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[187767_at]

Description F14B8.5 /REP_DB=WormBase Gene ID /WP=CE02648 /CHR=X /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:Q19445 protein_id:AAA68275.1
Cluster 6grp_highest_10_10 > 20      6grp_high1_10_10 > 14      4grp_highest_8_8 > 26      4grp_high_8_8 > 58      6grp_highest_nonorm > 90      6grp_total > 03      4grp_total > 60      6grp_highest_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 194
1 2 3 4 range
A 183.2
(1.5)
P 		138.5
(-0.8)
P 		211.5
(3)
P 		94.7
(-3)
P 		117
B 198.6
(3)
P 		141.5
(0.6)
P 		187.6
(2.5)
P 		57.2
(-3)
P 		141
C 192.1
(0.8)
P 		162
(-0.4)
P 		251
(3)
P 		92.6
(-3)
P 		158
avr 191.3
(1.9) 		147.3
(-0.1) 		216.7
(3) 		81.5
(-3) 		135

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 155
1 2 3 4 5 6 range
A 156.5
(2)
P 		175.1
(3)
P 		144.6
(1.4)
P 		146.4
(1.5)
P 		93.9
(-1.2)
P 		59.6
(-3)
P 		116
B 214.4
(3)
P 		178.6
(1)
P 		104.4
(-3)
P 		147.6
(-0.6)
P 		167.7
(0.5)
P 		108.6
(-2.8)
P 		110
C 166.9
(1.2)
P 		200.2
(3)
P 		122.4
(-1.1)
P 		171.6
(1.5)
P 		114.7
(-1.5)
P 		87.3
(-3)
P 		113
avr 179.3
(2.7) 		184.6
(3) 		123.8
(-0.7) 		155.2
(1.2) 		125.4
(-0.6) 		85.2
(-3) 		99
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 66.7
(3)
P 		30.1
(-1.2)
P 		55.3
(1.7)
P 		13.9
(-3)
P 		53
B 63.3
(3)
P 		34.9
(-0.3)
P 		43.5
(0.7)
P 		12.2
(-3)
P 		51
C 63.6
(1)
P 		46.6
(-0.4)
P 		87.5
(3)
P 		15.6
(-3)
P 		72
avr 64.5
(3) 		37.2
(-0.2) 		62.1
(2.7) 		13.9
(-3) 		51

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 60
1 2 3 4 5 6 range
A 28.9
(-1.2)
P 		62.3
(3)
P 		31.5
(-0.9)
P 		37.1
(-0.2)
P 		28.3
(-1.3)
P 		14.5
(-3)
P 		48
B 74.6
(3)
P 		59.4
(1.2)
P 		25.3
(-3)
P 		39.1
(-1.3)
P 		68.1
(2.2)
P 		40.5
(-1.2)
P 		49
C 61.8
(3)
P 		61.1
(2.9)
P 		31.2
(-2.6)
P 		34.5
(-2)
P 		37.1
(-1.5)
P 		29.2
(-3)
P 		33
avr 55.1
(1.9) 		60.9
(3) 		29.3
(-2.8) 		36.9
(-1.4) 		44.5
(0) 		28.1
(-3) 		33
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187767_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15964
Sequence Type Exemplar sequence
Representative Public ID F14B8.5 NCBI
Target Description F14B8.5 /REP_DB=WormBase Gene ID /WP=CE02648 /CHR=X /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:Q19445 protein_id:AAA68275.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076769(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076769 NCBI Caenorhabditis elegans F14B8.7 (F14B8.7) mRNA, complete cds. 11/11 None
SNAP00000027541 ENSEMBL cdna:SNAP chromosome:CEL140:X:6921672:6924403:-1 11/11 None
GENEFINDER00000027547 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:6921672:6924403:-1 11/11 None
F14B8.7 ENSEMBL cdna:known chromosome:CEL140:X:6921619:6924403:-1 gene:F14B8.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6921807-6928599(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F14B8.7
Entrez Gene 259710 Entrez gene
SwissProt Q95QL0 EMBL-EBI
WormBase CE29267 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL02486 Hypothetical protein F14B8.7 [Caenorhabditis elegans] ref|NP_509170.1| F14B8.7 [Caenorhabditis elegans] 0.0
blast CAE65819 Hypothetical protein CBG10933 [Caenorhabditis briggsae] 0.0
blast T16073 hypothetical protein F14B8.5 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004878 EMBL-EBI Protein of unknown function DUF270 1.3E-34
Pfam IPR004878 EMBL-EBI Protein of unknown function DUF270 5.0E-79
Pfam IPR004878 EMBL-EBI Protein of unknown function DUF270 1.3E-20
Pfam IPR004878 EMBL-EBI Protein of unknown function DUF270 5.0E-79
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509170.1 12 46-67,87-109,147-169,179-201,214-236,280-302,333-352,362-384,408-430,440-462,483-500,515-537
F14B8.7 12 46-67,87-109,147-169,179-201,214-236,280-302,333-352,362-384,408-430,440-462,483-500,515-537
SNAP00000027541 9 48-65,78-100,144-166,197-216,226-248,272-294,304-326,347-364,379-401
GENEFINDER00000027547 12 46-67,87-109,147-169,179-201,214-236,280-302,333-352,362-384,408-430,440-462,483-500,515-537
Sequence
Target Sequence 
>C. ELEGANS:187767_AT
tctgggctaatcttgaacgtctcaaacgagaacagatggaaaagaagatgaaaaaacgta
gcattttgaaaattgactgttctagaactgcagaaggtctgtttgccggattcgcttgta
ttattgtgactatcattgcaatagctctttttaatgcatattcttccgataagaatgtag
cgcaatggatcttctcatgcaccaacatgtccttcttccttgtttctactttcttggtcg
tttttgctttctggagaatgcgatatttgaagtttttgatggaagatgatgacgaagaag
atgacaatgcagaactgcttgatcgaattcttctagttgttggattgattggagaactca
tattctctattggtggaatcctatcatttatcaacaacatgaccatcggcctcccactta
tcatattcactactaacgtcttgagattggttattaccatgctgctcatgatgaattgtg
ctcaatttatgatgaacgtgtttgaagctcaaaaagctggaattaatgatgagatgatac
acatgtacgggtcgtactactgggcaatcgttgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTGGGCTAATCTTGAACGTCTCAA 476 607 2327 Antisense
TGCAGAAGGTCTGTTTGCCGGATTC 85 575 2415 Antisense
TGCCGGATTCGCTTGTATTATTGTG 164 585 2430 Antisense
TGTAGCGCAATGGATCTTCTCATGC 604 557 2502 Antisense
TTCTCATGCACCAACATGTCCTTCT 143 693 2518 Antisense
TCCTTGTTTCTACTTTCTTGGTCGT 115 591 2543 Antisense
TGGTCGTTTTTGCTTTCTGGAGAAT 118 549 2561 Antisense
GAACTGCTTGATCGAATTCTTCTAG 295 349 2638 Antisense
TATCAACAACATGACCATCGGCCTC 239 659 2715 Antisense
CTCCCACTTATCATATTCACTACTA 595 233 2737 Antisense
GTCGTACTACTGGGCAATCGTTGTC 335 471 2877 Antisense
Sequence Source Affymetrix Proprietary Database