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[187944_at] FMRFamide-like peptide

Description C18D1.3 /REP_DB=WormBase Gene ID /WP=CE01503 /GEN=flp-4 /TR=Q09480 /GB=CAA88434.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 26      4grp_highest_8_8 > 39      4grp_high_8_8 > 12      6grp_highest_nonorm > 51      6grp_total > 25      4grp_total > 12      6grp_highest_sub > 01_02      6grp_cgc > 24      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1998
1 2 3 4 range
A 881.3
(-1.5)
P 		519.4
(-2.6)
P 		398.8
(-3)
P 		2365.5
(3)
P 		1967
B 1093.5
(-0.5)
P 		524
(-2.5)
P 		367.3
(-3)
P 		2130.1
(3)
P 		1763
C 1047
(-0.6)
P 		548
(-2.5)
P 		400.9
(-3)
P 		2024.6
(3)
P 		1624
avr 1007.3
(-0.9) 		530.5
(-2.5) 		389
(-3) 		2173.4
(3) 		1784

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 412
1 2 3 4 5 6 range
A 667.5
(2.8)
P 		680.7
(3)
P 		632
(2.2)
P 		517.7
(0.2)
P 		336.9
(-3)
P 		394.5
(-2)
P 		344
B 748.5
(3)
P 		688.5
(1.8)
P 		444.7
(-3)
P 		470.9
(-2.5)
P 		489
(-2.1)
P 		585.3
(-0.2)
P 		304
C 658.2
(2.2)
P 		702.8
(3)
P 		540.8
(0.1)
P 		369.9
(-3)
P 		489
(-0.9)
P 		556.5
(0.4)
P 		333
avr 691.4
(3) 		690.7
(3) 		539.2
(-0.6) 		452.8
(-2.7) 		438.3
(-3) 		512.1
(-1.3) 		253
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 369
1 2 3 4 range
A 320.9
(2.4)
P 		112.7
(-2.8)
P 		104.2
(-3)
P 		346.3
(3)
P 		242
B 348.7
(1.3)
P 		129.4
(-2.3)
P 		85.1
(-3)
P 		453.9
(3)
P 		369
C 346.6
(3)
P 		157.6
(-2.5)
P 		139.7
(-3)
P 		341.4
(2.8)
P 		207
avr 338.7
(2.1) 		133.2
(-2.5) 		109.7
(-3) 		380.5
(3) 		271

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 186
1 2 3 4 5 6 range
A 123.1
(-1.9)
P 		242.1
(3)
P 		137.6
(-1.3)
P 		131.3
(-1.6)
P 		101.7
(-2.8)
P 		96
(-3)
P 		146
B 260.4
(3)
P 		229.2
(1.8)
P 		108
(-3)
P 		124.7
(-2.3)
P 		198.7
(0.6)
P 		218.3
(1.3)
P 		152
C 244
(3)
P 		214.5
(2)
P 		137.6
(-0.8)
P 		74.4
(-3)
P 		158.2
(-0)
P 		186.3
(1)
P 		170
avr 209.2
(2) 		228.6
(3) 		127.7
(-2.1) 		110.1
(-3) 		152.9
(-0.8) 		166.9
(-0.1) 		119
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187944_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3217
Sequence Type Exemplar sequence
Representative Public ID C18D1.3 NCBI
Target Description C18D1.3 /REP_DB=WormBase Gene ID /WP=CE01503 /GEN=flp-4 /TR=Q09480 /GB=CAA88434.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063772(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063772 NCBI Caenorhabditis elegans FMRF-Like Peptide family member (flp-4) (flp-4) mRNA, complete cds. 11/11 None
SNAP00000007489 ENSEMBL cdna:SNAP chromosome:CEL140:II:10054550:10055269:1 11/11 None
GENEFINDER00000007495 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10054550:10055269:1 11/11 None
C18D1.3 ENSEMBL cdna:known chromosome:CEL140:II:10054536:10055269:1 gene:C18D1.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10054546-10055266(+) 100.0 100.0
Public Domain and Genome References
Gene Title FMRFamide-like peptide
Gene Symbol flp-4
Ensembl C18D1.3
Entrez Gene 174568 Entrez gene
SwissProt Q09480 EMBL-EBI
WormBase CE01503 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA88434 Hypothetical protein C18D1.3 [Caenorhabditis elegans] ref|NP_496173.1| FMRF-Like Peptide family member (flp-4) [Caenorhabditis elegans] gb|AAC08941.1| FMRFamide-like peptide 4 [Caenorhabditis elegans] 6.0E-37
blast CAE59354 Hypothetical protein CBG02703 [Caenorhabditis briggsae] 9.0E-20
Sequence
Target Sequence 
>C. ELEGANS:187944_AT
tcctcttcccttaaaacattcatcttctccttgctttttgccacgttgcttgcactcaca
gcagctcatccaccgtcatctggtgaagaaattgctgagcaagaagagaagaatattgca
agtccagatgaacttattccggaaattgtcgagcagcaaaacttctggccaccagtgcat
cttcgtggattacgatcatcaaacggaaaaccaaccttcatcagattcggcaagcgggct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCTCTTCCCTTAAAACATTCATCT 389 593 25 Antisense
TTAAAACATTCATCTTCTCCTTGCT 601 683 35 Antisense
TCATCCACCGTCATCTGGTGAAGAA 143 623 90 Antisense
GCAAGTCCAGATGAACTTATTCCGG 130 321 142 Antisense
CTTATTCCGGAAATTGTCGAGCAGC 564 221 157 Antisense
GTCGAGCAGCAAAACTTCTGGCCAC 627 473 172 Antisense
AAACTTCTGGCCACCAGTGCATCTT 617 127 183 Antisense
ACCAGTGCATCTTCGTGGATTACGA 239 87 195 Antisense
GATCATCAAACGGAAAACCAACCTT 123 421 218 Antisense
ACCAACCTTCATCAGATTCGGCAAG 378 83 234 Antisense
CTTCATCAGATTCGGCAAGCGGGCT 208 219 240 Antisense
Sequence Source Affymetrix Proprietary Database