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[187875_s_at]

Description C30A5.7B /REP_DB=WormBase Gene ID /WP=CE00099 /GEN=unc-86 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Pou homeodomain
Cluster 6grp_high1_10_10 > 06      4grp_high_8_8 > 25      6grp_total > 06      4grp_total > 39      6grp_cgc > 22      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 345
1 2 3 4 range
A 470.8
(3)
P 		218.4
(-3)
M 		241.8
(-2.4)
P 		241.3
(-2.5)
M 		252
B 530.1
(3)
P 		245.7
(-1.9)
P 		185.2
(-3)
P 		319
(-0.7)
P 		345
C 468.9
(3)
P 		220.5
(-2.3)
P 		185.1
(-3)
P 		249.5
(-1.6)
P 		284
avr 489.9
(3) 		228.2
(-2.5) 		204
(-3) 		269.9
(-1.6) 		286

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 426
1 2 3 4 5 6 range
A 515.4
(3)
P 		356.6
(0.6)
P 		256.2
(-0.9)
P 		190
(-1.9)
P 		172.7
(-2.2)
M 		117.8
(-3)
P 		398
B 486.1
(3)
P 		314.4
(0.1)
P 		203.9
(-1.8)
P 		141.8
(-2.9)
M 		136.2
(-3)
P 		162.9
(-2.5)
P 		350
C 544
(3)
P 		362.1
(0.2)
P 		245.6
(-1.6)
P 		220.3
(-2)
P 		174
(-2.7)
P 		155.4
(-3)
P 		389
avr 515.2
(3) 		344.4
(0.2) 		235.2
(-1.5) 		184
(-2.4) 		161
(-2.7) 		145.4
(-3) 		370
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 136
1 2 3 4 range
A 171.4
(3)
P 		47.4
(-2.5)
M 		63.2
(-1.8)
P 		35.3
(-3)
M 		136
B 169
(3)
P 		60.7
(-2.2)
P 		42.9
(-3)
P 		68
(-1.8)
P 		126
C 155.2
(3)
P 		63.4
(-1.9)
P 		64.5
(-1.8)
P 		42.1
(-3)
P 		113
avr 165.2
(3) 		57.2
(-2.6) 		56.9
(-2.6) 		48.5
(-3) 		117

