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[187887_at]

Description Y5H2A.A_rc /REP_DB=WormBase Gene ID /WP=CE21311 /CHR=5 /FEA=Sanger Annotation /REVCOMP /DEF=(ST.LOUIS) protein_id:AAF59624.1
Cluster 6grp_total > 81      4grp_total > 26      6grp_cgc > 86      6grp_cgc_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 12.3
(1.8)
A 		12.2
(1.8)
A 		4.1
(-3)
A 		14.3
(3)
A 		10
B 3.3
(-2.5)
A 		23.3
(3)
A 		17.7
(1.5)
A 		1.3
(-3)
A 		22
C 4.7
(-2.7)
A 		4.2
(-2.8)
A 		2.8
(-3)
A 		39.6
(3)
A 		37
avr 6.8
(-3) 		13.2
(0.3) 		8.2
(-2.3) 		18.4
(3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 1.7
(-2.6)
A 		0.8
(-3)
A 		9.7
(1.2)
A 		13.4
(3)
A 		9
(0.9)
A 		3.9
(-1.5)
A 		13
B 1
(-3)
A 		10.2
(2.4)
A 		4.5
(-1)
A 		11.3
(3)
A 		6.7
(0.3)
A 		2
(-2.4)
A 		10
C 11.8
(2.4)
A 		1.8
(-3)
A 		3.8
(-1.9)
A 		6.1
(-0.7)
A 		13
(3)
A 		2.3
(-2.7)
A 		11
avr 4.8
(-1.3) 		4.3
(-1.7) 		6
(-0.4) 		10.3
(3) 		9.6
(2.4) 		2.7
(-3) 		8
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6
1 2 3 4 range
A 4.5
(3)
A 		2.7
(-0.2)
A 		1.1
(-3)
A 		2.1
(-1.2)
A 		3
B 1.1
(-2.1)
A 		5.8
(3)
A 		4.1
(1.1)
A 		0.3
(-3)
A 		6
C 1.6
(-2.4)
A 		1.2
(-2.8)
A 		1
(-3)
A 		6.7
(3)
A 		6
avr 2.4
(-1.4) 		3.2
(3) 		2.1
(-3) 		3
(1.9) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4
1 2 3 4 5 6 range
A 0.3
(-3)
A 		0.3
(-3)
A 		2.1
(0.5)
A 		3.4
(3)
A 		2.7
(1.6)
A 		0.9
(-1.8)
A 		3
B 0.3
(-3)
A 		3.4
(3)
A 		1.1
(-1.5)
A 		3
(2.2)
A 		2.7
(1.6)
A 		0.7
(-2.2)
A 		3
C 4.4
(3)
A 		0.5
(-3)
A 		1
(-2.2)
A 		1.2
(-1.9)
A 		4.2
(2.7)
A 		0.8
(-2.5)
A 		4
avr 1.7
(-0.8) 		1.4
(-1.5) 		1.4
(-1.5) 		2.5
(1.3) 		3.2
(3) 		0.8
(-3) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187887_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.28201
Sequence Type Exemplar sequence
Representative Public ID Y5H2A.A NCBI
Target Description Y5H2A.A_rc /REP_DB=WormBase Gene ID /WP=CE21311 /CHR=5 /FEA=Sanger Annotation /REVCOMP /DEF=(ST.LOUIS) protein_id:AAF59624.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182410
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
Y5H2A.3 ENSEMBL cdna:known chromosome:CEL140:V:2274710:2275805:1 gene:Y5H2A.3 A 
SNAP00000022368 ENSEMBL cdna:SNAP chromosome:CEL140:V:2274532:2275791:1 None
NM_182410 NCBI Caenorhabditis elegans Activated in Blocked Unfolded protein response family member (abu-4) (abu-4) mRNA, complete cds. A 
GENEFINDER00000022372 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2274710:2275805:1 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2272268-2275065(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol abu-4
Ensembl Y5H2A.3
Entrez Gene 353458 Entrez gene
SwissProt Q86S74 EMBL-EBI
WormBase CE21313 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_872210 Activated in Blocked Unfolded protein response family member (abu-4) [Caenorhabditis elegans] gb|AAO25980.1| Activated in blocked unfolded protein response protein 4 [Caenorhabditis elegans] 3.0E-43
blast CAB54985 Hypothetical protein Y105C5A.6 [Caenorhabditis elegans] emb|CAB54982.1| Hypothetical protein Y105C5A.3 [Caenorhabditis elegans] ref|NP_502875.1| Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-79) [Caenorhabditis elegans] ref|NP_502872.1| Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-76) [Caenorhabditis elegans] 2.0E-35
blast CAB54984 Hypothetical protein Y105C5A.5 [Caenorhabditis elegans] ref|NP_502874.1| Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-78) [Caenorhabditis elegans] 4.0E-35
Protein Domains
Database ID Description E-Value
Pfam IPR009475 EMBL-EBI Protein of unknown function DUF1096 3.1E-33
Pfam IPR009475 EMBL-EBI Protein of unknown function DUF1096 3.1E-33
Sequence
Target Sequence 
>C. ELEGANS:187887_AT
gcacagccacatgattgacgtttatcgcgaattgatgttgcctttgcaactttccgtgtt
ccgaagatatagtgtatacgactatgagaggatatatctgtgttctttcgttcatcagca
gccatgccatgtttgcgttcatcatgaagccggatgaatggatggagtttgataagtggg
aagaaaatctccggtatgtgtttgatggctgatgaaaaaaggttggagtacgttggtata
aaagtctgaaaaattgagaaacggacatcagttgcttctcgttgcagctcttcgaacgga
ttaacaatgcgctttatcgcaattgcagctcttcttgcttctagctttctacttattgag
gtacgtattctttttccaggcaacggcaacat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCACAGCCACATGATTGACGTTTAT 421 317 1538 Antisense
ATCGCGAATTGATGTTGCCTTTGCA 621 35 1561 Antisense
TTTGCAACTTTCCGTGTTCCGAAGA 413 667 1580 Antisense
TATATCTGTGTTCTTTCGTTCATCA 597 661 1630 Antisense
AGCAGCCATGCCATGTTTGCGTTCA 243 77 1654 Antisense
CTCCGGTATGTGTTTGATGGCTGAT 246 235 1726 Antisense
GAAACGGACATCAGTTGCTTCTCGT 700 357 1795 Antisense
TTCTCGTTGCAGCTCTTCGAACGGA 55 691 1813 Antisense
ACGGATTAACAATGCGCTTTATCGC 24 97 1833 Antisense
GAGGTACGTATTCTTTTTCCAGGCA 561 241 1895 Antisense
TTCTTTTTCCAGGCAACGGCAACAT 40 687 1905 Antisense
Sequence Source Affymetrix Proprietary Database