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[187939_at] FRG1-like actin crosslinking protein

Description ZK1010.3 /REP_DB=WormBase Gene ID /WP=CE15499 /TR=O18282 /GB=CAB04969.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=FRG1
Cluster 6grp_highest_10_10 > 67      6grp_high1_10_10 > 86      4grp_highest_8_8 > 27      4grp_high_8_8 > 63      6grp_highest_nonorm > 94      6grp_total > 76      4grp_total > 56      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 106
1 2 3 4 range
A 424.4
(-3)
P 		425.7
(-2.9)
P 		460.6
(-0.1)
P 		499.7
(3)
P 		75
B 463.7
(3)
P 		394
(-3)
P 		438.6
(0.8)
P 		394.8
(-2.9)
P 		70
C 430.5
(-1.8)
P 		479.4
(3)
P 		474
(2.5)
P 		417.8
(-3)
P 		62
avr 439.5
(-1.4) 		433
(-3) 		457.7
(3) 		437.4
(-1.9) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 586
1 2 3 4 5 6 range
A 381.7
(-3)
P 		388.9
(-2.8)
P 		512.6
(-0)
P 		493
(-0.5)
P 		414.8
(-2.2)
P 		645.9
(3)
P 		264
B 415.8
(-2.8)
P 		402.8
(-3)
P 		505.1
(-1.5)
P 		592.7
(-0.2)
P 		603.2
(-0.1)
P 		817.1
(3)
P 		414
C 453.2
(-2.5)
P 		406.3
(-3)
P 		465.1
(-2.4)
P 		418.8
(-2.9)
P 		492.6
(-2.1)
P 		967.5
(3)
P 		561
avr 416.9
(-2.7) 		399.3
(-3) 		494.3
(-1.6) 		501.5
(-1.5) 		503.5
(-1.5) 		810.2
(3) 		411
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 95
1 2 3 4 range
A 154.5
(3)
P 		92.4
(-1.6)
P 		120.4
(0.5)
P 		73.2
(-3)
P 		81
B 147.9
(3)
P 		97.3
(-1.8)
P 		101.6
(-1.4)
P 		84.1
(-3)
P 		64
C 142.5
(1.6)
P 		137.8
(1.3)
P 		165.2
(3)
P 		70.4
(-3)
P 		95
avr 148.3
(3) 		109.2
(-0.2) 		129.1
(1.4) 		75.9
(-3) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 254
1 2 3 4 5 6 range
A 70.4
(-3)
P 		138.3
(1.7)
P 		111.6
(-0.2)
P 		125
(0.8)
P 		125.2
(0.8)
P 		157.3
(3)
P 		87
B 144.7
(-2.3)
P 		134.1
(-2.6)
P 		122.7
(-3)
P 		157
(-1.9)
P 		245.1
(1)
P 		304.7
(3)
P 		182
C 168
(-0.9)
P 		124
(-2)
P 		118.4
(-2.1)
P 		84.2
(-3)
P 		159.4
(-1.1)
P 		323.9
(3)
P 		240
avr 127.7
(-2.6) 		132.1
(-2.4) 		117.6
(-3) 		122.1
(-2.8) 		176.6
(-0.5) 		262
(3) 		144
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187939_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6527
Sequence Type Exemplar sequence
Representative Public ID ZK1010.3 NCBI
Target Description ZK1010.3 /REP_DB=WormBase Gene ID /WP=CE15499 /TR=O18282 /GB=CAB04969.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=FRG1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067295(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067295 NCBI Caenorhabditis elegans ZK1010.3 (ZK1010.3) mRNA, complete cds. 11/11 None
SNAP00000035099 ENSEMBL cdna:SNAP chromosome:CEL140:III:12978691:12979628:1 11/11 None
GENEFINDER00000035108 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:12978691:12979628:1 11/11 None
ZK1010.3.1 ENSEMBL cdna:known chromosome:CEL140:III:12978677:12979658:1 gene:ZK1010.3 11/11 None
ZK1010.3.2 ENSEMBL cdna:known chromosome:CEL140:III:12978678:12979639:1 gene:ZK1010.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:12978463-12979624(+) 100.0 100.0
Public Domain and Genome References
Gene Title FRG1-like actin crosslinking protein
Ensembl ZK1010.3
Entrez Gene 176719 Entrez gene
SwissProt O18282 EMBL-EBI
