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[187941_at]

Description W01G7.1 /REP_DB=WormBase Gene ID /WP=CE18985 /GEN=daf-5 /TR=Q9XVH7 /GB=CAB03453.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 17      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 70      6grp_total > 16      4grp_total > 46      6grp_highest_sub > De      6grp_cgc > 12      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 997
1 2 3 4 range
A 1160.1
(3)
P 		465.9
(-1.2)
P 		162.7
(-3)
P 		260.1
(-2.4)
P 		997
B 1107.9
(3)
P 		370.5
(-1.8)
P 		179.3
(-3)
P 		336.3
(-2)
P 		929
C 1056.6
(3)
P 		392.9
(-1.6)
P 		191.9
(-2.9)
P 		184.4
(-3)
P 		872
avr 1108.2
(3) 		409.8
(-1.5) 		178
(-3) 		260.3
(-2.5) 		930

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 589
1 2 3 4 5 6 range
A 349.5
(3)
P 		346.4
(2.9)
P 		109.5
(-3)
P 		147.9
(-2)
P 		126
(-2.6)
P 		207.4
(-0.6)
P 		240
B 435
(3)
P 		284.9
(0.2)
P 		185.8
(-1.6)
P 		166.3
(-1.9)
P 		120.3
(-2.8)
P 		107.7
(-3)
P 		327
C 686.6
(3)
P 		232.6
(-1.6)
P 		200.7
(-1.9)
P 		121.7
(-2.8)
P 		159.4
(-2.4)
P 		97.3
(-3)
P 		589
avr 490.4
(3) 		288
(-0.4) 		165.3
(-2.5) 		145.3
(-2.8) 		135.2
(-3) 		137.5
(-3) 		355
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 391
1 2 3 4 range
A 422.4
(3)
P 		101.1
(-2)
P 		42.5
(-2.9)
P 		38.1
(-3)
P 		384
B 353.3
(3)
P 		91.5
(-2)
P 		41.6
(-3)
P 		71.7
(-2.4)
P 		312
C 349.8
(3)
P 		113
(-1.5)
P 		66.9
(-2.3)
P 		31.1
(-3)
P 		319
avr 375.2
(3) 		101.9
(-2) 		50.3
(-2.9) 		47
(-3) 		328

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 231
1 2 3 4 5 6 range
A 64.4
(-0.5)
P 		123.2
(3)
P 		23.8
(-3)
P 		37.5
(-2.2)
P 		38
(-2.1)
P 		50.5
(-1.4)
P 		99
B 151.4
(3)
P 		94.8
(-0.1)
P 		45.1
(-2.7)
P 		44
(-2.8)
P 		48.9
(-2.5)
P 		40.2
(-3)
P 		111
C 254.5
(3)
P 		71
(-1.8)
P 		51.1
(-2.3)
P 		24.5
(-3)
P 		51.6
(-2.3)
P 		32.6
(-2.8)
P 		230
avr 156.8
(3) 		96.3
(0) 		40
(-2.8) 		35.3
(-3) 		46.2
(-2.5) 		41.1
(-2.7) 		122
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187941_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2918
Sequence Type Exemplar sequence
Representative Public ID W01G7.1 NCBI
Target Description W01G7.1 /REP_DB=WormBase Gene ID /WP=CE18985 /GEN=daf-5 /TR=Q9XVH7 /GB=CAB03453.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064540(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064540 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-5) (daf-5) mRNA, complete cds. 11/11 None
SNAP00000032215 ENSEMBL cdna:SNAP chromosome:CEL140:II:14033729:14037606:-1 11/11 None
GENEFINDER00000032220 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14033729:14037606:-1 11/11 None
W01G7.1 ENSEMBL cdna:known chromosome:CEL140:II:14033108:14037613:-1 gene:W01G7.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14033725-14037603(-) 97.98 100.0
Public Domain and Genome References
Gene Title
Gene Symbol daf-5
Ensembl W01G7.1
Entrez Gene 175054 Entrez gene
SwissProt Q9XVH7 EMBL-EBI
WormBase CE18985 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03453 Hypothetical protein W01G7.1 [Caenorhabditis elegans] ref|NP_496941.1| abnormal DAuer Formation family member (daf-5) [Caenorhabditis elegans] gb|AAR00670.1| abnormal DAuer Formation DAF-5, a Ski oncogene homolog involved in a neuronal TGF beta pathway (71.0 kD) (daf-5) [Caenorhabditis elegans] 0.0
blast CAE73392 Hypothetical protein CBG20832 [Caenorhabditis briggsae] 9.0E-87
Sequence
Target Sequence 
>C. ELEGANS:187941_AT
gcgtcagcgaacttcgacgtttccaaaggagagttcgacaacatgcgccacttcgacaat
gcctccaaagccacgcgacaagaaatccagcagctcgccgagcaattcgccaatctcgat
cgcgacgccaaacttttgactccaatggagttcgtgttaagagagcacgcgttgctcaag
aatgtctcggcagacgcgatacgtgtactgtgtaaccgtccgccactgccgccactgccg
ccaccgccaccgccaccaaaaccaaagccggctccggtacagccgatctcgttgggaaat
atcaactttgtggcgttagcccagcagctgatcgcgtctgggatcaagcttcctctgccg
attgtgacaccgccagtggtttctacgccggcgccagtgattacgccgattcctgcagcg
ttgccgatctcgccgaattctgattttttgaaacaacaactctccacggccatgtcatct
ccagctcttctctccctctacccgaagctcaccgccggtgcctatgagcaattggccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGTCAGCGAACTTCGACGTTTCCA 596 291 1327 Antisense
AGGAGAGTTCGACAACATGCGCCAC 170 57 1353 Antisense
GCCACGCGACAAGAAATCCAGCAGC 635 279 1396 Antisense
TCGCGACGCCAAACTTTTGACTCCA 144 599 1446 Antisense
GCACGCGTTGCTCAAGAATGTCTCG 504 315 1491 Antisense
GTCTCGGCAGACGCGATACGTGTAC 633 469 1510 Antisense
TACGTGTACTGTGTAACCGTCCGCC 535 637 1526 Antisense
ACTTTGTGGCGTTAGCCCAGCAGCT 587 101 1631 Antisense
CCAGCAGCTGATCGCGTCTGGGATC 314 273 1647 Antisense
TGATTACGCCGATTCCTGCAGCGTT 530 563 1724 Antisense
GCCGGTGCCTATGAGCAATTGGCCC 15 283 1840 Antisense
Sequence Source Affymetrix Proprietary Database