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[187858_at]

Description Y23H5A.5 /REP_DB=WormBase Gene ID /WP=CE18361 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:O76614 protein_id:AAC64631.1
Cluster 6grp_high1_10_10 > 05      4grp_high_8_8 > 43      6grp_total > 14      4grp_total > 52      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 183
1 2 3 4 range
A 219.8
(-0.3)
P 		250.6
(1.8)
P 		268.8
(3)
P 		180
(-3)
P 		89
B 345.7
(3)
P 		218.9
(-1.2)
P 		214.2
(-1.3)
P 		162.6
(-3)
P 		183
C 208
(-1.1)
P 		303.3
(3)
P 		191
(-1.9)
P 		164.8
(-3)
A 		139
avr 257.8
(3) 		257.6
(3) 		224.7
(0.8) 		169.1
(-3) 		89

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 259
1 2 3 4 5 6 range
A 348.4
(3)
P 		271.9
(1)
P 		261.6
(0.7)
P 		190.1
(-1.2)
P 		185.8
(-1.3)
P 		119.7
(-3)
P 		229
B 328.5
(3)
P 		287.9
(1.8)
P 		143.2
(-2.6)
P 		183.4
(-1.4)
P 		157.6
(-2.2)
P 		130.5
(-3)
P 		198
C 356.3
(3)
P 		268.5
(1)
P 		266.8
(0.9)
P 		201.1
(-0.6)
P 		150.1
(-1.8)
P 		97.1
(-3)
A 		259
avr 344.4
(3) 		276.1
(1.2) 		223.9
(-0.2) 		191.5
(-1) 		164.5
(-1.7) 		115.8
(-3) 		229
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 80
(3)
P 		54.4
(0.1)
P 		70.3
(1.9)
P 		26.3
(-3)
P 		54
B 110.2
(3)
P 		54.1
(-1.5)
P 		49.6
(-1.8)
P 		34.6
(-3)
P 		76
C 68.9
(1.2)
P 		87.2
(3)
P 		66.6
(0.9)
P 		27.8
(-3)
A 		59
avr 86.4
(3) 		65.2
(0.8) 		62.2
(0.4) 		29.6
(-3) 		57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 103
1 2 3 4 5 6 range
A 64.2
(0.1)
P 		96.7
(3)
P 		57
(-0.5)
P 		48.2
(-1.3)
P 		56.1
(-0.6)
P 		29.1
(-3)
P 		68
B 114.3
(3)
P 		95.8
(1.6)
P 		34.8
(-3)
P 		48.6
(-2)
P 		64
(-0.8)
P 		48.7
(-2)
P 		80
C 132.1
(3)
P 		81.9
(-0)
P 		67.9
(-0.9)
P 		40.4
(-2.5)
P 		48.6
(-2)
P 		32.5
(-3)
A 		100
avr 103.5
(3) 		91.5
(1.9) 		53.2
(-1.5) 		45.7
(-2.2) 		56.2
(-1.3) 		36.8
(-3) 		67
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187858_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.123
Sequence Type Exemplar sequence
Representative Public ID Y23H5A.5 NCBI
Target Description Y23H5A.5 /REP_DB=WormBase Gene ID /WP=CE18361 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:O76614 protein_id:AAC64631.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170829(11), NM_058607(11), NM_058608(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_170829 NCBI Caenorhabditis elegans alpha-CaTuliN (catenin/vinculin related) family member (ctn-1) (ctn-1) mRNA, complete cds. 11/11 None
NM_058607 NCBI Caenorhabditis elegans alpha-CaTuliN (catenin/vinculin related) family member (ctn-1) (ctn-1) mRNA, complete cds. 11/11 None
NM_058608 NCBI Caenorhabditis elegans alpha-CaTuliN (catenin/vinculin related) family member (ctn-1) (ctn-1) mRNA, complete cds. 10/11 None
GENEFINDER00000016946 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2613263:2615442:1 10/11 None
SNAP00000016934 ENSEMBL cdna:SNAP chromosome:CEL140:I:2613326:2615938:1 10/11 None
