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[187864_at]

Description M163.4 /REP_DB=WormBase Gene ID /WP=CE12452 /GEN=gfi-3 /TR=Q93903 /GB=CAB01894.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 25      6grp_total > 25      4grp_total > 38      6grp_cgc > 24      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 159
1 2 3 4 range
A 290.3
(3)
P 		210.4
(-0)
M 		131.5
(-3)
A 		275.1
(2.4)
P 		159
B 265.6
(3)
P 		219.9
(0.3)
P 		162.4
(-3)
A 		163.5
(-2.9)
P 		103
C 289.3
(3)
P 		227.2
(-1.3)
P 		201.9
(-3)
P 		250
(0.3)
P 		87
avr 281.7
(3) 		219.2
(-0.2) 		165.3
(-3) 		229.5
(0.3) 		116

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 210
1 2 3 4 5 6 range
A 313.6
(3)
P 		263.1
(1.1)
P 		192.8
(-1.5)
A 		228.4
(-0.2)
A 		154.3
(-3)
P 		232.4
(-0.1)
P 		159
B 303
(3)
P 		244.6
(-0.3)
P 		280.7
(1.7)
A 		215
(-2)
A 		196.8
(-3)
A 		221.9
(-1.6)
P 		106
C 331.9
(3)
P 		281.8
(1.6)
P 		249.2
(0.6)
P 		186.7
(-1.2)
A 		122.2
(-3)
A 		208.9
(-0.5)
P 		210
avr 316.2
(3) 		263.2
(1) 		240.9
(0.1) 		210
(-1) 		157.8
(-3) 		221.1
(-0.6) 		158
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 71
1 2 3 4 range
A 105.7
(3)
P 		45.7
(-2)
M 		34.4
(-3)
A 		40.3
(-2.5)
P 		71
B 84.7
(3)
P 		54.3
(-0.7)
P 		37.6
(-2.7)
A 		34.8
(-3)
P 		50
C 95.8
(3)
P 		65.3
(-0.4)
P 		70.4
(0.2)
P 		42.2
(-3)
P 		54
avr 95.4
(3) 		55.1
(-1.3) 		47.5
(-2.1) 		39.1
(-3) 		56

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 85
1 2 3 4 5 6 range
A 57.8
(-1.2)
P 		93.6
(3)
P 		42
(-3)
A 		57.9
(-1.2)
A 		46.6
(-2.5)
P 		56.6
(-1.3)
P 		52
B 105.4
(3)
P 		81.4
(0)
P 		68.2
(-1.6)
A 		56.9
(-3)
A 		79.9
(-0.2)
A 		82.7
(0.2)
P 		49
C 123
(3)
P 		86
(0.4)
P 		63.4
(-1.2)
P 		37.6
(-3)
A 		39.5
(-2.9)
A 		69.9
(-0.7)
P 		85
avr 95.4
(3) 		87
(1.9) 		57.9
(-2) 		50.8
(-3) 		55.3
(-2.4) 		69.7
(-0.5) 		45
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187864_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17358
Sequence Type Exemplar sequence
Representative Public ID M163.4 NCBI
Target Description M163.4 /REP_DB=WormBase Gene ID /WP=CE12452 /GEN=gfi-3 /TR=Q93903 /GB=CAB01894.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078008(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078008 NCBI Caenorhabditis elegans GEI-4(Four) Interacting protein family member (gfi-3) (gfi-3) mRNA, complete cds. 11/11 None
SNAP00000011159 ENSEMBL cdna:SNAP chromosome:CEL140:X:14480493:14484391:1 11/11 None
GENEFINDER00000011165 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:14480493:14484391:1 11/11 None
M163.4 ENSEMBL cdna:known chromosome:CEL140:X:14480491:14484575:1 gene:M163.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:14480492-14484391(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol gfi-3
Ensembl M163.4
Entrez Gene 181544 Entrez gene
SwissProt Q93903 EMBL-EBI
WormBase CE12452 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01894 Hypothetical protein M163.4 [Caenorhabditis elegans] ref|NP_510409.1| GEI-4(Four) Interacting protein family member (gfi-3) [Caenorhabditis elegans] 0.0
blast CAE63169 Hypothetical protein CBG07492 [Caenorhabditis briggsae] 0.0
blast CAC70137 Hypothetical protein Y66D12A.17 [Caenorhabditis elegans] ref|NP_499485.1| Y66D12A.17 [Caenorhabditis elegans] 1.0E-143
Sequence
Target Sequence 
>C. ELEGANS:187864_AT
ttgaatctcgcagcacactctgaactagaatgcaagcttcgacaagctcttctcctcgcc
gcagccggaaaagaacgggcaagtgttgaagtgttggagggattgaacgttgtggacatt
cgtggaagaattcgaattcacatgcaaacaggatgcatttacacggctagtcaagaattt
agaaaagctgcaagagaatttgcagacgccttgagtcttgctgagaacaccacactgaag
gacattgtgccaatggtcaagagaagacaagcaactatgctgatgtgccatggtcagtat
gtcgaatgtctgaaacttttgaaagaatgcaatgaaggcattgaacagtttggatcattt
accgagaaggcgtgctactacatgacagcggccagatgtcatcgattgctagggaaagat
ccgcggatgtggttgaaaaaatcccgtgccctggtccgcaacgggcaatggccggcgttt
accaagctcgtcctctccgaaattgctgctcttcatgacgtcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGAATCTCGCAGCACACTCTGAAC 653 703 1792 Antisense
TGCAAGCTTCGACAAGCTCTTCTCC 289 577 1822 Antisense
TCCTCGCCGCAGCCGGAAAAGAACG 481 591 1844 Antisense
GGATGCATTTACACGGCTAGTCAAG 274 511 1942 Antisense
TTGCAGACGCCTTGAGTCTTGCTGA 430 703 1991 Antisense
GTCTTGCTGAGAACACCACACTGAA 126 469 2006 Antisense
GCAACTATGCTGATGTGCCATGGTC 527 319 2062 Antisense
GGCGTGCTACTACATGACAGCGGCC 240 541 2160 Antisense
GACAGCGGCCAGATGTCATCGATTG 681 367 2175 Antisense
GTGGTTGAAAAAATCCCGTGCCCTG 243 479 2220 Antisense
AAATTGCTGCTCTTCATGACGTCAA 361 115 2291 Antisense
Sequence Source Affymetrix Proprietary Database