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[187851_at]

Description C25E10.3 /REP_DB=WormBase Gene ID /WP=CE06865 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:Q18154 protein_id:AAA92309.1
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 05      4grp_highest_8_8 > 57      4grp_high_8_8 > 34      6grp_highest_nonorm > 70      6grp_total > 06      4grp_total > 45      6grp_highest_sub > De      6grp_cgc > 21      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 454
1 2 3 4 range
A 612.2
(3)
P 		394.2
(-0.1)
P 		185.7
(-3)
P 		223.1
(-2.5)
P 		427
B 588.1
(3)
P 		385.9
(0.2)
P 		216.7
(-2.2)
P 		158.2
(-3)
P 		430
C 600
(3)
P 		331.2
(-1.2)
P 		296.6
(-1.7)
P 		211.9
(-3)
P 		388
avr 600.1
(3) 		370.4
(-0.4) 		233
(-2.5) 		197.7
(-3) 		402

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 483
1 2 3 4 5 6 range
A 430.9
(3)
P 		356.3
(1)
P 		302.3
(-0.4)
P 		253.9
(-1.6)
P 		232.7
(-2.2)
P 		202.3
(-3)
P 		229
B 443.4
(3)
P 		357
(1.2)
P 		290.7
(-0.2)
P 		164.1
(-2.8)
P 		195.8
(-2.1)
P 		154.1
(-3)
P 		289
C 568.4
(3)
P 		364.2
(0.5)
P 		315.1
(-0.1)
P 		85.5
(-3)
P 		239.2
(-1.1)
P 		186.4
(-1.7)
P 		483
avr 480.9
(3) 		359.2
(0.7) 		302.7
(-0.4) 		167.8
(-3) 		222.6
(-1.9) 		180.9
(-2.7) 		313
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 190
1 2 3 4 range
A 222.9
(3)
P 		85.6
(-1.3)
P 		48.5
(-2.5)
P 		32.7
(-3)
P 		190
B 187.6
(3)
P 		95.3
(-0.6)
P 		50.2
(-2.4)
P 		33.7
(-3)
P 		154
C 198.6
(3)
P 		95.2
(-0.8)
P 		103.4
(-0.5)
P 		35.7
(-3)
P 		163
avr 203
(3) 		92
(-0.9) 		67.4
(-1.8) 		34
(-3) 		169