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 173
1 2 3 4 5 6 range
A 95
(1.1)
P 		126.8
(3)
P 		55.8
(-1.3)
P 		48.2
(-1.8)
P 		52.1
(-1.6)
M 		28.7
(-3)
P 		98
B 169.1
(3)
P 		104.7
(0.1)
P 		49.5
(-2.5)
P 		37.6
(-3)
M 		55.3
(-2.2)
P 		60.7
(-1.9)
P 		132
C 201.6
(3)
P 		110.5
(-0.5)
P 		62.5
(-2.3)
P 		44.3
(-3)
P 		56.3
(-2.5)
P 		52
(-2.7)
P 		157
avr 155.2
(3) 		114
(0.8) 		55.9
(-2.3) 		43.4
(-3) 		54.6
(-2.4) 		47.1
(-2.8) 		112
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187875_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6068
Sequence Type Exemplar sequence
Representative Public ID C30A5.7B NCBI
Target Description C30A5.7B /REP_DB=WormBase Gene ID /WP=CE00099 /GEN=unc-86 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Pou homeodomain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026020(11), NM_066394(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026020 NCBI Caenorhabditis elegans UNCoordinated family member (unc-86) (unc-86) mRNA, complete cds. 11/11 None
NM_066394 NCBI Caenorhabditis elegans UNCoordinated family member (unc-86) (unc-86) mRNA, complete cds. 11/11 None
SNAP00000019638 ENSEMBL cdna:SNAP chromosome:CEL140:III:8213000:8214704:-1 11/11 None
GENEFINDER00000019649 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8213000:8216212:-1 11/11 None
C30A5.7b ENSEMBL cdna:known chromosome:CEL140:III:8212682:8216542:-1 gene:C30A5.7 11/11 None
C30A5.7a ENSEMBL cdna:known chromosome:CEL140:III:8213000:8216542:-1 gene:C30A5.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8212995-8216538(-) 100.0 100.0
Public Domain and Genome References
Ensembl C30A5.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB59176 Uncoordinated protein 86, isoform b [Caenorhabditis elegans] ref|NP_001021191.1| UNCoordinated family member (unc-86) [Caenorhabditis elegans] sp|P13528|UNC86_CAEEL Transcription factor unc-86 (Uncoordinated protein 86) gb|AAA28158.1| unc-86 protein 0.0
blast AAB59175 Uncoordinated protein 86, isoform a [Caenorhabditis elegans] ref|NP_498795.1| UNCoordinated family member (unc-86) [Caenorhabditis elegans] gb|AAA28159.1| unc-86 alternate protein 0.0
blast AAB59176 Uncoordinated protein 86, isoform b [Caenorhabditis elegans] ref|NP_001021191.1| UNCoordinated family member (unc-86) [Caenorhabditis elegans] sp|P13528|UNC86_CAEEL Transcription factor unc-86 (Uncoordinated protein 86) gb|AAA28158.1| unc-86 protein 1.0E-180
Protein Domains
Database ID Description E-Value
scop a.4.1.Homeodomain All alpha proteins; DNA/RNA-binding 3-helical bundle; Homeodomain-like; Homeodomain 2.99999990479657E-22
scop a.4.1.Homeodomain All alpha proteins; DNA/RNA-binding 3-helical bundle; Homeodomain-like; Homeodomain 2.00000006274879E-22
scop a.4.1.Homeodomain All alpha proteins; DNA/RNA-binding 3-helical bundle; Homeodomain-like; Homeodomain 1.00000003137439E-22
scop a.4.1.Homeodomain All alpha proteins; DNA/RNA-binding 3-helical bundle; Homeodomain-like; Homeodomain 7.99999999855967E-23
Pfam IPR001356 EMBL-EBI Homeobox 2.0E-20
Pfam IPR000327 EMBL-EBI POU domain 4.6E-53
Pfam IPR001356 EMBL-EBI Homeobox 2.0E-20
Pfam IPR000327 EMBL-EBI POU domain 4.6E-53
Pfam IPR001356 EMBL-EBI Homeobox 2.0E-20
Pfam IPR000327 EMBL-EBI POU domain 4.6E-53
Pfam IPR001356 EMBL-EBI Homeobox 2.0E-20
Pfam IPR000327 EMBL-EBI POU domain 4.6E-53
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021191.1 1 7-29
NP_498795.1 1 7-29
C30A5.7b 1 7-29
C30A5.7a 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:187875_S_AT
tgccccgccaacttcagatatggacactgatccgagacaattggagacgtttgcagagca
tttcaagcagagaagaataaaattaggagtcacacaggcggacgttggaaaagcacttgc
tcatctgaaaatgcctggtgtcggatcattatcccagtccacgatctgccgcttcgaatc
tctcaccctttcccataacaacatggtcgcactgaaaccaattctacattcctggctgga
gaaagctgaagaggctatgaaacaaaaagatacaattggagatatcaatggaattttgcc
aaatactgataagaaacggaagagaactagtattgctgctcctgagaaaagagaactaga
acagtttttcaaacaacaaccaagaccatctggagaacgaattgcctcaattgccgatcg
attggatttgaagaaaaatgttgttcgcgtctggttttgcaatcaacggcagaaacagaa
aagagatttccgttcccaattccgtgcaagaagtgcagcagcagtaatgggtccacgtgt
gatgccagtttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCCCCGCCAACTTCAGATATGGAC 629 583 789 Antisense
GGAGTCACACAGGCGGACGTTGGAA 499 517 874 Antisense
GTGTCGGATCATTATCCCAGTCCAC 353 487 926 Antisense
CTCACCCTTTCCCATAACAACATGG 255 229 970 Antisense
AACCAATTCTACATTCCTGGCTGGA 446 147 1004 Antisense
GAACTAGTATTGCTGCTCCTGAGAA 615 349 1112 Antisense
GAACGAATTGCCTCAATTGCCGATC 408 347 1183 Antisense
TTGTTCGCGTCTGGTTTTGCAATCA 430 705 1229 Antisense
GTTCCCAATTCCGTGCAAGAAGTGC 15 441 1280 Antisense
TGCAGCAGCAGTAATGGGTCCACGT 343 577 1302 Antisense
TGGGTCCACGTGTGATGCCAGTTTT 542 553 1316 Antisense
Sequence Source Affymetrix Proprietary Database