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[187775_at]

Description F40B5.2 /REP_DB=WormBase Gene ID /WP=CE01936 /CHR=X /FEA=Sanger Annotation /DEF=(ST.LOUIS) SW:Q09320 protein_id:AAA64337.1
Cluster 6grp_high1_10_10 > 91      6grp_total > 92      4grp_total > 17      6grp_high1_sub > 05      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 40.5
(-3)
P 		52.9
(-1.7)
A 		48.3
(-2.2)
A 		96.9
(3)
P 		56
B 29.8
(-3)
A 		61.9
(1.8)
M 		30.6
(-2.9)
P 		70.3
(3)
P 		41
C 39.6
(-1.5)
A 		27.2
(-3)
A 		46.8
(-0.7)
P 		77.8
(3)
P 		51
avr 36.6
(-3) 		47.3
(-1.6) 		41.9
(-2.3) 		81.7
(3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 32.2
(-2.1)
A 		24.1
(-3)
A 		57.6
(0.8)
P 		44.3
(-0.7)
P 		77.1
(3)
P 		45.9
(-0.5)
A 		53
B 40.6
(-0.8)
A 		33.6
(-3)
P 		35.3
(-2.5)
A 		47.4
(1.4)
P 		52.6
(3)
P 		34.2
(-2.8)
P 		19
C 41.4
(0.3)
P 		56.7
(3)
P 		38.9
(-0.1)
A 		38.1
(-0.3)
A 		37.2
(-0.4)
A 		22.6
(-3)
M 		34
avr 38.1
(-1.9) 		38.1
(-1.9) 		43.9
(-0.3) 		43.3
(-0.4) 		55.6
(3) 		34.2
(-3) 		21
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 14.7
(3)
P 		11.5
(-3)
A 		12.6
(-0.9)
A 		14.2
(2.1)
P 		3
B 9.5
(-1.2)
A 		15.3
(3)
M 		7.1
(-3)
P 		15
(2.8)
P 		8
C 13.1
(0.7)
A 		7.8
(-3)
A 		16.3
(3)
P 		13.1
(0.7)
P 		9
avr 12.4
(-0.9) 		11.5
(-3) 		12
(-1.8) 		14.1
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 5.9
(-3)
A 		8.6
(-2.1)
A 		12.6
(-0.7)
P 		11.2
(-1.2)
P 		23.3
(3)
P 		11.2
(-1.2)
A 		17
B 14.1
(-0.4)
A 		11.2
(-1.8)
P 		8.6
(-3)
A 		12.6
(-1.1)
P 		21.4
(3)
P 		12.8
(-1)
P 		13
C 15.4
(1.8)
P 		17.3
(3)
P 		9.9
(-1.6)
A 		7.7
(-2.9)
A 		12
(-0.3)
A 		7.6
(-3)
M 		10
avr 11.8
(-2) 		12.4
(-1.6) 		10.4
(-3) 		10.5
(-2.9) 		18.9
(3) 		10.5
(-2.9) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187775_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16333
Sequence Type Exemplar sequence
Representative Public ID F40B5.2 NCBI
Target Description F40B5.2 /REP_DB=WormBase Gene ID /WP=CE01936 /CHR=X /FEA=Sanger Annotation /DEF=(ST.LOUIS) SW:Q09320 protein_id:AAA64337.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077018(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077018 NCBI Caenorhabditis elegans F40B5.3 (F40B5.3) mRNA, complete cds. 9/11 None
GENEFINDER00000013915 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:8372162:8374411:1 9/11 None
F40B5.3 ENSEMBL cdna:known chromosome:CEL140:X:8372162:8374411:1 gene:F40B5.3 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_077016 2/11 Cross Hyb Matching Probes None
NM_077017 2/11 Cross Hyb Matching Probes None
GENEFINDER00000013913 2/11 Cross Hyb Matching Probes None
SNAP00000013911 2/11 Cross Hyb Matching Probes None
F40B5.2a 2/11 Cross Hyb Matching Probes None
F40B5.2b 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8367795-8374651(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F40B5.3
Entrez Gene 185513 Entrez gene
SwissProt Q95ZU0 EMBL-EBI
WormBase CE28315 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4245 neprilysin activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK70642 Hypothetical protein F40B5.3 [Caenorhabditis elegans] ref|NP_509419.1| F40B5.3 [Caenorhabditis elegans] 1.0E-147
blast AAK70642 Hypothetical protein F40B5.3 [Caenorhabditis elegans] ref|NP_509419.1| F40B5.3 [Caenorhabditis elegans] 1.0E-123
blast CAE74890 Hypothetical protein CBG22755 [Caenorhabditis briggsae] 1.0E-63
blast CAE74890 Hypothetical protein CBG22755 [Caenorhabditis briggsae] 2.0E-47
blast CAE65803 Hypothetical protein CBG10911 [Caenorhabditis briggsae] 6.0E-23
blast AAC48280 Hypothetical protein C53B7.7 [Caenorhabditis elegans] ref|NP_509156.2| C53B7.7 [Caenorhabditis elegans] 5.0E-22
Protein Domains
Database ID Description E-Value
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 7.7E-18
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 7.7E-18
Sequence
Target Sequence 
>C. ELEGANS:187775_AT
ctgtcaggtcatacgcacggagggcaaatgtacccatttgtgccaattgtgcatcttgca
aatgcatttgtaagaggtcaatattatgacaaatcaaccgatacatacgtgtatgtaagt
gccggagttaattattttggaccgcctatcaaaatatacgttaacaaaagtgtgtgcccc
tgtgagaatttctataggcatgcatgctcgtttgatagcccacgcaatttaatggcaact
gcactcaaaaacttgacttatgaactcagacaaaagcaagcagacttgttctggaatcat
atcacgcttgtttccgactacaatcagaaatgggcgccgcaaaaagatctgatcgatagt
catgctgcaatggaaagaattgagcgccttaatgtaacccaggagcagttgtttttctat
tcatttgctaatcagttttgttccgggagtttatcaaaagttttcatagaggaagaggga
gactatgatccccattcagtaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTGTCAGGTCATACGCACGGAGGGC 661 239 1264 Antisense
AATGTACCCATTTGTGCCAATTGTG 20 165 1290 Antisense
TGTGTGCCCCTGTGAGAATTTCTAT 508 555 1434 Antisense
GCATGCATGCTCGTTTGATAGCCCA 91 311 1461 Antisense
GTTCTGGAATCATATCACGCTTGTT 314 443 1551 Antisense
ATGGGCGCCGCAAAAAGATCTGATC 123 49 1593 Antisense
GATCTGATCGATAGTCATGCTGCAA 390 419 1609 Antisense
AATTGAGCGCCTTAATGTAACCCAG 650 177 1641 Antisense
GGAGCAGTTGTTTTTCTATTCATTT 334 521 1665 Antisense
GCTAATCAGTTTTGTTCCGGGAGTT 181 297 1690 Antisense
GAGACTATGATCCCCATTCAGTAAA 209 393 1742 Antisense
Sequence Source Affymetrix Proprietary Database