WormBase CE37862 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.11576.1.S1_AT similar to FRG1 protein (FSHD region gene 1 protein) cfa
DROSOPHILA_2:1631101_AT dm
DROSGENOME1:142613_AT dm
CHICKEN:GGAAFFX.8661.1.S1_AT similar to FSHD region gene 1 gga
HG-U95E:72108_AT FSHD region gene 1 hs
HG-U133_PLUS_2:204145_AT FSHD region gene 1 hs
HG-U133A:204145_AT FSHD region gene 1 hs
HG-FOCUS:204145_AT FSHD region gene 1 hs
HG-U133A_2:204145_AT FSHD region gene 1 hs
HU35KSUBC:RC_AA058589_F_AT FSHD region gene 1 hs
HUGENEFL:L76159_AT FSHD region gene 1 hs
U133_X3P:G4758403_3P_X_AT FSHD region gene 1 hs
U133_X3P:HS.203772.1.S1_3P_A_AT FSHD region gene 1 hs
HG-U133_PLUS_2:235535_X_AT FSHD region gene 1 hs
HG-U133B:235535_X_AT FSHD region gene 1 hs
HG-U95AV2:38923_AT FSHD region gene 1 hs
HG-U95C:63686_AT FSHD region gene 1 hs
HG-U133_PLUS_2:237589_AT FSHD region gene 1 hs
HG-U133B:237589_AT FSHD region gene 1 hs
HG-U95B:47308_AT FSHD region gene 1 hs
U133_X3P:HS.14295.0.A1_3P_AT FSHD region gene 1 hs
MU11KSUBA:U62105_S_AT FSHD region gene 1 mm
MG-U74AV2:100978_AT FSHD region gene 1 mm
MG-U74CV2:166522_R_AT FSHD region gene 1 mm
MOE430A:1417253_AT FSHD region gene 1 mm
MOUSE430A_2:1417253_AT FSHD region gene 1 mm
MOUSE430_2:1417253_AT FSHD region gene 1 mm
MU19KSUBA:TC19436_AT FSHD region gene 1, mRNA (cDNA clone MGC:5909 IMAGE:3487998) mm
MU19KSUBA:TC19436_G_AT FSHD region gene 1, mRNA (cDNA clone MGC:5909 IMAGE:3487998) mm
RAE230B:1385601_AT FSHD region gene 1 (predicted) rn
RAT230_2:1385601_AT FSHD region gene 1 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAB04969 Hypothetical protein ZK1010.3 [Caenorhabditis elegans] ref|NP_499696.2| ZK1010.3 [Caenorhabditis elegans] sp|O18282|FRG1_CAEEL FRG1 protein homolog 1.0E-122
blast CAE69562 Hypothetical protein CBG15774 [Caenorhabditis briggsae] 1.0E-106
Protein Domains
Database ID Description E-Value
Pfam IPR010414 EMBL-EBI FRG1-like 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:187939_AT
gtcgccatcgaagttgccagtggagccggctcaacgcgaacttatgtcgctgcaatggac
aacggaaaattcacaattggatttccacatccggagggagaaggcccgaatcctgaggaa
attttcgcgctcgtcaaaactccagacgatagtaaaatcagtttgaagacaggattcgga
agatatgttggagtggacagcgaatatcaattggttgccatggctgaagctattggatcc
cgcgagcaattcgtgttggttttccaggagggaaaaaccgcgttccaagccgtctcctca
ccacttttcctctctacagttccaaataaagaggggcatatttatgtggctagtagaacc
gctacggagaatgaaatggttaatatccgtacagacgctatacaagaaggcccagtcgat
tggagatctgtggaagaccgcaaaaacgctcgggagtgtgagacggcctacgtgaagatg
tatcagcactcgaaggttgatctcaagaaccgacatattgcgattgacgtgaaggacaag
aaaggagttaagaaagcgcaggccgacggaagtgcaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCGCCATCGAAGTTGCCAGTGGAG 676 473 355 Antisense
GCTCAACGCGAACTTATGTCGCTGC 63 303 383 Antisense
CGGAGGGAGAAGGCCCGAATCCTGA 610 241 446 Antisense
GAAATTTTCGCGCTCGTCAAAACTC 557 361 472 Antisense
TGGCTGAAGCTATTGGATCCCGCGA 469 545 575 Antisense
GATCCCGCGAGCAATTCGTGTTGGT 672 415 590 Antisense
TTTCCTCTCTACAGTTCCAAATAAA 69 671 660 Antisense
GAAGGCCCAGTCGATTGGAGATCTG 674 331 760 Antisense
AACGCTCGGGAGTGTGAGACGGCCT 82 143 799 Antisense
GAACCGACATATTGCGATTGACGTG 125 347 861 Antisense
AAGCGCAGGCCGACGGAAGTGCACA 620 149 908 Antisense
Sequence Source Affymetrix Proprietary Database