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[187765_at]

Description C25E10.12 /REP_DB=WormBase Gene ID /WP=CE06874 /TR=SW:Q18161 /GB=AAA92317.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 28      6grp_high1_10_10 > 81      4grp_highest_8_8 > 38      4grp_high_8_8 > 03      6grp_highest_nonorm > 55      6grp_total > 81      4grp_total > 13      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 636
1 2 3 4 range
A 956.5
(-0)
P 		816
(-2)
P 		741.6
(-3)
P 		1176.2
(3)
P 		435
B 1055.3
(-0.3)
P 		835.8
(-2.5)
P 		786.4
(-3)
P 		1377.1
(3)
P 		591
C 1019
(0.1)
P 		824.8
(-3)
P 		862.7
(-2.4)
P 		1202.1
(3)
P 		377
avr 1010.3
(-0.2) 		825.5
(-2.6) 		796.9
(-3) 		1251.8
(3) 		455

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2279
1 2 3 4 5 6 range
A 1076.2
(-1.6)
P 		1176
(-1.2)
P 		1530.7
(-0.1)
P 		2517.8
(3)
P 		1899.4
(1)
P 		617.6
(-3)
P 		1900
B 954.6
(-2.4)
P 		1200.3
(-1.4)
P 		1495.6
(-0.1)
P 		2038.2
(2.2)
P 		2224.6
(3)
P 		817.2
(-3)
P 		1407
C 949
(-3)
P 		1151.7
(-2.4)
P 		1620.8
(-0.9)
P 		2896.2
(3)
P 		1822.6
(-0.3)
P 		1054.9
(-2.7)
P 		1947
avr 993.3
(-2.4) 		1176
(-1.7) 		1549
(-0.4) 		2484.1
(3) 		1982.2
(1.2) 		829.9
(-3) 		1654
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 176
1 2 3 4 range
A 348.3
(3)
P 		177.1
(-2.8)
P 		193.9
(-2.3)
P 		172.2
(-3)
P 		176
B 336.5
(3)
P 		206.4
(-2.1)
P 		182.2
(-3)
P 		293.5
(1.3)
P 		154
C 337.3
(3)
P 		237.1
(-1.5)
P 		300.7
(1.4)
P 		202.7
(-3)
P 		135
avr 340.7
(3) 		206.9
(-3) 		225.6
(-2.2) 		222.8
(-2.3) 		134

