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[187813_s_at]

Description Y54E10A.9 /REP_DB=WormBase Gene ID /WP=CE24451 /CHR=1 /FEA=Sanger Annotation /DEF=helicase (ST.LOUIS) protein_id:AAF60764.1
Cluster 6grp_total > 90      4grp_total > 34      6grp_cgc > 09      6grp_cgc_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 144
1 2 3 4 range
A 132.5
(-0)
A 		91.7
(-3)
A 		158.7
(1.9)
A 		173.6
(3)
A 		82
B 129.3
(-3)
A 		190.5
(3)
A 		163.4
(0.3)
A 		148.4
(-1.1)
P 		61
C 137
(-1.9)
A 		236
(3)
A 		113.7
(-3)
A 		147.3
(-1.4)
A 		122
avr 132.9
(-3) 		172.7
(3) 		145.3
(-1.1) 		156.4
(0.5) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 193
1 2 3 4 5 6 range
A 81.7
(-1.8)
A 		159.1
(2.4)
A 		59.6
(-3)
A 		138.1
(1.2)
A 		133.3
(1)
A 		170.5
(3)
A 		111
B 79
(-1)
A 		86.7
(-0.6)
A 		40.6
(-3)
A 		155.3
(3)
A 		97.4
(-0)
A 		115.4
(0.9)
A 		115
C 233.6
(3)
P 		95.1
(-2.8)
A 		90.9
(-3)
A 		123.9
(-1.6)
A 		157.2
(-0.2)
P 		133.3
(-1.2)
A 		143
avr 131.4
(2.3) 		113.6
(0.9) 		63.7
(-3) 		139.1
(3) 		129.3
(2.2) 		139.7
(3) 		76
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 48.2
(3)
A 		19.9
(-3)
A 		41.5
(1.6)
A 		25.4
(-1.8)
A 		28
B 41.2
(0.7)
A 		47
(3)
A 		37.9
(-0.5)
A 		31.6
(-3)
P 		15
C 45.4
(-0.1)
A 		67.8
(3)
A 		39.6
(-0.9)
A 		24.8
(-3)
A 		43
avr 44.9
(3) 		44.9
(3) 		39.7
(1.2) 		27.3
(-3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 77
1 2 3 4 5 6 range
A 15.1
(-2.7)
A 		56.6
(3)
A 		13
(-3)
A 		35
(0)
A 		40.2
(0.7)
A 		41.5
(0.9)
A 		44
B 27.5
(0.2)
A 		28.9
(0.4)
A 		9.9
(-3)
A 		41.1
(2.7)
A 		39.6
(2.4)
A 		43
(3)
A 		33
C 86.6
(3)
P 		29
(-2.4)
A 		23.1
(-3)
A 		24.9
(-2.8)
A 		50.9
(-0.4)
P 		44.6
(-1)
A 		64
avr 43.1
(2.9) 		38.2
(1.9) 		15.3
(-3) 		33.7
(0.9) 		43.6
(3) 		43
(2.9) 		28
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187813_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.260
Sequence Type Exemplar sequence
Representative Public ID Y54E10A.9 NCBI
Target Description Y54E10A.9 /REP_DB=WormBase Gene ID /WP=CE24451 /CHR=1 /FEA=Sanger Annotation /DEF=helicase (ST.LOUIS) protein_id:AAF60764.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058712(11), NM_058711(11), NM_001026622(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058712 NCBI Caenorhabditis elegans Vasa- and Belle-like Helicase family member (vbh-1) (vbh-1) mRNA, complete cds. 11/11 None
NM_058711 NCBI Caenorhabditis elegans Vasa- and Belle-like Helicase family member (vbh-1) (vbh-1) mRNA, complete cds. 11/11 None
NM_001026622 NCBI Caenorhabditis elegans Vasa- and Belle-like Helicase family member (vbh-1) (vbh-1) mRNA, complete cds. 11/11 None
SNAP00000033825 ENSEMBL cdna:SNAP chromosome:CEL140:I:3159765:3171152:-1 11/11 None
GENEFINDER00000033847 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3159765:3162284:-1 11/11 None
Y54E10A.9a ENSEMBL cdna:known chromosome:CEL140:I:3158515:3162372:-1 gene:Y54E10A.9 11/11 None
Y54E10A.9c ENSEMBL cdna:known chromosome:CEL140:I:3159765:3162136:-1 gene:Y54E10A.9 11/11 None
Y54E10A.9b ENSEMBL cdna:known chromosome:CEL140:I:3159765:3162375:-1 gene:Y54E10A.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3159764-3162284(-) 100.0 100.0
Public Domain and Genome References
Ensembl Y54E10A.9
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60764 Vasa- and belle-like helicase protein 1, isoform a [Caenorhabditis elegans] ref|NP_491113.1| Vasa- and Belle-like Helicase family member (vbh-1) [Caenorhabditis elegans] 0.0
blast AAK68520 Vasa- and belle-like helicase protein 1, isoform b [Caenorhabditis elegans] ref|NP_491112.1| Vasa- and Belle-like Helicase family member (vbh-1) [Caenorhabditis elegans] 0.0
blast AAU20831 Vasa- and belle-like helicase protein 1, isoform c [Caenorhabditis elegans] ref|NP_001021793.1| Vasa- and Belle-like Helicase family member (vbh-1) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 9.1E-38
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 1.6E-83
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 9.1E-38
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 1.6E-83
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 9.1E-38
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 1.6E-83
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 9.1E-38
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 1.6E-83
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 9.1E-38
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 1.6E-83
Sequence
Target Sequence 
>C. ELEGANS:187813_S_AT
tattccaaatgtgcgccacgtcatcaattatgatcttcctggagattctgacgaatatgt
tcaccgtatcggtagaaccggaagatgcggaaaccttggaattgccaccagtttcttcaa
tgacaagaatcgaggaatcggtcgtgatttgaagaatctgattgtcgaatccaaccaaga
agtcccagaatggcttcatcaagttgctgcagaaggacgcattggaggtggacaacgtgg
tggaaaccgtcgcttcggagcaaccgactatcgtgttggtcgtggtggtggtggatcgtt
cggaggaaacttctcaaattacaattccggtggagccactggtggtttcggaagtggacc
cgcctacggtggttcgttcggaggaggagcaccacgtcgcccgttcacgaatggaggatt
cggagctcatagctctctgtcgtctacctctaatagtggtggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATTCCAAATGTGCGCCACGTCATC 345 661 1344 Antisense
CTTGGAATTGCCACCAGTTTCTTCA 108 217 1438 Antisense
GAATGGCTTCATCAAGTTGCTGCAG 316 333 1531 Antisense
TGGTGGAAACCGTCGCTTCGGAGCA 494 551 1581 Antisense
GGAGCAACCGACTATCGTGTTGGTC 625 519 1600 Antisense
GGTGGTGGTGGATCGTTCGGAGGAA 569 499 1627 Antisense
CCGGTGGAGCCACTGGTGGTTTCGG 689 263 1670 Antisense
GGTTCGTTCGGAGGAGGAGCACCAC 25 507 1714 Antisense
CGTCGCCCGTTCACGAATGGAGGAT 7 245 1738 Antisense
GATTCGGAGCTCATAGCTCTCTGTC 303 433 1760 Antisense
GTCGTCTACCTCTAATAGTGGTGGC 315 473 1782 Antisense
Sequence Source Affymetrix Proprietary Database