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[187910_at]

Description W08D2.4 /REP_DB=WormBase Gene ID /WP=CE25153 /GEN=fat-3 /TR=Q23221 /GB=CAA94233.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 28      6grp_high1_10_10 > 82      4grp_highest_8_8 > 42      4grp_high_8_8 > 41      6grp_highest_nonorm > 29      6grp_total > 70      4grp_total > 54      6grp_highest_sub > 04      6grp_cgc > 85      6grp_high1_sub > 04      6grp_cgc_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8529
1 2 3 4 range
A 9564.2
(3)
P 		5564.4
(-0.8)
P 		5370.3
(-1)
P 		3238.1
(-3)
P 		6326
B 10915.8
(3)
P 		5552.8
(-0.8)
P 		5933.8
(-0.5)
P 		2386.5
(-3)
P 		8529
C 9196.1
(3)
P 		5390
(-0.6)
P 		5288.6
(-0.7)
P 		2792.2
(-3)
P 		6404
avr 9892
(3) 		5502.4
(-0.7) 		5530.9
(-0.7) 		2805.6
(-3) 		7086

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13109
1 2 3 4 5 6 range
A 12322.4
(-1.3)
P 		9835.6
(-3)
P 		14516.9
(0.3)
P 		18459.8
(3)
P 		15358.4
(0.8)
P 		11424.6
(-1.9)
P 		8624
B 9864.5
(-3)
P 		10217.6
(-2.7)
P 		11886.6
(-1.5)
P 		17727.8
(3)
P 		13169.4
(-0.5)
P 		10427.1
(-2.6)
P 		7863
C 8059.3
(-3)
P 		10494.8
(-1.9)
P 		14051.3
(-0.3)
P 		21168.1
(3)
P 		14097.3
(-0.2)
P 		9258.7
(-2.5)
P 		13109
avr 10082.1
(-3) 		10182.7
(-2.9) 		13484.9
(-0.7) 		19118.6
(3) 		14208.4
(-0.3) 		10370.1
(-2.8) 		9037
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3012
1 2 3 4 range
A 3482.3
(3)
P 		1207.9
(-1.5)
P 		1403.9
(-1.1)
P 		474
(-3)
P 		3008
B 3481
(3)
P 		1371.4
(-1.3)
P 		1375
(-1.3)
P 		508.6
(-3)
P 		2972
C 3044.2
(3)
P 		1549.7
(-0.5)
P 		1843.3
(0.2)
P 		470.8
(-3)
P 		2573
avr 3335.8
(3) 		1376.3
(-1.1) 		1540.7
(-0.8) 		484.5
(-3) 		2851