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 753
1 2 3 4 5 6 range
A 198.4
(-2.4)
P 		418.2
(0.3)
P 		333.3
(-0.8)
P 		638.6
(3)
P 		573.2
(2.2)
P 		150.4
(-3)
P 		488
B 332.1
(-2.7)
P 		399.5
(-2.1)
P 		363.2
(-2.4)
P 		539.8
(-0.6)
P 		903.8
(3)
P 		304.7
(-3)
P 		599
C 351.7
(-3)
P 		351.4
(-3)
P 		412.5
(-1.5)
P 		582.4
(2.8)
P 		589.8
(3)
P 		353.1
(-3)
P 		238
avr 294.1
(-2.6) 		389.7
(-1.3) 		369.7
(-1.6) 		586.9
(1.5) 		688.9
(3) 		269.4
(-3) 		420
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187765_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11349
Sequence Type Exemplar sequence
Representative Public ID C25E10.12 NCBI
Target Description C25E10.12 /REP_DB=WormBase Gene ID /WP=CE06874 /TR=SW:Q18161 /GB=AAA92317.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072948(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072948 NCBI Caenorhabditis elegans C25E10.12 (C25E10.12) mRNA, complete cds. 11/11 None
SNAP00000038743 ENSEMBL cdna:SNAP chromosome:CEL140:V:9063332:9065027:-1 11/11 None
GENEFINDER00000038746 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9063332:9065027:-1 11/11 None
C25E10.12 ENSEMBL cdna:known chromosome:CEL140:V:9063081:9065027:-1 gene:C25E10.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9063332-9065028(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C25E10.12
Entrez Gene 182896 Entrez gene
SwissProt Q18161 EMBL-EBI
WormBase CE06874 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.2267.1.S1_AT similar to CG16717-PA cfa
DROSOPHILA_2:1624186_AT dm
DROSGENOME1:148483_AT dm
HG-U133_PLUS_2:206436_AT metallophosphoesterase domain containing 1 hs
HG-FOCUS:206436_AT metallophosphoesterase domain containing 1 hs
HG-U133A_2:206436_AT metallophosphoesterase domain containing 1 hs
HG-U133A:206436_AT metallophosphoesterase domain containing 1 hs
U133_X3P:G4502496_3P_AT metallophosphoesterase domain containing 1 hs
HG-U95AV2:38215_AT metallophosphoesterase domain containing 1 hs
HG-U95C:64754_S_AT metallophosphoesterase domain containing 1 hs
HG-U95E:78306_AT metallophosphoesterase domain containing 1 hs
U133_X3P:HS2.406924.1.S1_3P_X_AT Metallophosphoesterase domain containing 1 hs
U133_X3P:HS2.375819.1.S1_3P_AT Metallophosphoesterase domain containing 1 hs
U133_X3P:HS2.350563.1.S1_3P_AT Metallophosphoesterase domain containing 1 hs
U133_X3P:HS2.348632.1.S1_3P_X_AT Metallophosphoesterase domain containing 1 hs
U133_X3P:1564773_3P_X_AT Metallophosphoesterase domain containing 1 hs
U133_X3P:1564721_3P_AT Metallophosphoesterase domain containing 1 hs
HG-U133_PLUS_2:1563106_AT Metallophosphoesterase domain containing 1 hs
HG-U133_PLUS_2:1564721_AT Metallophosphoesterase domain containing 1 hs
HG-U133_PLUS_2:1564749_AT Metallophosphoesterase domain containing 1 hs
HG-U133_PLUS_2:1564773_X_AT Metallophosphoesterase domain containing 1 hs
HG-U133_PLUS_2:241398_AT Metallophosphoesterase domain containing 1 hs
HG-U133B:241398_AT Metallophosphoesterase domain containing 1 hs
HU35KSUBA:D52609_S_AT Metallophosphoesterase domain containing 1 hs
U133_X3P:HS.82748.0.A1_3P_AT Metallophosphoesterase domain containing 1 hs
MOE430B:1433701_AT metallophosphoesterase domain containing 1 mm
MOUSE430_2:1433701_AT metallophosphoesterase domain containing 1 mm
MG-U74BV2:106829_AT DNA segment, Chr 15, Brigham & Women's Genetics 0669 expressed, mRNA (cDNA clone MGC:66572 IMAGE:6810744) mm
MU19KSUBA:TC15709_AT DNA segment, Chr 15, Brigham & Women's Genetics 0669 expressed, mRNA (cDNA clone MGC:66572 IMAGE:6810744) mm
RAE230B:1396924_AT Similar to bM150J22.1 (novel protein (ortholog of human C22orf1)) (predicted) rn
RAT230_2:1396924_AT Similar to bM150J22.1 (novel protein (ortholog of human C22orf1)) (predicted) rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA92317 Hypothetical protein C25E10.12 [Caenorhabditis elegans] ref|NP_505349.1| C25E10.12 [Caenorhabditis elegans] sp|Q18161|YBPT_CAEEL Hypothetical UPF0046 protein C25E10.12 in chromosome V 1.0E-170
blast CAE72089 Hypothetical protein CBG19177 [Caenorhabditis briggsae] 1.0E-168
blast EAA09732 ENSANGP00000012720 [Anopheles gambiae str. PEST] ref|XP_314287.2| ENSANGP00000012720 [Anopheles gambiae str. PEST] 5.0E-68
Protein Domains
Database ID Description E-Value
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 2.0E-14
Sequence
Target Sequence 
>C. ELEGANS:187765_AT
ttggatccctgccacacaaacataaaatagtaattgctggaaatcatgagctcggttttg
aagacggtgaagaaatgagtgaacgtcaactggcaggccttaacatgctcggaatcaata
aagcctatgaacttctctcgaattgtacatacctctgtgataaatcatatgaagcatatg
gactcaaaatatatggagcaccatggcactcgatgccaggatactcatttttccgtcaga
gaggacaaaaaattcttcacaaatggaatcaaatccccgcgaaaattgatgttctaatga
cccatacacctccacttggacatggtgattttaatgcctgggacaaaatggatggaatcc
tttgtggatgtgcagaacttttgaacactgtcgagcaacgtgtaaaaccaaaatatcacg
tgtttggacacgttcatcaaaagcatggtgtgactacgaatggagaaacaacattcatca
atgcagcactttgtgatcataagcttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGGATCCCTGCCACACAAACATAA 324 709 290 Antisense
TGAGTGAACGTCAACTGGCAGGCCT 72 569 365 Antisense
TGGCAGGCCTTAACATGCTCGGAAT 219 545 380 Antisense
AAAGCCTATGAACTTCTCTCGAATT 479 125 409 Antisense
CTCTCGAATTGTACATACCTCTGTG 390 231 424 Antisense
TGGAGCACCATGGCACTCGATGCCA 196 547 483 Antisense
GCCAGGATACTCATTTTTCCGTCAG 66 277 504 Antisense
ATGGAATCAAATCCCCGCGAAAATT 48 51 552 Antisense
GATGTTCTAATGACCCATACACCTC 104 411 577 Antisense
GAACTTTTGAACACTGTCGAGCAAC 131 351 664 Antisense
GCAGCACTTTGTGATCATAAGCTTC 397 313 772 Antisense
Sequence Source Affymetrix Proprietary Database