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[187828_at]

Description C24A11.8 /REP_DB=WormBase Gene ID /WP=CE19698 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:P91092 protein_id:AAD32269.1
Cluster 6grp_highest_10_10 > 48      6grp_high1_10_10 > 84      4grp_highest_8_8 > 55      4grp_high_8_8 > 19      6grp_highest_nonorm > 57      6grp_total > 75      4grp_total > 21      6grp_cgc > 66      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 661
1 2 3 4 range
A 1622.2
(-0.4)
P 		1816.5
(1.6)
P 		1355.5
(-3)
P 		1963.4
(3)
P 		608
B 1720.8
(3)
P 		1474.7
(-0.5)
P 		1302.3
(-3)
P 		1560.3
(0.7)
P 		419
C 1605.3
(0.6)
P 		1384.3
(-3)
P 		1458.3
(-1.8)
P 		1750
(3)
P 		366
avr 1649.4
(1.3) 		1558.5
(-0.1) 		1372
(-3) 		1757.9
(3) 		386

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1002
1 2 3 4 5 6 range
A 2398.7
(-0.5)
P 		2111.3
(-3)
P 		2165.4
(-2.5)
P 		2559.1
(0.9)
P 		2552.4
(0.8)
P 		2803.4
(3)
P 		692
B 2097.1
(-2.6)
P 		2026.1
(-3)
P 		3028.1
(3)
P 		2871.9
(2.1)
P 		2255.9
(-1.6)
P 		2815.5
(1.7)
P 		1002
C 2049.8
(-3)
P 		2277.4
(-1.4)
P 		2602.3
(0.8)
P 		2913.1
(3)
P 		2191.7
(-2)
P 		2690.5
(1.5)
P 		863
avr 2181.9
(-2.6) 		2138.3
(-3) 		2598.6
(1.3) 		2781.4
(3) 		2333.3
(-1.2) 		2769.8
(2.9) 		643
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 303
1 2 3 4 range
A 590.6
(3)
P 		394.3
(-0.9)
P 		354.4
(-1.7)
P 		287.4
(-3)
P 		303
B 548.8
(3)
P 		364.2
(-1.5)
P 		301.8
(-3)
P 		332.5
(-2.3)
P 		247
C 531.4
(3)
P 		398
(-0.4)
P 		508.3
(2.4)
P 		295.1
(-3)
P 		236
avr 556.9
(3) 		385.5
(-1.1) 		388.2
(-1) 		305
(-3) 		252

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 608
1 2 3 4 5 6 range
A 442.2
(-3)
P 		750.9
(2.6)
P 		471.6
(-2.5)
P 		649.1
(0.8)
P 		770.2
(3)
P 		682.5
(1.4)
P 		328
B 729.7
(-2.1)
P 		674.4
(-3)
P 		735.3
(-2)
P 		760.7
(-1.6)
P 		916.6
(0.9)
P 		1049.9
(3)
P 		376
C 759.8
(0.3)
P 		694.9
(-0.9)
P 		662.3
(-1.5)
P 		585.8
(-3)
P 		709.2
(-0.6)
P 		900.7
(3)
P 		315
avr 643.9
(-2.5) 		706.7
(-1) 		623.1
(-3) 		665.2
(-2) 		798.7
(1.1) 		877.7
(3) 		255
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187828_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.465
Sequence Type Exemplar sequence
Representative Public ID C24A11.8 NCBI
Target Description C24A11.8 /REP_DB=WormBase Gene ID /WP=CE19698 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:P91092 protein_id:AAD32269.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059185(11), NM_059186(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059185 NCBI Caenorhabditis elegans FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-4) (frm-4) mRNA, complete cds. 11/11 None
NM_059186 NCBI Caenorhabditis elegans FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-4) (frm-4) mRNA, complete cds. 11/11 None
SNAP00000029000 ENSEMBL cdna:SNAP chromosome:CEL140:I:5403324:5408341:-1 10/11 None
C24A11.8a ENSEMBL cdna:known chromosome:CEL140:I:5402898:5408347:-1 gene:C24A11.8 11/11 A 
C24A11.8b ENSEMBL cdna:known chromosome:CEL140:I:5403324:5408347:-1 gene:C24A11.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000029003 6/11 Cross Hyb Matching Probes None
GENEFINDER00000029004 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5403323-5408341(-) 100.0 100.0
Public Domain and Genome References
Ensembl C24A11.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAD32269 Ferm domain (protein4.1-ezrin-radixin-moesin) family protein 4, isoform a [Caenorhabditis elegans] ref|NP_491586.1| FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-4) [Caenorhabditis elegans] 0.0
blast AAK68201 Ferm domain (protein4.1-ezrin-radixin-moesin) family protein 4, isoform b [Caenorhabditis elegans] ref|NP_491587.1| FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-4) [Caenorhabditis elegans] 0.0
blast CAE66927 Hypothetical protein CBG12316 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000299 EMBL-EBI Band 4.1 2.6E-41
Pfam IPR000299 EMBL-EBI Band 4.1 2.7E-40
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491586.1 1 568-590
NP_491587.1 1 576-598
C24A11.8a 1 568-590
C24A11.8b 1 576-598
SNAP00000029000 1 600-622
Sequence
Target Sequence 
>C. ELEGANS:187828_AT
gatcttctacgacaaacgccacgtgttgagcagccatggctgaaacaaaattcactcaaa
gttactacagttcatgataatacagcttcatcatcatcatctccggacaattcggaaact
cagaacaataatctatcaaaaattacaaatttaaatctatcaattgagagagaagtgagt
acatccccatcgacatctcctgtgccaattgtccacactcaacagcattccgaccacaat
tcacaaaatcttgagaagaatggcaatggaaagttagccaatggaggtggatctggcgtt
gatttgggaaaaaaaactgtacacaccaatgggaccactctaatatgcacagaagagaag
caagactcaatattgagcatggatgagtctttgacatcgaagagtttgatttctcgaatt
gccaacacttgcttcgtattcttcctaatacttctgctcatcgttgccattgttattgtt
ctgtttgaaaaatcagaagactcggcacacaacgattttattgaaagtcatcgagcgtta
tcggacctcaggcatttgtactatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCTTCTACGACAAACGCCACGTG 177 401 1297 Antisense
ACGCCACGTGTTGAGCAGCCATGGC 157 91 1312 Antisense
GAGAGAAGTGAGTACATCCCCATCG 423 395 1464 Antisense
CAATTGTCCACACTCAACAGCATTC 504 179 1502 Antisense
GCATTCCGACCACAATTCACAAAAT 235 307 1521 Antisense
GTACACACCAATGGGACCACTCTAA 175 461 1615 Antisense
AATTGCCAACACTTGCTTCGTATTC 77 179 1713 Antisense
TATTCTTCCTAATACTTCTGCTCAT 592 661 1733 Antisense
TGCTCATCGTTGCCATTGTTATTGT 691 579 1751 Antisense
AGTCATCGAGCGTTATCGGACCTCA 124 61 1822 Antisense
TCGGACCTCAGGCATTTGTACTATG 165 607 1837 Antisense
Sequence Source Affymetrix Proprietary Database