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[187856_s_at] acetylcholinesterase

Description Y48B6A.15 /REP_DB=WormBase Gene ID /WP=CE22131 /CHR=2 /FEA=Sanger Annotation /DEF=(HINXTON) TR:Q9U2A5 protein_id:CAB54443.1
Cluster 6grp_total > 92      4grp_total > 02      6grp_cgc > 90      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 71
1 2 3 4 range
A 5.8
(-3)
A 		13.5
(-2.3)
A 		28.5
(-1)
A 		74.9
(3)
A 		69
B 4.5
(-3)
A 		7
(-2)
A 		9
(-1.1)
A 		19
(3)
A 		15
C 32.6
(0.1)
A 		17.6
(-1.5)
A 		3.9
(-3)
A 		60.1
(3)
A 		56
avr 14.3
(-2.8) 		12.7
(-3) 		13.8
(-2.8) 		51.3
(3) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 42.1
(3)
A 		17.9
(-1.1)
A 		33.6
(1.6)
A 		6.5
(-3)
A 		17.2
(-1.2)
A 		9.2
(-2.5)
A 		36
B 23.9
(0.1)
A 		6.9
(-2.3)
A 		44.8
(3)
A 		6.4
(-2.3)
A 		29.2
(0.8)
A 		1.7
(-3)
A 		43
C 4.6
(-3)
A 		5.7
(-2.9)
A 		8.6
(-2.5)
A 		54.3
(3)
A 		42.4
(1.6)
A 		16
(-1.6)
A 		50
avr 23.5
(1.2) 		10.2
(-2.7) 		29
(2.8) 		22.4
(0.9) 		29.6
(3) 		9
(-3) 		21
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 2.1
(-3)
A 		2.9
(-2.5)
A 		7.5
(0.6)
A 		11
(3)
A 		9
B 1.4
(-3)
A 		1.7
(-2.3)
A 		2.1
(-1.4)
A 		4.1
(3)
A 		3
C 10.8
(3)
A 		5.1
(-0.6)
A 		1.4
(-3)
A 		10.1
(2.6)
A 		9
avr 4.8
(-1.2) 		3.2
(-3) 		3.7
(-2.4) 		8.4
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 7.8
(3)
A 		6.4
(1.6)
A 		7.3
(2.5)
A 		1.6
(-3)
A 		5.2
(0.5)
A 		2.2
(-2.4)
A 		6
B 8.3
(1.1)
A 		2.3
(-2.1)
A 		10.9
(2.5)
A 		1.7
(-2.4)
A 		11.9
(3)
A 		0.6
(-3)
A 		11
C 1.7
(-3)
A 		1.7
(-3)
A 		2.2
(-2.8)
A 		10.9
(1.6)
A 		13.7
(3)
A 		5.4
(-1.2)
A 		12
avr 5.9
(-0.5) 		3.5
(-2.4) 		6.8
(0.2) 		4.7
(-1.4) 		10.3
(3) 		2.7
(-3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187856_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3514
Sequence Type Exemplar sequence
Representative Public ID Y48B6A.15 NCBI
Target Description Y48B6A.15 /REP_DB=WormBase Gene ID /WP=CE22131 /CHR=2 /FEA=Sanger Annotation /DEF=(HINXTON) TR:Q9U2A5 protein_id:CAB54443.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064561(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064561 NCBI Caenorhabditis elegans Y48B6A.7 (acetylcholinesterase) mRNA, complete cds. 11/11 A 
SNAP00000022963 ENSEMBL cdna:SNAP chromosome:CEL140:II:14198206:14205085:1 11/11 None
GENEFINDER00000022983 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14198339:14200542:1 11/11 None
Y48B6A.7 ENSEMBL cdna:known chromosome:CEL140:II:14197937:14205344:1 gene:Y48B6A.7 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14198335-14200546(+) 94.21 100.0
Public Domain and Genome References
Gene Title acetylcholinesterase
Gene Symbol ace-4
Ensembl Y48B6A.7
Entrez Gene 175074 Entrez gene
SwissProt O61372 EMBL-EBI
WormBase CE26734 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
4104 cholinesterase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB54444 Hypothetical protein Y48B6A.7 [Caenorhabditis elegans] ref|NP_496962.1| Y48B6A.7 [Caenorhabditis elegans] gb|AAC14017.1| acetylcholinesterase [Caenorhabditis elegans] 0.0
blast AAF80378 acetylcholinesterase [Caenorhabditis briggsae] sp|Q9NDG8|ACE4_CAEBR Acetylcholinesterase 4 precursor (AChE 4) 0.0
blast CAE59451 Hypothetical protein CBG02827 [Caenorhabditis briggsae] 0.0
blast T26999 hypothetical protein Y48B6A.15 - Caenorhabditis elegans 1.0E-113
Protein Domains
Database ID Description E-Value
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 1.0E-126
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 1.0E-126
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 2.7E-33
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 6.6E-84
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496962.1 1 7-29
Y48B6A.7 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:187856_S_AT
tggagaggactctacgatgcgacaaaaccggcgaacgcctgttttcagacaagggacaat
tataacacgagcttctggggaagtgaaatgtggaacgcgaatactcaaattagtgaggac
tgtttatacctgaatatttgggctcccgcggatgcttacaatctcacagtgatggtgtgg
ttttttggagggggcttctactctggaagcccgtcattgagtatctacgacgggaaagcc
ttaacctccacccaaaacgtgattgtcgtgaacatcaactaccgtctcggcccgttcgga
tttctctacctgggccacccagatgctcccggaaacatgggcctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGAGAGGACTCTACGATGCGACAA 587 549 271 Antisense
GATGCGACAAAACCGGCGAACGCCT 279 407 286 Antisense
GGCGAACGCCTGTTTTCAGACAAGG 450 499 300 Antisense
GGGACAATTATAACACGAGCTTCTG 321 495 323 Antisense
GGATGCTTACAATCTCACAGTGATG 616 513 420 Antisense
TTTGGAGGGGGCTTCTACTCTGGAA 701 661 454 Antisense
TGGAAGCCCGTCATTGAGTATCTAC 540 545 474 Antisense
GAGTATCTACGACGGGAAAGCCTTA 553 395 489 Antisense
TAACCTCCACCCAAAACGTGATTGT 207 637 512 Antisense
TGTCGTGAACATCAACTACCGTCTC 462 557 534 Antisense
AGATGCTCCCGGAAACATGGGCCTC 183 67 591 Antisense
Sequence Source Affymetrix Proprietary Database