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[187930_at]

Description F17C8.5 /REP_DB=WormBase Gene ID /WP=CE00959 /GEN=twk-5 /TR=Q19525 /GB=CAA84797.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 47      4grp_high_8_8 > 29      6grp_total > 75      4grp_total > 19      6grp_cgc > 37      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 23.8
(-3)
A 		26.7
(-2.5)
A 		61
(3)
P 		45
(0.4)
A 		37
B 55.6
(3)
P 		38.6
(0.6)
A 		13.2
(-3)
A 		39.7
(0.8)
P 		42
C 35.4
(-1)
A 		70.8
(1.1)
P 		2.9
(-3)
A 		101.8
(3)
P 		99
avr 38.3
(-0.9) 		45.4
(0.2) 		25.7
(-3) 		62.2
(3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 48.6
(1.8)
P 		15.2
(-3)
A 		43.2
(1.1)
P 		28.1
(-1.1)
A 		36.1
(0)
P 		56.6
(3)
P 		41
B 29.3
(-0.4)
A 		25
(-1.7)
A 		20.8
(-3)
A 		37.3
(2.1)
P 		35.1
(1.4)
P 		40.3
(3)
P 		20
C 18.2
(-1.7)
A 		44.4
(1.3)
P 		29.9
(-0.3)
A 		59.2
(3)
A 		6.6
(-3)
A 		31.2
(-0.2)
A 		53
avr 32
(-0.8) 		28.2
(-2.2) 		31.3
(-1.1) 		41.5
(2.6) 		25.9
(-3) 		42.7
(3) 		17
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 8.7
(-1.3)
A 		5.8
(-3)
A 		15.9
(3)
P 		6.6
(-2.5)
A 		10
B 17.7
(3)
P 		9.5
(-0.4)
A 		3.1
(-3)
A 		8.5
(-0.8)
P 		15
C 11.7
(0.3)
A 		20.4
(3)
P 		1
(-3)
A 		17.2
(2)
P 		19
avr 12.7
(3) 		11.9
(2.2) 		6.7
(-3) 		10.8
(1.1) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 9
(-0.4)
P 		5.4
(-3)
A 		9.4
(-0.1)
P 		7.1
(-1.8)
A 		10.9
(0.9)
P 		13.8
(3)
P 		8
B 10.2
(0.1)
A 		8.3
(-1)
A 		5
(-3)
A 		9.9
(-0.1)
P 		14.3
(2.6)
P 		15
(3)
P 		10
C 6.8
(-0.5)
A 		13.5
(3)
P 		7.6
(-0.1)
A 		11.9
(2.2)
A 		2.1
(-3)
A 		10.5
(1.4)
A 		11
avr 8.7
(-1.6) 		9.1
(-1.1) 		7.3
(-3) 		9.6
(-0.6) 		9.1
(-1.1) 		13.1
(3) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187930_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6806
Sequence Type Exemplar sequence
Representative Public ID F17C8.5 NCBI
Target Description F17C8.5 /REP_DB=WormBase Gene ID /WP=CE00959 /GEN=twk-5 /TR=Q19525 /GB=CAA84797.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065572(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065572 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-6) (twk-6) mRNA, complete cds. 11/11 A 
SNAP00000012148 ENSEMBL cdna:SNAP chromosome:CEL140:III:4742930:4744611:1 11/11 None
GENEFINDER00000012158 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4742930:4744611:1 11/11 None
F17C8.5 ENSEMBL cdna:known chromosome:CEL140:III:4742930:4744611:1 gene:F17C8.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4742929-4744611(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol twk-6
Ensembl F17C8.5
Entrez Gene 192070 Entrez gene
SwissProt Q19525 EMBL-EBI
WormBase CE00959 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5267 potassium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA84797 Hypothetical protein F17C8.5 [Caenorhabditis elegans] ref|NP_497973.1| TWiK family of potassium channels family member (twk-6) [Caenorhabditis elegans] 1.0E-165
blast NP_498055 C27F2.6 [Caenorhabditis elegans] gb|AAA81426.1| Hypothetical protein C27F2.6 [Caenorhabditis elegans] 5.0E-58
blast CAE71315 Hypothetical protein CBG18207 [Caenorhabditis briggsae] 5.0E-32
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497973.1 6 36-58,113-135,140-162,201-223,228-250,254-276
F17C8.5 6 36-58,113-135,140-162,201-223,228-250,254-276
SNAP00000012148 6 36-58,113-135,140-162,201-223,228-250,254-276
GENEFINDER00000012158 6 36-58,113-135,140-162,201-223,228-250,254-276
Sequence
Target Sequence 
>C. ELEGANS:187930_AT
atgattccagtttttattgcttttaagtttgaattcggaacatttttggctcattttctt
gttgtcgtgtcgaatcggactcgacttgcagtgaagaaagcgtactacaaattgtcgcaa
aaccccgagaacgctgaaactccgagtaattcgctccaacatgattatttgatatttcta
tcttcacttcttctctgctcaatatctcttctgagttccagtgcacttttctcgtcaatt
gagaatatttcatatctcagttccgtttatttcgggattatcacaatgtttttgattgga
atcggagatatcgttcctacaaatttagtatggttcagtggatattgtatgttattcttg
atttctgatgttctgtcaaatcagattttctacttttgtcaggcaagggttcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGATTCCAGTTTTTATTGCTTTTA 1 47 448 Antisense
TTTTGGCTCATTTTCTTGTTGTCGT 283 679 491 Antisense
CTTGTTGTCGTGTCGAATCGGACTC 565 217 505 Antisense
GAATCGGACTCGACTTGCAGTGAAG 572 327 519 Antisense
TACAAATTGTCGCAAAACCCCGAGA 465 625 553 Antisense
ACCCCGAGAACGCTGAAACTCCGAG 173 87 569 Antisense
AACTCCGAGTAATTCGCTCCAACAT 179 141 585 Antisense
ATATTTCATATCTCAGTTCCGTTTA 169 19 692 Antisense
GAATCGGAGATATCGTTCCTACAAA 112 329 746 Antisense
TATTCTTGATTTCTGATGTTCTGTC 541 663 800 Antisense
TCTACTTTTGTCAGGCAAGGGTTCG 177 613 836 Antisense
Sequence Source Affymetrix Proprietary Database