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3079
1 2 3 4 5 6 range
A 2271.5
(-3)
P 		3498
(0.1)
P 		3161.5
(-0.8)
P 		4681.8
(3)
P 		4634.5
(2.9)
P 		2781.5
(-1.7)
P 		2410
B 3432.4
(-1.7)
P 		3401
(-1.7)
P 		2886.4
(-3)
P 		4695.5
(1.4)
P 		5350.6
(3)
P 		3888.2
(-0.6)
P 		2464
C 2987.2
(-3)
P 		3202.3
(-2.2)
P 		3576.3
(-0.8)
P 		4256.9
(1.8)
P 		4561.7
(3)
P 		3099.4
(-2.6)
P 		1575
avr 2897
(-3) 		3367.1
(-1.6) 		3208.1
(-2) 		4544.7
(2.1) 		4848.9
(3) 		3256.4
(-1.9) 		1952
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187910_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10219
Sequence Type Exemplar sequence
Representative Public ID W08D2.4 NCBI
Target Description W08D2.4 /REP_DB=WormBase Gene ID /WP=CE25153 /GEN=fat-3 /TR=Q23221 /GB=CAA94233.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069351(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069351 NCBI Caenorhabditis elegans FATty acid desaturase family member (fat-3) (fat-3) mRNA, complete cds. 11/11 None
SNAP00000011768 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9803037:9805973:1 11/11 None
GENEFINDER00000011778 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9803037:9805973:1 11/11 None
W08D2.4 ENSEMBL cdna:known chromosome:CEL140:IV:9803011:9806095:1 gene:W08D2.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9803042-9805979(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol fat-3
Ensembl W08D2.4
Entrez Gene 177820 Entrez gene
SwissProt Q23221 EMBL-EBI
WormBase CE25153 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1584945_AT similar to fatty acid desaturase 3 cfa
CANINE_2:CFA.3957.1.A1_AT similar to fatty acid desaturase 3 cfa
CANINE_2:CFAAFFX.24504.1.S1_S_AT similar to fatty acid desaturase 3 cfa
CANINE_2:CFAAFFX.24504.1.S1_X_AT similar to fatty acid desaturase 3 cfa
HG-U133_PLUS_2:204257_AT fatty acid desaturase 3 hs
HG-U133A:204257_AT fatty acid desaturase 3 hs
HG-FOCUS:204257_AT fatty acid desaturase 3 hs
HG-U133A_2:204257_AT fatty acid desaturase 3 hs
HU35KSUBA:H06532_AT fatty acid desaturase 3 hs
HU35KSUBA:RC_AA279632_AT fatty acid desaturase 3 hs
HG-U133_PLUS_2:216080_S_AT fatty acid desaturase 3 hs
HG-U133A_2:216080_S_AT fatty acid desaturase 3 hs
HG-U133A:216080_S_AT fatty acid desaturase 3 hs
U133_X3P:HS.21765.1.S1_3P_A_AT fatty acid desaturase 3 hs
HG-U95AV2:34224_AT fatty acid desaturase 3 hs
HG-U95B:43394_S_AT fatty acid desaturase 3 hs
MOUSE430A_2:1418773_AT fatty acid desaturase 3 mm
MOE430A:1418773_AT fatty acid desaturase 3 mm
MOUSE430_2:1418773_AT fatty acid desaturase 3 mm
MG-U74CV2:168721_R_AT fatty acid desaturase 3 mm
MG-U74CV2:167028_AT fatty acid desaturase 3 mm
MOE430A:1449219_AT fatty acid desaturase 3 mm
MOUSE430A_2:1449219_AT fatty acid desaturase 3 mm
MOUSE430_2:1449219_AT fatty acid desaturase 3 mm
MG-U74BV2:111391_AT Fatty acid desaturase 3 (Fads3), mRNA mm
MG-U74BV2:165219_S_AT Fatty acid desaturase 3 (Fads3), mRNA mm
MOE430B:1435910_AT Fatty acid desaturase 3 (Fads3), mRNA mm
MOUSE430_2:1435910_AT Fatty acid desaturase 3 (Fads3), mRNA mm
MU19KSUBB:TC26197_AT Fatty acid desaturase 3 (Fads3), mRNA mm
MU19KSUBB:TC26197_G_AT Fatty acid desaturase 3 (Fads3), mRNA mm
MU19KSUBB:TC31797_AT Fatty acid desaturase 3 (Fads3), mRNA mm
MU19KSUBC:TC41817_AT Fatty acid desaturase 3 (Fads3), mRNA mm
MU19KSUBC:TC42083_AT Fatty acid desaturase 3 (Fads3), mRNA mm
RG-U34C:RC_AI178762_AT fatty acid desaturase 3 rn
RAE230A:1372476_AT fatty acid desaturase 3 rn
RAT230_2:1372476_AT fatty acid desaturase 3 rn
RG-U34B:RC_AA956329_AT Fatty acid desaturase 3 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5215 transporter activity inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94233 Hypothetical protein W08D2.4 [Caenorhabditis elegans] ref|NP_501752.1| FATty acid desaturase family member (fat-3) [Caenorhabditis elegans] 0.0
blast AAC15586 delta6-fatty-acid-desaturase [Caenorhabditis elegans] 0.0
blast CAE59986 Hypothetical protein CBG03479 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005804 EMBL-EBI Fatty acid desaturase 2.1E-65
Pfam IPR001199 EMBL-EBI Cytochrome b5 3.2E-5
Pfam IPR005804 EMBL-EBI Fatty acid desaturase 2.1E-65
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501752.1 3 136-158,289-311,316-338
W08D2.4 3 136-158,289-311,316-338
SNAP00000011768 3 166-188,319-341,346-368
GENEFINDER00000011778 3 166-188,319-341,346-368
Sequence
Target Sequence 
>C. ELEGANS:187910_AT
aacatctctatttcaccgcaatgcttccaatgctccgtttctcatggactggtcagtcag
ttcaatgggtattcaaagagaatcaaatggagtacaaggtctatcaaagaaatgcattct
gggagcaagcaacaattgttggacattgggcttgggtattctatcaattgttcttattac
caacatggccacttcgggttgcttatttcattatttcacaaatgggaggaggccttttga
ttgctcacgtagtcactttcaaccataactctgttgataagtatccagccaattctcgaa
ttttaaacaacttcgccgctcttcaaattttgaccacacgcaacatgactccatctccat
tcattgattggctttggggtggactcaattatcagatcgagcaccacttgttcccaacaa
tgccacgttgcaatctgaatgcttgcatgaaatatgtgaaagaatggtgcaaagagaata
atcttccttacctcgtcgatgactactt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACATCTCTATTTCACCGCAATGCT 506 137 767 Antisense
TTCCAATGCTCCGTTTCTCATGGAC 644 701 791 Antisense
GGACATTGGGCTTGGGTATTCTATC 554 525 907 Antisense
TACCAACATGGCCACTTCGGGTTGC 459 647 944 Antisense
GGGAGGAGGCCTTTTGATTGCTCAC 10 497 990 Antisense
GATTGCTCACGTAGTCACTTTCAAC 688 433 1005 Antisense
GATAAGTATCCAGCCAATTCTCGAA 208 423 1042 Antisense
TTCGCCGCTCTTCAAATTTTGACCA 485 695 1078 Antisense
TTTTGACCACACGCAACATGACTCC 160 675 1094 Antisense
TTATCAGATCGAGCACCACTTGTTC 99 681 1155 Antisense
TTCCTTACCTCGTCGATGACTACTT 250 701 1250 Antisense
Sequence Source Affymetrix Proprietary Database