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[187883_at]

Description M4.2 /REP_DB=WormBase Gene ID /WP=CE17188 /GEN=puf-4 /TR=Q9TYX0 /GB=AAC68995.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 0      4grp_total > 59      6grp_total_sub > Hat      6grp_cgc > 53      6grp_cgc_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 61
1 2 3 4 range
A 39.6
(0.3)
A 		66.5
(3)
A 		14.3
(-2.3)
A 		7.7
(-3)
A 		59
B 36.9
(-1.6)
A 		33.5
(-2.2)
A 		61.9
(3)
A 		29.4
(-3)
A 		33
C 10.7
(-0.8)
A 		10.1
(-1)
A 		19.4
(3)
A 		5.6
(-3)
A 		14
avr 29.1
(1) 		36.7
(3) 		31.9
(1.7) 		14.2
(-3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 2.9
(-3)
A 		20.1
(2.4)
A 		22.1
(3)
A 		7.5
(-1.6)
A 		4.9
(-2.4)
A 		9.2
(-1)
A 		19
B 15.8
(-0.6)
A 		19
(0.1)
A 		4.3
(-3)
A 		33.2
(3)
A 		14.5
(-0.9)
A 		7.1
(-2.4)
A 		29
C 19.4
(-0.3)
A 		35.3
(3)
A 		6.1
(-3)
A 		10
(-2.2)
A 		9.7
(-2.3)
A 		20.9
(0)
A 		29
avr 12.7
(-1.8) 		24.8
(3) 		10.8
(-2.6) 		16.9
(-0.1) 		9.7
(-3) 		12.4
(-1.9) 		15
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 14.4
(3)
A 		14.4
(3)
A 		3.7
(-1.8)
A 		1.1
(-3)
A 		13
B 11.8
(1.1)
A 		8.3
(-1.5)
A 		14.4
(3)
A 		6.3
(-3)
A 		8
C 3.5
(-0.4)
A 		2.9
(-1)
A 		6.8
(3)
A 		0.9
(-3)
A 		6
avr 9.9
(3) 		8.5
(1.8) 		8.3
(1.6) 		2.8
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 0.5
(-3)
A 		7.1
(3)
A 		4.8
(0.9)
A 		1.9
(-1.7)
A 		1.5
(-2.1)
A 		2.2
(-1.5)
A 		7
B 5.5
(0.5)
A 		6.3
(1.1)
A 		1
(-3)
A 		8.8
(3)
A 		5.9
(0.8)
A 		2.6
(-1.8)
A 		8
C 7.2
(0.7)
A 		10.8
(3)
A 		1.6
(-3)
A 		2
(-2.7)
A 		3.1
(-2)
A 		7
(0.5)
A 		9
avr 4.4
(-1) 		8.1
(3) 		2.5
(-3) 		4.2
(-1.2) 		3.5
(-1.9) 		3.9
(-1.5) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187883_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11243
Sequence Type Exemplar sequence
Representative Public ID M4.2 NCBI
Target Description M4.2 /REP_DB=WormBase Gene ID /WP=CE17188 /GEN=puf-4 /TR=Q9TYX0 /GB=AAC68995.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067900(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067900 NCBI Caenorhabditis elegans PUF (Pumilio/FBF) domain-containing family member (puf-4) (puf-4) mRNA, complete cds. 11/11 None
M4.2 ENSEMBL cdna:known chromosome:CEL140:IV:3204554:3210578:-1 gene:M4.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000014052 6/11 Cross Hyb Matching Probes None
GENEFINDER00000014055 6/11 Cross Hyb Matching Probes None
GENEFINDER00000014057 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3204559-3210584(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol puf-4
Ensembl M4.2
Entrez Gene 191754 Entrez gene
SwissProt Q8I723 EMBL-EBI
WormBase CE17188 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3723 RNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAN73858 Puf (pumilio/fbf) domain-containing protein 4 [Caenorhabditis elegans] ref|NP_500301.1| PUF (Pumilio/FBF) domain-containing family member (puf-4) [Caenorhabditis elegans] 0.0
blast AAN63424 Hypothetical protein Y73B6BL.38 [Caenorhabditis elegans] ref|NP_741425.1| Y73B6BL.38 [Caenorhabditis elegans] 5.0E-95
blast CAB63369 Hypothetical protein Y45F10A.2 [Caenorhabditis elegans] ref|NP_502606.1| PUF (Pumilio/FBF) domain-containing family member (puf-3) [Caenorhabditis elegans] 2.0E-91
Protein Domains
Database ID Description E-Value
Pfam IPR001313 EMBL-EBI Pumilio/Puf RNA-binding 1.4E-5
Sequence
Target Sequence 
>C. ELEGANS:187883_AT
tatttggctatcgtactggcatcccgtttggcatcggaactccgtggtgtggatctcacc
cacgcatgcattgatcaaaacgggaaccacgtcattcaacagctcctcaagacattgcca
ccgtcttcgtggcagtttctcgtggaattcttctctcgtgacacaaatttcgtcgaagtc
tgcgaggataagtacgtttgccgtgtgattcaatcaacgctggaaacgctttcgaaacgc
gagcagctggtggctcttcgtcgactcgtcggggttattatccggaattgtggacaactg
gcttcgaatgagtttgccaactacgtcgttcgacacgtcatcaaatgtgacgatgccgag
atgaccatctatcgtgacgtcatcattgagaagtgcttgttgcaaaacctgctgtcaatg
tcacaggaaaagtatgcgagtcacatgatccacgtggccggtcagagcacggagcagaag
tggctcgagcgaatccgtgaacgtgtcgtccggaatgcgtcacgtcttgaacgattctcg
tcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATTTGGCTATCGTACTGGCATCCC 610 661 700 Antisense
GGAACTCCGTGGTGTGGATCTCACC 40 531 735 Antisense
ACATTGCCACCGTCTTCGTGGCAGT 566 103 811 Antisense
GGCAGTTTCTCGTGGAATTCTTCTC 214 535 830 Antisense
AAACGCGAGCAGCTGGTGGCTCTTC 125 127 934 Antisense
GCCAACTACGTCGTTCGACACGTCA 519 277 1015 Antisense
GACCATCTATCGTGACGTCATCATT 654 381 1062 Antisense
CCACGTGGCCGGTCAGAGCACGGAG 580 273 1149 Antisense
GCTCGAGCGAATCCGTGAACGTGTC 319 297 1182 Antisense
TGAACGTGTCGTCCGGAATGCGTCA 562 573 1197 Antisense
GTCACGTCTTGAACGATTCTCGTCC 135 465 1218 Antisense
Sequence Source Affymetrix Proprietary Database