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[187903_at]

Description F31F6.4 /REP_DB=WormBase Gene ID /WP=CE05776 /GEN=flp-8 /TR=Q19940 /GB=CAA93746.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 22      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_highest_nonorm > 23      6grp_total > 22      4grp_total > 04      6grp_highest_sub > 02      6grp_cgc > 62      6grp_high1_sub > De      6grp_cgc_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5586
1 2 3 4 range
A 2033.1
(-1.8)
P 		1050.1
(-2.9)
P 		929.7
(-3)
P 		6447.9
(3)
P 		5518
B 2043.8
(-1.5)
P 		1170.1
(-2.6)
P 		861.5
(-3)
P 		5508.8
(3)
P 		4647
C 1959
(-1.8)
P 		921.7
(-3)
P 		1031.1
(-2.9)
P 		6067.8
(3)
P 		5146
avr 2012
(-1.7) 		1047.3
(-2.9) 		940.8
(-3) 		6008.2
(3) 		5067

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2558
1 2 3 4 5 6 range
A 2039.8
(0.4)
P 		2577.7
(2.2)
P 		2839.7
(3)
P 		2272.5
(1.2)
P 		1539.5
(-1.2)
P 		986.6
(-3)
P 		1853
B 2641.5
(1.1)
P 		3460.9
(3)
P 		3359.6
(2.8)
P 		2223.3
(0.1)
P 		2093.3
(-0.2)
P 		903.2
(-3)
P 		2558
C 2645.8
(1.4)
P 		3238.4
(3)
P 		1821.5
(-0.8)
P 		1944.2
(-0.5)
P 		1695.5
(-1.2)
P 		1020.1
(-3)
P 		2218
avr 2442.4
(1.2) 		3092.3
(3) 		2673.6
(1.8) 		2146.7
(0.3) 		1776.1
(-0.7) 		970
(-3) 		2122
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 974
1 2 3 4 range
A 740.2
(1.3)
P 		227.9
(-3)
P 		243.1
(-2.9)
P 		943.8
(3)
P 		716
B 651.7
(-0.2)
P 		289
(-2.4)
P 		199.6
(-3)
P 		1173.9
(3)
P 		974
C 648.5
(0)
P 		265
(-3)
P 		359.4
(-2.3)
P 		1023.1
(3)
P 		758
avr 680.1
(0.2) 		260.6
(-3) 		267.4
(-2.9) 		1046.9
(3) 		786

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 912
1 2 3 4 5 6 range
A 376
(-1.8)
P 		916.8
(3)
P 		618.4
(0.4)
P 		576.4
(-0)
P 		464.6
(-1)
P 		240.2
(-3)
P 		677
B 919.1
(1.3)
P 		1152
(3)
P 		815.8
(0.5)
P 		588.9
(-1.1)
P 		850.5
(0.8)
P 		336.8
(-3)
P 		815
C 980.7
(2.9)
P 		988.1
(3)
P 		463.6
(-1.9)
P 		391
(-2.5)
P 		548.6
(-1.1)
P 		341.5
(-3)
P 		647
avr 758.6
(0.8) 		1019
(3) 		632.6
(-0.3) 		518.8
(-1.2) 		621.2
(-0.3) 		306.2
(-3) 		713
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187903_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17511
Sequence Type Exemplar sequence
Representative Public ID F31F6.4 NCBI
Target Description F31F6.4 /REP_DB=WormBase Gene ID /WP=CE05776 /GEN=flp-8 /TR=Q19940 /GB=CAA93746.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171804(11), NM_171805(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171804 NCBI Caenorhabditis elegans FMRF-Like Peptide family member (flp-8) (flp-8) mRNA, complete cds. 11/11 None
NM_171805 NCBI Caenorhabditis elegans FMRF-Like Peptide family member (flp-8) (flp-8) mRNA, complete cds. 11/11 None
SNAP00000015867 ENSEMBL cdna:SNAP chromosome:CEL140:X:14881455:14881984:1 11/11 None
GENEFINDER00000015876 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:14881455:14894058:1 11/11 None
F31F6.4b ENSEMBL cdna:known chromosome:CEL140:X:14881414:14882217:1 gene:F31F6.4 11/11 A 
F31F6.4a ENSEMBL cdna:known chromosome:CEL140:X:14881455:14881984:1 gene:F31F6.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:14881454-14881984(+) 100.0 100.0
Public Domain and Genome References
Ensembl F31F6.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA93751 Hypothetical protein F31F6.5 [Caenorhabditis elegans] 0.0
blast NP_510472 abnormal DAuer Formation family member (daf-6) [Caenorhabditis elegans] 0.0
blast CAE63242 Hypothetical protein CBG07607 [Caenorhabditis briggsae] 0.0
blast CAA93746 Hypothetical protein F31F6.4a [Caenorhabditis elegans] ref|NP_741935.1| FMRF-Like Peptide family member (flp-8) [Caenorhabditis elegans] gb|AAC08945.1| FMRFamide-like peptide 8 [Caenorhabditis elegans] 2.0E-71
blast CAD21627 Hypothetical protein F31F6.4b [Caenorhabditis elegans] ref|NP_741934.1| FMRF-Like Peptide family member (flp-8) [Caenorhabditis elegans] 2.0E-70
blast CAA93746 Hypothetical protein F31F6.4a [Caenorhabditis elegans] ref|NP_741935.1| FMRF-Like Peptide family member (flp-8) [Caenorhabditis elegans] gb|AAC08945.1| FMRFamide-like peptide 8 [Caenorhabditis elegans] 1.0E-63
blast CAD21627 Hypothetical protein F31F6.4b [Caenorhabditis elegans] ref|NP_741934.1| FMRF-Like Peptide family member (flp-8) [Caenorhabditis elegans] 1.0E-63
Protein Domains
Database ID Description E-Value
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0068
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.0068
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.099
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.57
Pfam IPR003392 EMBL-EBI Patched family 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000015876 12 199-221,441-460,480-502,507-529,549-571,578-600,723-745,922-944,954-976,983-1005,1020-1042,1054-1076
Sequence
Target Sequence 
>C. ELEGANS:187903_AT
ttttttctatttttgtactcgccatatcggccaacgctagctgtgatgtgtccgcactga
ccaccgagaatgagaaggaactcggactccggatttgccatcttgaggcggagatgcaag
tcgtccaaagagccttgcaggaggtgatgcagcagacagatgtcacactgtacgatcaag
aagttccggtgatgaacaagcgtaaaaacgagtttatccgatttggaaaacgcagtgacg
gaatggaaaaacgaaagaacgagttcatccggtttggaaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTTTTCTATTTTTGTACTCGCCAT 643 675 29 Antisense
TTTTGTACTCGCCATATCGGCCAAC 41 677 39 Antisense
GGCCAACGCTAGCTGTGATGTGTCC 395 543 57 Antisense
CCGCACTGACCACCGAGAATGAGAA 312 261 80 Antisense
ATGAGAAGGAACTCGGACTCCGGAT 334 25 98 Antisense
GACTCCGGATTTGCCATCTTGAGGC 699 373 113 Antisense
GAGGCGGAGATGCAAGTCGTCCAAA 439 403 133 Antisense
GTCGTCCAAAGAGCCTTGCAGGAGG 183 473 148 Antisense
GGAGGTGATGCAGCAGACAGATGTC 240 517 168 Antisense
CAGATGTCACACTGTACGATCAAGA 96 207 185 Antisense
GAACGAGTTCATCCGGTTTGGAAAG 408 327 285 Antisense
Sequence Source Affymetrix Proprietary Database