Y23H5A.5d.2 ENSEMBL cdna:known chromosome:CEL140:I:2606755:2617361:1 gene:Y23H5A.5 11/11 None
Y23H5A.5d.1 ENSEMBL cdna:known chromosome:CEL140:I:2606755:2617337:1 gene:Y23H5A.5 11/11 None
Y23H5A.5b ENSEMBL cdna:known chromosome:CEL140:I:2606835:2616056:1 gene:Y23H5A.5 10/11 None
Y23H5A.5a ENSEMBL cdna:known chromosome:CEL140:I:2606835:2617276:1 gene:Y23H5A.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
171818 4 3 NM_058607 187858_at (11)
NM_058608 187858_at (10)
NM_170829 187858_at (11)
NM_170830 185467_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2614031-2617276(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y23H5A.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_740813 alpha-CaTuliN (catenin/vinculin related) family member (ctn-1) [Caenorhabditis elegans] gb|AAM54172.1| Alpha-catulin (catenin/vinculin related) protein 1, isoform d [Caenorhabditis elegans] 0.0
blast NP_491009 alpha-CaTuliN (catenin/vinculin related) family member (ctn-1) [Caenorhabditis elegans] gb|AAL77190.1| Alpha-catulin (catenin/vinculin related) protein 1, isoform b [Caenorhabditis elegans] 0.0
blast NP_491008 alpha-CaTuliN (catenin/vinculin related) family member (ctn-1) [Caenorhabditis elegans] gb|AAL77189.1| Alpha-catulin (catenin/vinculin related) protein 1, isoform a [Caenorhabditis elegans] 0.0
blast CAE68998 Hypothetical protein CBG14987 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006077 EMBL-EBI Vinculin/alpha-catenin 8.4E-40
Pfam IPR006077 EMBL-EBI Vinculin/alpha-catenin 1.1E-13
Pfam IPR006077 EMBL-EBI Vinculin/alpha-catenin 1.1E-13
Pfam IPR006077 EMBL-EBI Vinculin/alpha-catenin 1.1E-13
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491008.2 1 769-791
Y23H5A.5a 1 769-791
Sequence
Target Sequence 
>C. ELEGANS:187858_AT
tgaagacgacacacgacctcttcacccaggcggagttctttgccgagcaggcgaatcaga
tgtatcggacagttcgagagttctcatatgaagttccgggctctgcggaaaaatccgatt
tgtcagcgattcttgagaggatcccacttcactgccaacagctacaggttatggttaaga
gtccgaccgtcggaaaaactgcgactttcgggaaagtggattcggtgattcaggagacca
agaatttgatgaacgaaatcgcgaagctggtcactgcgtcgtttgtgtgtgccacgaagt
atgaaatcgagtttcgcggaggttctgtcaatgggcggacgggtgccgacggtttggcgc
cggacaccaagcatccgacgagccgcgccgccgacctcttcgcacctttccgccaacaac
tcatcaagcatacacatttagtagtacaattgcgagctgacgtcatcaccgttccgcggc
cgcccacaggatctctcccattttgtttttcagagcccta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAAGACGACACACGACCTCTTCAC 135 575 173 Antisense
GAGTTCTTTGCCGAGCAGGCGAATC 61 397 205 Antisense
CTCATATGAAGTTCCGGGCTCTGCG 497 227 255 Antisense
GATTCTTGAGAGGATCCCACTTCAC 217 429 300 Antisense
CACTTCACTGCCAACAGCTACAGGT 422 197 317 Antisense
TATGGTTAAGAGTCCGACCGTCGGA 129 41 342 Antisense
AAATCGCGAAGCTGGTCACTGCGTC 118 117 428 Antisense
GCGTCGTTTGTGTGTGCCACGAAGT 487 289 448 Antisense
AGTTTCGCGGAGGTTCTGTCAATGG 509 61 482 Antisense
TGTCAATGGGCGGACGGGTGCCGAC 526 557 498 Antisense
TCCCATTTTGTTTTTCAGAGCCCTA 693 593 668 Antisense
Sequence Source Affymetrix Proprietary Database