		298.9
(1.3)
P 		392.1
(3)
P 		66.8
(-3)
P 		325
avr 278.9
(2) 		286.2
(2.2) 		319.5
(3) 		80.2
(-3) 		239

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 623
1 2 3 4 5 6 range
A 216.2
(-3)
P 		407.5
(0.5)
P 		384.7
(0.1)
P 		374.7
(-0.1)
P 		546.2
(3)
P 		285
(-1.7)
P 		330
B 453.4
(-2.5)
P 		415.3
(-3)
P 		485.2
(-2)
P 		492.7
(-1.9)
P 		839.3
(3)
P 		612.6
(-0.2)
P 		424
C 372.4
(-1.8)
P 		443.7
(-0.1)
P 		320.2
(-3)
P 		366.1
(-1.9)
P 		506.2
(1.4)
P 		573
(3)
P 		253
avr 347.3
(-3) 		422.2
(-1.4) 		396.7
(-2) 		411.2
(-1.6) 		630.6
(3) 		490.2
(0) 		283
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187945_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10119
Sequence Type Exemplar sequence
Representative Public ID K08E7.2 NCBI
Target Description K08E7.2 /REP_DB=WormBase Gene ID /WP=CE23864 /GEN=hsb-1 /TR=Q9U3B7 /GB=CAB01233.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070005(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070005 NCBI Caenorhabditis elegans Heat Shock factor Binding protein family member (hsb-1) (hsb-1) mRNA, complete cds. 11/11 None
SNAP00000042643 ENSEMBL cdna:SNAP chromosome:CEL140:IV:12566887:12567183:-1 11/11 None
GENEFINDER00000042658 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:12566887:12567183:-1 11/11 None
K08E7.2 ENSEMBL cdna:known chromosome:CEL140:IV:12565207:12567183:-1 gene:K08E7.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12566892-12567189(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol hsb-1
Ensembl K08E7.2
Entrez Gene 178208 Entrez gene
SwissProt Q9U3B7 EMBL-EBI
WormBase CE23864 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01233 Hypothetical protein K08E7.2 [Caenorhabditis elegans] ref|NP_502406.1| Heat Shock factor Binding protein family member (hsb-1) [Caenorhabditis elegans] 1.0E-29
blast CAE67912 Hypothetical protein CBG13509 [Caenorhabditis briggsae] 1.0E-17
Protein Domains
Database ID Description E-Value
Pfam IPR009643 EMBL-EBI Heat shock factor binding 1 3.8E-30
Sequence
Target Sequence 
>C. ELEGANS:187945_AT
atgtccgatgagaagtctaccacaccaaccgctcaactcgacgctccagccgatggaaat
atgaatgatttgacgtcgttgatacaaggagttctgcaacagacacaggatcgattccag
cacatgtcggatcagattattcggcgaatcgatgatatgacaacgagaatcgatgatttg
gagaagaatatcaacgatttgctgcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTCCGATGAGAAGTCTACCACAC 154 49 13 Antisense
CGATGAGAAGTCTACCACACCAACC 483 249 18 Antisense
TCGACGCTCCAGCCGATGGAAATAT 14 601 50 Antisense
ATATGAATGATTTGACGTCGTTGAT 616 19 71 Antisense
GACGTCGTTGATACAAGGAGTTCTG 618 377 84 Antisense
GGAGTTCTGCAACAGACACAGGATC 457 517 100 Antisense
GACACAGGATCGATTCCAGCACATG 287 367 114 Antisense
GATTCCAGCACATGTCGGATCAGAT 641 433 125 Antisense
GTCGGATCAGATTATTCGGCGAATC 173 473 138 Antisense
TCGGCGAATCGATGATATGACAACG 187 607 153 Antisense
GAAGAATATCAACGATTTGCTGCAA 153 341 195 Antisense
Sequence Source Affymetrix Proprietary Database