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[187931_s_at]

Description F17C8.5 /REP_DB=WormBase Gene ID /WP=CE00959 /GEN=twk-5 /TR=Q19525 /GB=CAA84797.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 79      4grp_total > 04      6grp_cgc > 29      6grp_cgc_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 34.4
(2.7)
A 		1.5
(-3)
A 		2.4
(-2.8)
A 		35.9
(3)
A 		34
B 2.1
(-2.7)
A 		5.9
(1.2)
A 		1.8
(-3)
A 		7.7
(3)
A 		6
C 4.1
(-2.8)
A 		2.2
(-3)
A 		3.2
(-2.9)
A 		71.4
(3)
A 		69
avr 13.5
(-1.2) 		3.2
(-2.9) 		2.5
(-3) 		38.3
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 32.5
(3)
A 		3.3
(-2.9)
A 		19.1
(0.3)
A 		18
(0.1)
A 		2.7
(-3)
A 		29.1
(2.3)
A 		30
B 11.7
(-1.6)
A 		2
(-3)
A 		3
(-2.9)
A 		2.5
(-2.9)
A 		44.1
(3)
A 		24.7
(0.2)
A 		42
C 2.4
(-3)
A 		43.1
(3)
A 		4.5
(-2.7)
A 		12.1
(-1.6)
A 		9.7
(-1.9)
A 		37.9
(2.2)
A 		41
avr 15.5
(-1.2) 		16.1
(-1) 		8.9
(-3) 		10.9
(-2.4) 		18.8
(-0.3) 		30.6
(3) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 12.5
(3)
A 		0.3
(-3)
A 		0.6
(-2.9)
A 		5.2
(-0.6)
A 		12
B 0.7
(-1.5)
A 		1.5
(2.5)
A 		0.4
(-3)
A 		1.6
(3)
A 		1
C 1.4
(-2.6)
A 		0.6
(-3)
A 		1.1
(-2.7)
A 		12
(3)
A 		11
avr 4.9
(1.5) 		0.8
(-2.9) 		0.7
(-3) 		6.3
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 6
(2)
A 		1.2
(-2.6)
A 		4.2
(0.2)
A 		4.6
(0.6)
A 		0.8
(-3)
A 		7.1
(3)
A 		6
B 4.1
(-1.8)
A 		0.7
(-3)
A 		0.7
(-3)
A 		0.6
(-3)
A 		17.9
(3)
A 		9.2
(-0)
A 		17
C 0.9
(-3)
A 		13.2
(3)
A 		1.1
(-2.9)
A 		2.4
(-2.3)
A 		3.2
(-1.9)
A 		12.7
(2.8)
A 		12
avr 3.7
(-1.7) 		5
(-0.7) 		2
(-3) 		2.5
(-2.6) 		7.3
(1.1) 		9.7
(3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187931_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6806
Sequence Type Exemplar sequence
Representative Public ID F17C8.5 NCBI
Target Description F17C8.5 /REP_DB=WormBase Gene ID /WP=CE00959 /GEN=twk-5 /TR=Q19525 /GB=CAA84797.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065654(11), NM_065572(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065654 NCBI Caenorhabditis elegans C27F2.6 (C27F2.6) mRNA, complete cds. 11/11 A 
NM_065572 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-6) (twk-6) mRNA, complete cds. 11/11 A 
SNAP00000012148 ENSEMBL cdna:SNAP chromosome:CEL140:III:4742930:4744611:1 11/11 None
GENEFINDER00000012158 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4742930:4744611:1 11/11 None
F17C8.5 ENSEMBL cdna:known chromosome:CEL140:III:4742930:4744611:1 gene:F17C8.5 11/11 A 
C27F2.6 ENSEMBL cdna:known chromosome:CEL140:III:4979757:4980379:-1 gene:C27F2.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4742929-4744611(+) 100.0 100.0
Public Domain and Genome References
Gene Title

Gene Symbol twk-6
Ensembl F17C8.5
C27F2.6
Entrez Gene 182966 Entrez gene
192070 Entrez gene
SwissProt Q18261 EMBL-EBI
Q19525 EMBL-EBI
WormBase CE01173 Wormbase
CE00959 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5267 potassium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA84797 Hypothetical protein F17C8.5 [Caenorhabditis elegans] ref|NP_497973.1| TWiK family of potassium channels family member (twk-6) [Caenorhabditis elegans] 1.0E-165
blast NP_498055 C27F2.6 [Caenorhabditis elegans] gb|AAA81426.1| Hypothetical protein C27F2.6 [Caenorhabditis elegans] 4.0E-75
blast CAA84797 Hypothetical protein F17C8.5 [Caenorhabditis elegans] ref|NP_497973.1| TWiK family of potassium channels family member (twk-6) [Caenorhabditis elegans] 1.0E-58
blast NP_498055 C27F2.6 [Caenorhabditis elegans] gb|AAA81426.1| Hypothetical protein C27F2.6 [Caenorhabditis elegans] 5.0E-58
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498055.1 3 20-39,46-68,72-94
NP_497973.1 6 36-58,113-135,140-162,201-223,228-250,254-276
F17C8.5 6 36-58,113-135,140-162,201-223,228-250,254-276
C27F2.6 3 20-39,46-68,72-94
SNAP00000012148 6 36-58,113-135,140-162,201-223,228-250,254-276
GENEFINDER00000012158 6 36-58,113-135,140-162,201-223,228-250,254-276
Sequence
Target Sequence 
>C. ELEGANS:187931_S_AT
ttctctgctcaatatctcttctgagttccagtgcacttttctcgtcaattgagaatattt
catatctcagttccgtttatttcgggattatcacaatgtttttgattggaatcggagata
tcgttcctacaaatttagtatggttcagtggatattgtatgttattcttgatttctgatg
ttctgtcaaatcagattttctacttttgtcaggcaagggttcgatacttcttccatattc
tcgctcggaaaattttgttattgagagaagaagatgacggatttcaattggaaactacag
tatccctacaacatattccaattattaacagccaatgcatgcctagcttggttctggact
gcgaaaagga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCTCTGCTCAATATCTCTTCTGAG 190 693 638 Antisense
ATATCTCTTCTGAGTTCCAGTGCAC 140 21 649 Antisense
TCTTCTGAGTTCCAGTGCACTTTTC 361 617 654 Antisense
GTTCCTACAAATTTAGTATGGTTCA 236 441 760 Antisense
AGGCAAGGGTTCGATACTTCTTCCA 26 53 848 Antisense
TCGATACTTCTTCCATATTCTCGCT 397 603 858 Antisense
TTCAATTGGAAACTACAGTATCCCT 422 681 920 Antisense
AACATATTCCAATTATTAACAGCCA 81 139 947 Antisense
TATTAACAGCCAATGCATGCCTAGC 660 661 960 Antisense
CATGCCTAGCTTGGTTCTGGACTGC 649 209 975 Antisense
GCTTGGTTCTGGACTGCGAAAAGGA 212 307 983 Antisense
Sequence Source Affymetrix Proprietary Database