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[187837_at]

Description F26D12.A /REP_DB=WormBase Gene ID /WP=CE20726 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:Q19808 protein_id:AAA93441.1
Cluster 6grp_total > 67      4grp_total > 03      6grp_cgc > 48      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 935
1 2 3 4 range
A 231
(-1.4)
P 		57.1
(-2.7)
A 		11
(-3)
A 		835.9
(3)
P 		825
B 189.4
(-1.9)
P 		102.9
(-2.5)
A 		28.1
(-3)
A 		945.8
(3)
P 		918
C 170.5
(-2)
P 		110.2
(-2.5)
A 		41.8
(-3)
A 		791.6
(3)
P 		750
avr 197
(-1.8) 		90.1
(-2.5) 		27
(-3) 		857.8
(3) 		831

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 739
1 2 3 4 5 6 range
A 83.3
(-2.4)
A 		85
(-2.4)
A 		82.8
(-2.4)
A 		8.3
(-3)
A 		100.8
(-2.2)
A 		746.8
(3)
P 		739
B 75.8
(-2.4)
A 		35.9
(-2.8)
A 		11.6
(-3)
A 		108.3
(-2.1)
P 		86.5
(-2.3)
M 		678.8
(3)
P 		667
C 59.7
(-2.6)
A 		55.4
(-2.6)
A 		55.5
(-2.6)
A 		10
(-3)
A 		97.7
(-2.2)
M 		683.1
(3)
P 		673
avr 72.9
(-2.7) 		58.8
(-2.8) 		50
(-2.9) 		42.2
(-3) 		95
(-2.5) 		702.9
(3) 		661
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 199
1 2 3 4 range
A 84.1
(1.1)
P 		12.4
(-2.5)
A 		2.9
(-3)
A 		122.4
(3)
P 		120
B 60.4
(-1.3)
P 		25.4
(-2.4)
A 		6.5
(-3)
A 		201.6
(3)
P 		195
C 56.4
(-0.9)
P 		31.7
(-2.1)
A 		14.6
(-3)
A 		133.5
(3)
P 		119
avr 67
(-0.6) 		23.2
(-2.4) 		8
(-3) 		152.5
(3) 		145

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 251
1 2 3 4 5 6 range
A 15.4
(-2.6)
A 		30.2
(-2.1)
A 		18
(-2.5)
A 		2.1
(-3)
A 		30.4
(-2.1)
A 		181.8
(3)
P 		180
B 26.4
(-2.4)
A 		11.9
(-2.8)
A 		2.8
(-3)
A 		28.7
(-2.4)
P 		35.1
(-2.2)
M 		253.1
(3)
P 		250
C 22.1
(-2.5)
A 		16.9
(-2.6)
A 		14.1
(-2.7)
A 		2
(-3)
A 		31.6
(-2.2)
M 		228.7
(3)
P 		227
avr 21.3
(-2.7) 		19.7
(-2.7) 		11.6
(-3) 		10.9
(-3) 		32.4
(-2.4) 		221.2
(3) 		210
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187837_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19183
Sequence Type Exemplar sequence
Representative Public ID F26D12.A NCBI
Target Description F26D12.A /REP_DB=WormBase Gene ID /WP=CE20726 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:Q19808 protein_id:AAA93441.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027976(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027976 NCBI Caenorhabditis elegans ForKHead transcription factor family member (fkh-7) (fkh-7) mRNA, complete cds. 11/11 None
SNAP00000039369 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5584670:5586279:1 11/11 None
GENEFINDER00000039374 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5584670:5585345:1 11/11 None
F26D12.1a ENSEMBL cdna:known chromosome:CEL140:IV:5584670:5610349:1 gene:F26D12.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177340 4 1 NM_001027976 174839_at (11), 187837_at (11)
NM_001027979 174839_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5584675-5585351(+) 100.0 100.0
Public Domain and Genome References
Ensembl F26D12.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAR85907 Forkhead transcription factor family protein 7, isoform a [Caenorhabditis elegans] ref|NP_001023147.1| ForKHead transcription factor family member (fkh-7) [Caenorhabditis elegans] 0.0
blast AAR85908 Forkhead transcription factor family protein 7, isoform b [Caenorhabditis elegans] ref|NP_001023148.1| ForKHead transcription factor family member (fkh-7) [Caenorhabditis elegans] 0.0
blast CAE72690 Hypothetical protein CBG19911 [Caenorhabditis briggsae] 0.0
blast T29516 hypothetical protein F26D12.a - Caenorhabditis elegans 9.0E-79
blast AAR85907 Forkhead transcription factor family protein 7, isoform a [Caenorhabditis elegans] ref|NP_001023147.1| ForKHead transcription factor family member (fkh-7) [Caenorhabditis elegans] 2.0E-64
blast AAR85907 Forkhead transcription factor family protein 7, isoform a [Caenorhabditis elegans] ref|NP_001023147.1| ForKHead transcription factor family member (fkh-7) [Caenorhabditis elegans] 5.0E-64
blast T29516 hypothetical protein F26D12.a - Caenorhabditis elegans 5.0E-64
Protein Domains
Database ID Description E-Value
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 4.00000001268431E-29
Pfam IPR001766 EMBL-EBI Fork head transcription factor 1.8E-29
Sequence
Target Sequence 
>C. ELEGANS:187837_AT
gctgacaccagcacaacatttatcctcaacatctacagttacatctccaactagtcaaaa
taatcatttattggcagaagacgaagagcaaatagacgttgttcatgaagaagaggaaga
agatatggaagaaatgaaaatggaaattgaaatgcaatcaaagagtttacatcagaaacc
atcaggattacgaagaagttttcgacctttgattgcttcggaactaagatctggaggaat
tcataaaactagtagtgactcgaatttaaagcgattggcaggaccatcatcatctgattc
tgctggaacttctggttatgggtaccttccccgtccagcatcaaatcttccagctggaat
aaatcatgaattcaaaattgatccacttcaaacagttcaacaatttcttcaaatgcatca
atctctcatgaactcaccatcagttccgacatgcctttcgaaacttttgatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTGACACCAGCACAACATTTATCC 238 297 39 Antisense
ACAACATTTATCCTCAACATCTACA 555 111 51 Antisense
CAACATCTACAGTTACATCTCCAAC 19 189 65 Antisense
GTTACATCTCCAACTAGTCAAAATA 146 447 76 Antisense
GTTTTCGACCTTTGATTGCTTCGGA 156 449 236 Antisense
GATTGCTTCGGAACTAAGATCTGGA 644 433 249 Antisense
GCGATTGGCAGGACCATCATCATCT 138 289 309 Antisense
GCATCAATCTCTCATGAACTCACCA 338 309 453 Antisense
ATGAACTCACCATCAGTTCCGACAT 401 43 466 Antisense
ATCAGTTCCGACATGCCTTTCGAAA 599 27 477 Antisense
GACATGCCTTTCGAAACTTTTGATC 303 369 486 Antisense
Sequence Source Affymetrix Proprietary Database