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[187770_s_at]

Description F25B5.3 /REP_DB=WormBase Gene ID /WP=CE02676 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) SW:Q09315 protein_id:AAC46529.1
Cluster 6grp_highest_10_10 > 68      6grp_high1_10_10 > 86      4grp_highest_8_8 > 14      4grp_high_8_8 > 22      6grp_highest_nonorm > 89      6grp_total > 77      4grp_total > 09      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6074
1 2 3 4 range
A 1321
(-3)
P 		2373.5
(-1.9)
P 		2749.2
(-1.5)
P 		7121.8
(3)
P 		5801
B 1310.7
(-3)
P 		2197.9
(-2)
P 		3089.9
(-1)
P 		6519.8
(3)
P 		5209
C 1390.7
(-3)
P 		2297
(-2.1)
P 		3013.5
(-1.4)
P 		7384.5
(3)
P 		5994
avr 1340.8
(-3) 		2289.5
(-2) 		2950.9
(-1.3) 		7008.7
(3) 		5668

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1848
1 2 3 4 5 6 range
A 1063.2
(-3)
P 		1344.5
(-1.7)
P 		1100
(-2.8)
P 		1437.8
(-1.2)
P 		1682.2
(-0.1)
P 		2322.4
(3)
P 		1259
B 881.4
(-3)
P 		1250.9
(-1.2)
P 		1257.4
(-1.2)
P 		1490.6
(-0.1)
P 		1665.4
(0.7)
P 		2144.2
(3)
P 		1263
C 928.5
(-3)
P 		1212.8
(-2.1)
P 		1652
(-0.6)
P 		1230.2
(-2)
P 		1425.9
(-1.3)
P 		2729.5
(3)
P 		1801
avr 957.7
(-3) 		1269.4
(-1.7) 		1336.5
(-1.4) 		1386.2
(-1.2) 		1591.2
(-0.4) 		2398.7
(3) 		1441
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 971
1 2 3 4 range
A 481
(-3)
P 		515.2
(-2.6)
P 		718.7
(-0.5)
P 		1042.5
(3)
P 		562
B 418
(-3)
P 		542.8
(-2.2)
P 		716
(-1.2)
P 		1389.4
(3)
P 		971
C 460.4
(-3)
P 		660.4
(-1.5)
P 		1050.3
(1.5)
P 		1245.1
(3)
P 		785
avr 453.1
(-3) 		572.8
(-2.1) 		828.3
(-0.1) 		1225.7
(3) 		773