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 194
1 2 3 4 5 6 range
A 79.4
(-0.7)
P 		126.7
(3)
P 		65.8
(-1.7)
P 		64.4
(-1.8)
P 		70.2
(-1.4)
P 		49.3
(-3)
P 		77
B 154.3
(3)
P 		118.8
(1.1)
P 		70.6
(-1.5)
P 		43.5
(-3)
P 		79.5
(-1.1)
P 		57.5
(-2.2)
P 		111
C 210.7
(3)
P 		111.1
(-0.1)
P 		80.2
(-1)
P 		17.2
(-3)
P 		77.4
(-1.1)
P 		62.4
(-1.6)
P 		194
avr 148.1
(3) 		118.9
(1.4) 		72.2
(-1.3) 		41.7
(-3) 		75.7
(-1.1) 		56.4
(-2.2) 		106
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187851_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11393
Sequence Type Exemplar sequence
Representative Public ID C25E10.3 NCBI
Target Description C25E10.3 /REP_DB=WormBase Gene ID /WP=CE06865 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:Q18154 protein_id:AAA92309.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072939(11), NM_072940(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072939 NCBI Caenorhabditis elegans Serpentine Receptor, class SX family member (srsx-34) (srsx-34) mRNA, complete cds. 11/11 A 
NM_072940 NCBI Caenorhabditis elegans Serpentine Receptor, class SX family member (srsx-34) (srsx-34) mRNA, complete cds. 11/11 None
SNAP00000038734 ENSEMBL cdna:SNAP chromosome:CEL140:V:9039080:9041003:1 11/11 None
GENEFINDER00000038745 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9039080:9046234:1 11/11 None
C25E10.3a ENSEMBL cdna:known chromosome:CEL140:V:9039080:9041361:1 gene:C25E10.3 11/11 None
C25E10.3b ENSEMBL cdna:known chromosome:CEL140:V:9039080:9042876:1 gene:C25E10.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
179281 3 2 NM_001028507 175428_at (11)
NM_072939 175428_at (11), 187851_at (11)
NM_072940 187851_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9039080-9041004(+) 100.0 100.0
Public Domain and Genome References
Ensembl C25E10.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68203 Serpentine receptor, class sx protein 34, isoform b [Caenorhabditis elegans] ref|NP_505340.1| Serpentine Receptor, class SX family member (srsx-34) [Caenorhabditis elegans] 0.0
blast AAA92309 Serpentine receptor, class sx protein 34, isoform a [Caenorhabditis elegans] ref|NP_505341.1| Serpentine Receptor, class SX family member (srsx-34) [Caenorhabditis elegans] 0.0
blast AAA92310 Cytochrome p450 family protein 33B1 [Caenorhabditis elegans] ref|NP_505342.2| CYtochrome P450 family member (cyp-33B1) [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-118
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 3.7E-60
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505340.1 14 33-55,70-92,105-122,132-154,166-188,208-230,280-302,312-331,343-361,390-412,432-451,461-483,495-517,527-549
NP_505341.1 12 33-55,70-92,105-122,132-154,166-188,208-230,280-302,312-331,343-361,390-412,432-451,461-483
C25E10.3a 12 33-55,70-92,105-122,132-154,166-188,208-230,280-302,312-331,343-361,390-412,432-451,461-483
C25E10.3b 14 33-55,70-92,105-122,132-154,166-188,208-230,280-302,312-331,343-361,390-412,432-451,461-483,495-517,527-549
SNAP00000038734 12 33-55,70-92,105-122,132-154,166-185,189-211,262-284,294-313,325-343,372-394,414-433,443-465
GENEFINDER00000038745 15 33-55,70-92,105-122,132-154,166-188,208-230,280-302,312-331,343-361,395-417,455-477,487-509,530-552,572-594,601-623
Sequence
Target Sequence 
>C. ELEGANS:187851_AT
atgtcatctaccatactacttctctatattttctgtcatttcggaaaaattctcccacag
cacaaagtcttcttattcttcacaattgcatttggcgcagtctatataatcacgtatcca
tttgagtttacaagtaatccagttgccaaatacaatgaaaccactcgcgctgggtgcaac
cctgaggagtactcatggtgttcgactgctttggcggtcaatccaatgctatatctttcc
gtaactatattggttgccggaatgtccattccgataatgagcactgctttggatacagtt
tactcaagaattctgggaaatattgaccaatctgttgctcatggtgcaatggcagtaatc
gatgacatcttgtacatgatcacaccaatttttacaacaacaatgtttacattatacgga
attggcccattatggattgtcaaatcgatggcttttggtataattgcgcta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTCATCTACCATACTACTTCTCT 232 49 973 Antisense
AAATTCTCCCACAGCACAAAGTCTT 102 115 1019 Antisense
AGTCTTCTTATTCTTCACAATTGCA 700 61 1038 Antisense
TACAATGAAACCACTCGCGCTGGGT 54 637 1123 Antisense
TGGGTGCAACCCTGAGGAGTACTCA 236 553 1143 Antisense
GGAGTACTCATGGTGTTCGACTGCT 561 517 1158 Antisense
TGTTCGACTGCTTTGGCGGTCAATC 42 563 1171 Antisense
ATCCAATGCTATATCTTTCCGTAAC 39 41 1193 Antisense
GACCAATCTGTTGCTCATGGTGCAA 85 385 1297 Antisense
GACATCTTGTACATGATCACACCAA 580 369 1336 Antisense
GATGGCTTTTGGTATAATTGCGCTA 160 407 1419 Antisense
Sequence Source Affymetrix Proprietary Database