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[187857_at]

Description F35H12.2 /REP_DB=WormBase Gene ID /WP=CE04511 /CHR=X /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:Q20078 protein_id:AAA83178.1
Cluster 6grp_high1_10_10 > 02      4grp_high_8_8 > 34      6grp_total > 24      4grp_total > 46      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 96
1 2 3 4 range
A 116.6
(3)
P 		61.6
(-1.5)
P 		43.8
(-3)
A 		62.8
(-1.4)
P 		73
B 135.3
(3)
P 		112.4
(0.8)
P 		73
(-3)
P 		76.3
(-2.7)
P 		62
C 139.9
(3)
P 		100.8
(-0)
P 		61.7
(-3)
P 		78.1
(-1.7)
P 		78
avr 130.6
(3) 		91.6
(-0.3) 		59.5
(-3) 		72.4
(-1.9) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 71
1 2 3 4 5 6 range
A 89.2
(1.5)
P 		90.1
(1.6)
P 		104.9
(3)
P 		63.8
(-1)
P 		66.2
(-0.8)
P 		43
(-3)
A 		62
B 109.1
(3)
P 		72.1
(-2.3)
P 		81.8
(-0.9)
P 		69.3
(-2.7)
P 		67.3
(-3)
P 		67.1
(-3)
P 		42
C 94.8
(2.2)
P 		103.2
(3)
P 		68.4
(-0.2)
P 		62.8
(-0.7)
A 		37.8
(-3)
P 		75.1
(0.4)
P 		65
avr 97.7
(3) 		88.5
(1.6) 		85
(1.1) 		65.3
(-1.8) 		57.1
(-3) 		61.7
(-2.3) 		41
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 37
1 2 3 4 range
A 42.4
(3)
P 		13.4
(-2.2)
P 		11.5
(-2.6)
A 		9.2
(-3)
P 		33
B 43.2
(3)
P 		27.8
(-0.4)
P 		16.9
(-2.9)
P 		16.3
(-3)
P 		27
C 46.3
(3)
P 		29
(-0.1)
P 		21.5
(-1.5)
P 		13.2
(-3)
P 		33
avr 44
(3) 		23.4
(-1) 		16.6
(-2.3) 		12.9
(-3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 16.4
(-1.4)
P 		32.1
(3)
P 		22.8
(0.4)
P 		16.2
(-1.4)
P 		20
(-0.4)
P 		10.5
(-3)
A 		22
B 38
(3)
P 		24
(-1.3)
P 		19.9
(-2.5)
P 		18.4
(-3)
P 		27.3
(-0.3)
P 		25
(-1)
P 		20
C 35.1
(3)
P 		31.5
(2.1)
P 		17.4
(-1.6)
P 		12.6
(-2.9)
A 		12.2
(-3)
P 		25.1
(0.4)
P 		23
avr 29.8
(3) 		29.2
(2.7) 		20
(-1.2) 		15.7
(-3) 		19.8
(-1.3) 		20.2
(-1.1) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187857_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18574
Sequence Type Exemplar sequence
Representative Public ID F35H12.2 NCBI
Target Description F35H12.2 /REP_DB=WormBase Gene ID /WP=CE04511 /CHR=X /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:Q20078 protein_id:AAA83178.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171620(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171620 NCBI Caenorhabditis elegans F35H12.7 (F35H12.7) mRNA, complete cds. 9/11 None
F35H12.7 ENSEMBL cdna:known chromosome:CEL140:X:933801:934903:1 gene:F35H12.7 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000028612 7/11 Cross Hyb Matching Probes None
GENEFINDER00000028613 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:925008-934903(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35H12.7
Entrez Gene 266943 Entrez gene
SwissProt Q8MQ58 EMBL-EBI
WormBase CE30974 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM54185 Hypothetical protein F35H12.7 [Caenorhabditis elegans] ref|NP_741712.1| F35H12.7 [Caenorhabditis elegans] 4.0E-79
blast G89453 protein F35H12.2 [imported] - Caenorhabditis elegans 4.0E-79
blast CAE63598 Hypothetical protein CBG08089 [Caenorhabditis briggsae] 5.0E-19
Sequence
Target Sequence 
>C. ELEGANS:187857_AT
agtgacgccacatttttcttcaacttttcaactgcagatggatcatacggaaaccacaac
ttcaaataatatcggatacaattcgtcgtctgaatcattcgcggttcgttttatgcaaac
gccgtcagctgttttgaaaataaccaatggagagatgactgaggacaataacaatatgct
gcatggaattaatggtgttgacttgctggatttgcaaagtactgataacgacgatcaata
ctcaaattcaagcagtttggagtcaaggaactctctgacaaatcatcagggtaaagctga
agattcaacgatggtgacggtgaatctggatcaacttcctgctcggaggactcttttgaa
atgtctagtacttgtagtgttcgggaagagttatacgaaggtaattttccagatgctcag
attttcaggcttcctaatgaggcgaggagtttatccgggtac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTGACGCCACATTTTTCTTCAACT 45 61 2925 Antisense
TTTTTCTTCAACTTTTCAACTGCAG 394 675 2937 Antisense
ATATCGGATACAATTCGTCGTCTGA 247 21 2993 Antisense
TCGTCGTCTGAATCATTCGCGGTTC 464 605 3007 Antisense
ATCATTCGCGGTTCGTTTTATGCAA 344 31 3018 Antisense
ATGCAAACGCCGTCAGCTGTTTTGA 636 43 3037 Antisense
AATGGTGTTGACTTGCTGGATTTGC 412 163 3115 Antisense
TGAATCTGGATCAACTTCCTGCTCG 691 575 3245 Antisense
TTCCTGCTCGGAGGACTCTTTTGAA 312 697 3260 Antisense
AGATGCTCAGATTTTCAGGCTTCCT 535 67 3335 Antisense
TGAGGCGAGGAGTTTATCCGGGTAC 65 559 3362 Antisense
Sequence Source Affymetrix Proprietary Database