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 718
1 2 3 4 5 6 range
A 196
(-3)
P 		478.2
(1.6)
P 		239.6
(-2.3)
P 		364.7
(-0.3)
P 		507.6
(2.1)
P 		565.4
(3)
P 		369
B 306.7
(-3)
P 		416.4
(-1.7)
P 		305.3
(-3)
P 		394.8
(-1.9)
P 		676.6
(1.5)
P 		799.5
(3)
P 		494
C 344.2
(-2.1)
P 		370.1
(-1.9)
P 		420.5
(-1.4)
P 		247.4
(-3)
P 		461.4
(-1.1)
P 		913.7
(3)
P 		666
avr 282.3
(-3) 		421.6
(-1.2) 		321.8
(-2.5) 		335.6
(-2.3) 		548.5
(0.3) 		759.5
(3) 		477
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187770_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6738
Sequence Type Exemplar sequence
Representative Public ID F25B5.3 NCBI
Target Description F25B5.3 /REP_DB=WormBase Gene ID /WP=CE02676 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS) SW:Q09315 protein_id:AAC46529.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065892(11), NM_171142(11), NM_065893(11), NM_171141(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065892 NCBI Caenorhabditis elegans F25B5.3b (F25B5.3) mRNA, complete cds. 11/11 None
NM_171142 NCBI Caenorhabditis elegans F25B5.3c (F25B5.3) mRNA, complete cds. 11/11 None
NM_065893 NCBI Caenorhabditis elegans F25B5.3a (F25B5.3) mRNA, complete cds. 11/11 None
NM_171141 NCBI Caenorhabditis elegans F25B5.3d (F25B5.3) mRNA, complete cds. 11/11 None
GENEFINDER00000007792 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5948848:5953167:1 11/11 None
SNAP00000007785 ENSEMBL cdna:SNAP chromosome:CEL140:III:5951968:5953167:1 11/11 None
F25B5.3b ENSEMBL cdna:known chromosome:CEL140:III:5948837:5953470:1 gene:F25B5.3 11/11 None
F25B5.3c.1 ENSEMBL cdna:known chromosome:CEL140:III:5950081:5953167:1 gene:F25B5.3 11/11 None
F25B5.3a ENSEMBL cdna:known chromosome:CEL140:III:5950374:5953167:1 gene:F25B5.3 11/11 None
F25B5.3c.2 ENSEMBL cdna:known chromosome:CEL140:III:5951825:5953167:1 gene:F25B5.3 11/11 None
F25B5.3d ENSEMBL cdna:known chromosome:CEL140:III:5951834:5953167:1 gene:F25B5.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
F25B5.3e 1/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175843 5 4 NM_065892 187770_s_at (11)
NM_065893 187770_s_at (11)
NM_171141 187770_s_at (11)
NM_171142 187770_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5951894-5953166(+) 100.0 100.0
Public Domain and Genome References
Ensembl F25B5.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_498293 F25B5.3b [Caenorhabditis elegans] gb|AAK67228.1| Hypothetical protein F25B5.3b [Caenorhabditis elegans] sp|Q09315|YQT3_CAEEL Hypothetical protein F25B5.3 in chromosome III 0.0
blast NP_498294 F25B5.3a [Caenorhabditis elegans] gb|AAK67227.1| Hypothetical protein F25B5.3a [Caenorhabditis elegans] 0.0
blast T16147 hypothetical protein F25B5.3 - Caenorhabditis elegans 0.0
blast NP_741161 F25B5.3d [Caenorhabditis elegans] gb|AAM22066.1| Hypothetical protein F25B5.3d [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006434 EMBL-EBI HAD-superfamily subfamily IE hydrolase 1.0E-126
Pfam IPR006434 EMBL-EBI HAD-superfamily subfamily IE hydrolase 1.0E-126
Pfam IPR006434 EMBL-EBI HAD-superfamily subfamily IE hydrolase 1.0E-126
Pfam IPR006434 EMBL-EBI HAD-superfamily subfamily IE hydrolase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:187770_S_AT
agttctcgccgaatctgacgatggaggaaaagattccacatatggagaaatggtggggaa
caagtcattcattgattgtcaacgaaaagttctcaaagaacacaattgaagactttgttc
gtcaatcgagaattgttttcaaagatggagctgaagattttattgaagctctcgatgcac
acaacattccacttgtcatcttcagtgcaggaatcggaaacatcattgagtatttccttc
agcagaagctcggcgcaattccacgcaacacacatttcatctccaacatgattctgttcg
atgaggatgacaacgcttgtgcattctccgagccgttgatccacactttctgcaagaatt
catcagttattcagaaggaaacatcgtttttccatgatattgctggaagagtaaacgtaa
ttctgctcggtgactcgatgggtgatattcacatggacgttggagttgaaagagacgggc
ccacgttgaaagttggatattacaatggatcgcttgacgacaccgccgctctccagcact
atgaggaagtctacgacattgtcctgattcatgatccgacgttgaatgtggctca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTTCTCGCCGAATCTGACGATGGA 524 63 515 Antisense
GATGCACACAACATTCCACTTGTCA 451 413 688 Antisense
TTCAGCAGAAGCTCGGCGCAATTCC 558 683 752 Antisense
ACACACATTTCATCTCCAACATGAT 403 111 782 Antisense
GGATGACAACGCTTGTGCATTCTCC 399 513 819 Antisense
CGTTGATCCACACTTTCTGCAAGAA 13 249 848 Antisense
AATTCTGCTCGGTGACTCGATGGGT 369 177 933 Antisense
GGAGTTGAAAGAGACGGGCCCACGT 147 403 976 Antisense
CCGCTCTCCAGCACTATGAGGAAGT 141 263 1040 Antisense
GACATTGTCCTGATTCATGATCCGA 410 371 1069 Antisense
ATGATCCGACGTTGAATGTGGCTCA 527 45 1085 Antisense
Sequence Source Affymetrix Proprietary Database