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[187806_s_at]

Description K07E3.4A /REP_DB=WormBase Gene ID /WP=CE18020 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=tetrahydrofolate synthase
Cluster 6grp_highest_10_10 > 46      6grp_high1_10_10 > 64      4grp_highest_8_8 > 42      4grp_high_8_8 > 41      6grp_highest_nonorm > 36      6grp_total > 55      4grp_total > 54      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4175
1 2 3 4 range
A 5318.1
(3)
P 		2482.1
(-1.5)
P 		2993.9
(-0.7)
P 		1539.8
(-3)
P 		3778
B 4631
(3)
P 		2848.3
(-0.5)
P 		2485.1
(-1.2)
P 		1570.2
(-3)
P 		3061
C 4917.8
(3)
P 		2421.9
(-1)
P 		2567.4
(-0.7)
P 		1143.4
(-3)
P 		3774
avr 4955.6
(3) 		2584.1
(-1) 		2682.1
(-0.9) 		1417.8
(-3) 		3538

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2366
1 2 3 4 5 6 range
A 3547.2
(-0.8)
P 		4399.2
(2.4)
P 		4255.9
(1.9)
P 		3553.5
(-0.8)
P 		2958.6
(-3)
P 		4552.7
(3)
P 		1594
B 3531.1
(-0.5)
P 		3515.9
(-0.6)
P 		4256
(3)
P 		3476.6
(-0.8)
P 		3016.1
(-3)
P 		4014.9
(1.8)
P 		1240
C 3653.5
(-1.6)
P 		3266.6
(-2.6)
P 		5324.1
(3)
P 		3132.7
(-3)
P 		3834.2
(-1.1)
P 		4309
(0.2)
P 		2191
avr 3577.3
(-1.6) 		3727.2
(-1) 		4612
(3) 		3387.6
(-2.5) 		3269.6
(-3) 		4292.2
(1.6) 		1342
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1744
1 2 3 4 range
A 1936.3
(3)
P 		538.8
(-1.9)
P 		782.7
(-1)
P 		225.4
(-3)
P 		1711
B 1476.8
(3)
P 		703.5
(-1.1)
P 		575.9
(-1.7)
P 		334.6
(-3)
P 		1142
C 1627.9
(3)
P 		696.3
(-0.9)
P 		894.8
(-0.1)
P 		192.8
(-3)
P 		1435
avr 1680.3
(3) 		646.2
(-1.3) 		751.1
(-0.9) 		250.9
(-3) 		1429

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 935
1 2 3 4 5 6 range
A 653.9
(-3)
P 		1564.6
(3)
P 		926.8
(-1.2)
P 		901.3
(-1.4)
P 		892.8
(-1.4)
P 		1108.4
(-0)
P 		911
B 1228.7
(0.2)
P 		1170.3
(-0.4)
P 		1033.5
(-1.8)
P 		920.8
(-3)
P 		1225.4
(0.2)
P 		1497.2
(3)
P 		576
C 1354.2
(2.3)
P 		996.7
(-0.3)
P 		1355.1
(2.4)
P 		630
(-3)
P 		1240.7
(1.5)
P 		1442.5
(3)
P 		813
avr 1078.9
(-0.1) 		1243.9
(1.8) 		1105.1
(0.2) 		817.4
(-3) 		1119.6
(0.4) 		1349.4
(3) 		532
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187806_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16061
Sequence Type Exemplar sequence
Representative Public ID K07E3.4A NCBI
Target Description K07E3.4A /REP_DB=WormBase Gene ID /WP=CE18020 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=tetrahydrofolate synthase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076960(11), NM_076959(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076960 NCBI Caenorhabditis elegans K07E3.4a (K07E3.4) mRNA, complete cds. 11/11 None
NM_076959 NCBI Caenorhabditis elegans K07E3.4b (K07E3.4) mRNA, complete cds. 11/11 None
SNAP00000041514 ENSEMBL cdna:SNAP chromosome:CEL140:X:8080955:8086489:-1 11/11 None
GENEFINDER00000041526 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:8080955:8086489:-1 11/11 None
K07E3.4a ENSEMBL cdna:known chromosome:CEL140:X:8080812:8083342:-1 gene:K07E3.4 11/11 None
K07E3.4b ENSEMBL cdna:known chromosome:CEL140:X:8080955:8083958:-1 gene:K07E3.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8080953-8083221(-) 99.9 99.9
Public Domain and Genome References
Ensembl K07E3.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC69101 Hypothetical protein K07E3.4b [Caenorhabditis elegans] gb|AAC69104.1| Hypothetical protein K07E3.4a [Caenorhabditis elegans] ref|NP_509360.1| K07E3.4b [Caenorhabditis elegans] ref|NP_509361.1| K07E3.4a [Caenorhabditis elegans] 0.0
blast BAC40513.1 unnamed protein product [Mus musculus] gb|AAL99693.1| C1-tetrahydrofolate synthase [Mus musculus] gb|AAL99692.1| C1-tetrahydrofolate synthase [Mus musculus] 0.0
blast BAE35392.1 unnamed protein product [Mus musculus] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000559 EMBL-EBI Formate-tetrahydrofolate ligase, FTHFS 1.0E-126
Pfam IPR000559 EMBL-EBI Formate-tetrahydrofolate ligase, FTHFS 1.0E-126
Pfam IPR000672 EMBL-EBI Tetrahydrofolate dehydrogenase/cyclohydrolase 3.0E-66
Pfam IPR000672 EMBL-EBI Tetrahydrofolate dehydrogenase/cyclohydrolase 8.0E-103
Sequence
Target Sequence 
>C. ELEGANS:187806_S_AT
gaacaagttcgcaaccgataccgacaaggagttggaacttgtttgctccaaggccaagga
atacggagcatttgatgcagtggtttccgaacactggtcacaaggaggcgctggtgcggt
tgctctcgccaattctctggtcaatgctacaactggacatccaaagcaattcaaattcct
ttacaacctggatttgtcattggaagacaagatcgctactatcgccaaagaaatctatgg
tgctgatggcattgaactatctgacgaggccaaacagaaattggaaagatacacaagaca
gggattctcgaagttgccaatttgtatggcaaaaactcatttgtccctttcatcagaccc
aacaaagaagggtgctccaactggtttcacacttccgattcgcgacgtgcgtgcatccgt
tggagctggattcatttacccattggttggtgaaatgaccacaatgccaggactcaacac
tcgtccgtgcttctacgacatcaccattgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACAAGTTCGCAACCGATACCGAC 443 353 1392 Antisense
GGAACTTGTTTGCTCCAAGGCCAAG 341 527 1425 Antisense
GTGGTTTCCGAACACTGGTCACAAG 409 489 1471 Antisense
ACAAGGAGGCGCTGGTGCGGTTGCT 116 113 1491 Antisense
CTCTCGCCAATTCTCTGGTCAATGC 131 231 1514 Antisense
AGACAAGATCGCTACTATCGCCAAA 547 71 1596 Antisense
CAAAAACTCATTTGTCCCTTTCATC 458 191 1721 Antisense
AAGAAGGGTGCTCCAACTGGTTTCA 682 151 1756 Antisense
GGAGCTGGATTCATTTACCCATTGG 511 519 1813 Antisense
CACAATGCCAGGACTCAACACTCGT 492 193 1851 Antisense
GTGCTTCTACGACATCACCATTGAC 677 479 1878 Antisense
Sequence Source Affymetrix Proprietary Database