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[187938_at]

Description B0513.1 /REP_DB=WormBase Gene ID /WP=CE15557 /GEN=gei-5 /TR=O62020 /GB=CAB05113.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 62      6grp_high1_10_10 > 26      4grp_highest_8_8 > 55      4grp_high_8_8 > 10      6grp_highest_nonorm > 80      6grp_total > 26      4grp_total > 21      6grp_highest_sub > Cu      6grp_cgc > 24      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 192
1 2 3 4 range
A 353.4
(1.8)
P 		239.1
(-1.8)
P 		199.9
(-3)
P 		391.7
(3)
P 		192
B 321.2
(3)
P 		241.1
(-1)
P 		200.5
(-3)
P 		302.5
(2.1)
P 		121
C 268.2
(-0.4)
P 		248.9
(-1.5)
P 		222
(-3)
P 		327.6
(3)
P 		106
avr 314.3
(1.8) 		243
(-1.4) 		207.5
(-3) 		340.6
(3) 		133

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 192
1 2 3 4 5 6 range
A 257
(3)
P 		242.5
(2.2)
P 		170.6
(-1.8)
P 		160.9
(-2.4)
P 		149.8
(-3)
P 		223.5
(1.1)
P 		107
B 285.5
(3)
P 		248.2
(1.8)
P 		159.7
(-0.9)
P 		122.1
(-2.1)
P 		93.6
(-3)
P 		193
(0.1)
P 		192
C 267.7
(3)
P 		234
(1.7)
P 		247.9
(2.2)
P 		116
(-3)
P 		158.4
(-1.3)
P 		130
(-2.4)
P 		152
avr 270.1
(3) 		241.6
(1.8) 		192.7
(-0.4) 		133
(-3) 		133.9
(-3) 		182.2
(-0.8) 		137
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 128.7
(3)
P 		51.9
(-3)
P 		52.3
(-3)
P 		57.3
(-2.6)
P 		77
B 102.4
(3)
P 		59.5
(-1.6)
P 		46.4
(-3)
P 		64.5
(-1.1)
P 		56
C 88.8
(3)
P 		71.6
(-0.1)
P 		77.4
(1)
P 		55.2
(-3)
P 		34
avr 106.6
(3) 		61
(-2.7) 		58.7
(-3) 		59
(-3) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 76
1 2 3 4 5 6 range
A 47.4
(-1.8)
P 		86.2
(3)
P 		37.2
(-3)
P 		40.8
(-2.6)
P 		45.2
(-2)
P 		54.4
(-0.9)
P 		49
B 99.4
(3)
P 		82.6
(1.5)
P 		38.8
(-2.4)
P 		32.3
(-3)
P 		38
(-2.5)
P 		72
(0.5)
P 		67
C 99.2
(3)
P 		71.4
(0.8)
P 		63.1
(0.1)
P 		23.3
(-3)
P 		51.3
(-0.8)
P 		43.5
(-1.4)
P 		76
avr 82
(3) 		80.1
(2.8) 		46.4
(-1.3) 		32.1
(-3) 		44.8
(-1.5) 		56.6
(-0.1) 		50
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187938_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9365
Sequence Type Exemplar sequence
Representative Public ID B0513.1 NCBI
Target Description B0513.1 /REP_DB=WormBase Gene ID /WP=CE15557 /GEN=gei-5 /TR=O62020 /GB=CAB05113.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070273(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070273 NCBI Caenorhabditis elegans abnormal cell LINeage family member (lin-66) (lin-66) mRNA, complete cds. 11/11 None
SNAP00000026163 ENSEMBL cdna:SNAP chromosome:CEL140:IV:13888192:13892327:1 11/11 None
GENEFINDER00000026172 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:13888180:13892327:1 11/11 None
B0513.1 ENSEMBL cdna:known chromosome:CEL140:IV:13888058:13893950:1 gene:B0513.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13888185-13892333(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol lin-66
Ensembl B0513.1
Entrez Gene 178354 Entrez gene
SwissProt O62020 EMBL-EBI
WormBase CE15557 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05113 Hypothetical protein B0513.1 [Caenorhabditis elegans] ref|NP_502674.1| abnormal cell LINeage family member (lin-66) [Caenorhabditis elegans] 0.0
blast CAE74652 Hypothetical protein CBG22449 [Caenorhabditis briggsae] 0.0
blast CAE61435 Hypothetical protein CBG05318 [Caenorhabditis briggsae] 1.0E-97
Sequence
Target Sequence 
>C. ELEGANS:187938_AT
aatcttccctctctcaaagcggaaacagtggagaccaatggagccctctcttctccccaa
gcacctggtcgaacaacaccaacgcgaatccacgtctcacttctcttactccgttgagct
cgatgaacgagacgcttctctcgggagtcaactacaactcgttgctcgccatgccgccaa
gcgccaagaacgagagaagcattggtgttcaggctgatgactttttggatcgtatgtctt
acaatccaattggacgtaccggatgcacttgtcagtgccagtgtggaagaggtggagctg
cgatcattggaaacactcgtggatcctcttccagagcttctggaggatcggcaagcccac
caagtgctgacggtgccagccagattgatcgtttctcgccatccaatcatgccggatcaa
ttggagaccgtactcttaccgccgcggctctcggtgtcggcctagtcgaccaacagccag
cagccccaaaccacgatggtagtgctaaccaacctcagcgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATCTTCCCTCTCTCAAAGCGGAAA 309 167 1304 Antisense
TCTCTTACTCCGTTGAGCTCGATGA 330 613 1405 Antisense
GAACGAGACGCTTCTCTCGGGAGTC 411 347 1428 Antisense
GAGTCAACTACAACTCGTTGCTCGC 423 399 1448 Antisense
TGGACGTACCGGATGCACTTGTCAG 260 549 1554 Antisense
TGGAAACACTCGTGGATCCTCTTCC 430 547 1611 Antisense
GCAAGCCCACCAAGTGCTGACGGTG 294 321 1654 Antisense
TGCCAGCCAGATTGATCGTTTCTCG 350 587 1677 Antisense
TCGCCATCCAATCATGCCGGATCAA 528 599 1699 Antisense
CAATTGGAGACCGTACTCTTACCGC 9 179 1721 Antisense
TGGTAGTGCTAACCAACCTCAGCGC 556 551 1800 Antisense
Sequence Source Affymetrix Proprietary Database