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[187776_s_at]

Description C06A5.3 /REP_DB=WormBase Gene ID /WP=CE07950 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:O01478 protein_id:AAB52437.1
Cluster 6grp_highest_10_10 > 66      6grp_high1_10_10 > 75      4grp_highest_8_8 > 31      4grp_high_8_8 > 13      6grp_highest_nonorm > 91      6grp_total > 66      4grp_total > 11      6grp_highest_sub > Da      6grp_cgc > 47      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 610
1 2 3 4 range
A 222.5
(-2)
P 		217.8
(-2.1)
P 		130.6
(-3)
P 		694.3
(3)
P 		564
B 219.9
(-1)
P 		171.6
(-1.7)
P 		84.2
(-3)
P 		493
(3)
P 		409
C 223.8
(-1.7)
P 		212.5
(-1.9)
P 		126.5
(-3)
P 		587.2
(3)
P 		461
avr 222.1
(-1.6) 		200.6
(-1.9) 		113.8
(-3) 		591.5
(3) 		478

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 218
1 2 3 4 5 6 range
A 227.8
(-1.3)
P 		201.7
(-2.7)
P 		261.1
(0.4)
P 		292.5
(1.9)
P 		195
(-3)
P 		313.3
(3)
P 		118
B 239.6
(-1.3)
P 		244.9
(-1.1)
P 		239.4
(-1.3)
P 		195.6
(-3)
P 		242.4
(-1.2)
P 		352.7
(3)
P 		157
C 190
(-2.2)
P 		206.9
(-1.8)
P 		211.5
(-1.7)
P 		319.6
(1.3)
P 		162.5
(-3)
P 		380.6
(3)
P 		218
avr 219.1
(-2.2) 		217.8
(-2.3) 		237.3
(-1.5) 		269.2
(-0.2) 		200
(-3) 		348.9
(3) 		149
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 81
(1.2)
P 		47.3
(-1.8)
P 		34.1
(-3)
P 		101.6
(3)
P 		68
B 70.1
(0.5)
P 		42.4
(-1.4)
P 		19.5
(-3)
P 		105.1
(3)
P 		86
C 74.1
(0.3)
P 		61.1
(-1.1)
P 		44.1
(-3)
P 		99
(3)
P 		55
avr 75.1
(0.7) 		50.3
(-1.5) 		32.6
(-3) 		101.9
(3) 		69

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 42
(-3)
P 		71.7
(2.2)
P 		56.9
(-0.4)
P 		74.2
(2.6)
P 		58.8
(-0.1)
P 		76.3
(3)
P 		34
B 83.4
(-0.6)
P 		81.5
(-0.8)
P 		58.1
(-2.5)
P 		51.8
(-3)
P 		98.5
(0.5)
P 		131.5
(3)
P 		80
C 70.4
(-1.6)
P 		63.1
(-2.2)
P 		53.8
(-2.9)
P 		64.3
(-2.1)
P 		52.6
(-3)
P 		127.4
(3)
P 		75
avr 65.3
(-2) 		72.1
(-1.3) 		56.3
(-3) 		63.4
(-2.2) 		70
(-1.5) 		111.7
(3) 		55
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187776_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.685
Sequence Type Exemplar sequence
Representative Public ID C06A5.3 NCBI
Target Description C06A5.3 /REP_DB=WormBase Gene ID /WP=CE07950 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:O01478 protein_id:AAB52437.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059332(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059332 NCBI Caenorhabditis elegans yeast SIR related family member (sir-2.4) (sir-2.4) mRNA, complete cds. 11/11 None
SNAP00000012787 ENSEMBL cdna:SNAP chromosome:CEL140:I:5998880:6000763:1 11/11 None
GENEFINDER00000012806 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5998880:6000763:1 10/11 None
C06A5.11 ENSEMBL cdna:known chromosome:CEL140:I:5999206:6000763:1 gene:C06A5.11 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5994191-6000763(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol sir-2.4
Ensembl C06A5.11
Entrez Gene 182284 Entrez gene
SwissProt Q95Q89 EMBL-EBI
WormBase CE27656 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6342 chromatin silencing inferred from electronic annotation QuickGO AmiGO
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5677 chromatin silencing complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK68163 Yeast sir related protein 2.4 [Caenorhabditis elegans] ref|NP_491733.1| yeast SIR related family member (sir-2.4) [Caenorhabditis elegans] 1.0E-171
blast T25520 hypothetical protein C06A5.3 - Caenorhabditis elegans 1.0E-154
blast AAK68163 Yeast sir related protein 2.4 [Caenorhabditis elegans] ref|NP_491733.1| yeast SIR related family member (sir-2.4) [Caenorhabditis elegans] 1.0E-154
blast AAK68163 Yeast sir related protein 2.4 [Caenorhabditis elegans] ref|NP_491733.1| yeast SIR related family member (sir-2.4) [Caenorhabditis elegans] 1.0E-152
blast T25520 hypothetical protein C06A5.3 - Caenorhabditis elegans 1.0E-152
blast CAE60330 Hypothetical protein CBG03922 [Caenorhabditis briggsae] 1.0E-144
Protein Domains
Database ID Description E-Value
Pfam IPR003000 EMBL-EBI Silent information regulator protein Sir2 5.7E-8
Pfam IPR003000 EMBL-EBI Silent information regulator protein Sir2 3.9E-9
Pfam IPR003000 EMBL-EBI Silent information regulator protein Sir2 5.7E-8
Sequence
Target Sequence 
>C. ELEGANS:187776_S_AT
gtggactttcaagttgctcgtcccggagtctcacacaaatcaatattggcacttcacaag
gcgggttacatcaaaacgataatcacacagaatgttgatggattggatcgaaaagtcggg
attccagttgaagatctcattgaagtacatggaaatttatttctagaagtctgtcaatca
tgttttagtgaatatgtacgagaggaaattgtaatgagtgttggcctgtgtccaactggt
cgtaattgtgaaggaaacaaacgaaccgggcgatcgtgtcgtggtaaacttcgtgatgca
actctcgattgggatacagaaatcagtttaaatcatttggacagaatccgaaaagcatgg
aaacaaacatctcatcttctttgtattggaacatcacttgaaattattccaatgggatct
ctaccacttgatgcaaaatcaaaaggaattaaaacaactacaattaattatcaggaaact
gcacatgaaaaaatcgtggaaactgcaattcatgctgacgttaaacttattctttactca
ttatgcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGGACTTTCAAGTTGCTCGTCCCG 191 491 2680 Antisense
GTCCCGGAGTCTCACACAAATCAAT 528 473 2699 Antisense
GGCACTTCACAAGGCGGGTTACATC 449 523 2727 Antisense
TTGTAATGAGTGTTGGCCTGTGTCC 657 707 2888 Antisense
GCCTGTGTCCAACTGGTCGTAATTG 443 281 2903 Antisense
AAACGAACCGGGCGATCGTGTCGTG 450 125 2938 Antisense
GTGTCGTGGTAAACTTCGTGATGCA 269 487 2955 Antisense
GTGATGCAACTCTCGATTGGGATAC 603 483 2972 Antisense
TGGAAACAAACATCTCATCTTCTTT 517 547 3037 Antisense
TGGGATCTCTACCACTTGATGCAAA 68 555 3092 Antisense
AACTTATTCTTTACTCATTATGCAA 545 139 3203 Antisense
Sequence Source Affymetrix Proprietary Database