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[187757_s_at]

Description F56B3.2 /REP_DB=WormBase Gene ID /WP=CE17883 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:O45113 protein_id:AAC02613.1
Cluster 6grp_highest_10_10 > 29      6grp_high1_10_10 > 81      6grp_highest_nonorm > 72      6grp_total > 91      4grp_total > 49      6grp_highest_sub > 04_05      6grp_high1_sub > 04      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 532
1 2 3 4 range
A 69.4
(-3)
A 		474.4
(1.9)
P 		567
(3)
P 		65.7
(-3)
A 		501
B 93.2
(-2.6)
A 		564.7
(3)
P 		508.8
(2.3)
P 		62
(-3)
A 		503
C 86.3
(-3)
A 		593.6
(3)
P 		535.1
(2.3)
P 		125
(-2.5)
A 		507
avr 83
(-3) 		544.2
(3) 		537
(2.9) 		84.2
(-3) 		461

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 389
1 2 3 4 5 6 range
A 486.8
(1)
P 		358.1
(-1.9)
P 		381
(-1.4)
P 		467.3
(0.5)
P 		577
(3)
P 		310
(-3)
P 		267
B 467
(0.9)
P 		397.8
(-0.3)
P 		359.2
(-1)
P 		585.6
(3)
P 		485.4
(1.2)
P 		243.4
(-3)
P 		342
C 327.4
(-3)
P 		369.4
(-2.2)
P 		397.3
(-1.6)
P 		632.1
(3)
P 		440.9
(-0.8)
P 		345.5
(-2.6)
P 		305
avr 427.1
(-0.1) 		375.1
(-1.3) 		379.2
(-1.2) 		561.7
(3) 		501.1
(1.6) 		299.6
(-3) 		262
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 177
1 2 3 4 range
A 25.3
(-2.3)
A 		103
(1)
P 		148.2
(3)
P 		9.6
(-3)
A 		139
B 29.7
(-2.2)
A 		139.5
(3)
P 		117.9
(2)
P 		13.2
(-3)
A 		126
C 28.6
(-2.7)
A 		170.7
(2.4)
P 		186.5
(3)
P 		21.1
(-3)
A 		165
avr 27.9
(-2.4) 		137.7
(2.4) 		150.9
(3) 		14.6
(-3) 		136

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 122
1 2 3 4 5 6 range
A 89.7
(-2.1)
P 		127.3
(0.2)
P 		83
(-2.5)
P 		118.5
(-0.4)
P 		174.1
(3)
P 		75.5
(-3)
P 		99
B 162.5
(1.1)
P 		132.4
(-0.5)
P 		87.2
(-3)
P 		155.1
(0.7)
P 		197.2
(3)
P 		90.7
(-2.8)
P 		110
C 121.3
(-0.1)
P 		112.7
(-1.3)
P 		101.1
(-3)
P 		127.1
(0.8)
P 		142.7
(3)
P 		115.7
(-0.9)
P 		42
avr 124.5
(-0.5) 		124.1
(-0.5) 		90.4
(-3) 		133.6
(0.2) 		171.3
(3) 		94
(-2.7) 		81
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187757_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10675
Sequence Type Exemplar sequence
Representative Public ID F56B3.2 NCBI
Target Description F56B3.2 /REP_DB=WormBase Gene ID /WP=CE17883 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:O45113 protein_id:AAC02613.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067582(10), NM_067583(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067582 NCBI Caenorhabditis elegans F56B3.2a (F56B3.2) mRNA, complete cds. 10/11 None
NM_067583 NCBI Caenorhabditis elegans F56B3.2b (F56B3.2) mRNA, complete cds. 11/11 None
SNAP00000041562 ENSEMBL cdna:SNAP chromosome:CEL140:IV:764178:768359:-1 10/11 None
GENEFINDER00000041573 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:764178:771173:-1 10/11 None
F56B3.2a ENSEMBL cdna:known chromosome:CEL140:IV:764090:768584:-1 gene:F56B3.2 10/11 None
F56B3.2b ENSEMBL cdna:known chromosome:CEL140:IV:764178:768033:-1 gene:F56B3.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:764183-766958(-) 100.0 100.0
Public Domain and Genome References
Ensembl F56B3.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29836 Hypothetical protein F56B3.2a [Caenorhabditis elegans] ref|NP_499983.2| F56B3.2a [Caenorhabditis elegans] 0.0
blast D88633 protein F56B3.2 [imported] - Caenorhabditis elegans 1.0E-168
blast AAK29837 Hypothetical protein F56B3.2b [Caenorhabditis elegans] ref|NP_499984.1| F56B3.2b [Caenorhabditis elegans] 1.0E-132
blast D88633 protein F56B3.2 [imported] - Caenorhabditis elegans 1.0E-132
Protein Domains
Database ID Description E-Value
Pfam IPR006149 EMBL-EBI Nematode-specific EB region 5.7E-7
Pfam IPR006149 EMBL-EBI Nematode-specific EB region 5.7E-7
Pfam IPR006149 EMBL-EBI Nematode-specific EB region 5.7E-7
Pfam IPR006149 EMBL-EBI Nematode-specific EB region 5.7E-7
Sequence
Target Sequence 
>C. ELEGANS:187757_S_AT
gattcccattactgttactttttgtcaggtaatttcaataaaatccaaaattttatttgc
aatgcaaattcagacggaagtttctcccaagccgcttgctgccgtcgtccatgcaatgcg
atggctccaaatgctctctacgtcgactatcactgtatgccacgtgggcaactcaactca
gcgtgcacatcgaatgctcaatgtggtggtggagagggaatggaatgcatgaagggacag
tgtcaatgtcaacaaaatttccacccagcagttgatgtgctcacgaatccattgaaaaat
ccatctcaatcgtgttcgagggactgtgagagtgagaatttgtcaaaggacacaagttgt
atgaaggctgtaccgctcaattccctctgcttcattcaaaagcaatgtccacaaaattcg
ggatgctaccgtggaagatgcatgtgccgttgtgggtttgcagcgaaaaatggaaaatgc
gttgagattccagccccaccaacgacgacttcagcaccagcacccgccataatccccgga
gtgcccatcaatcctggaaacgacctgttcaaacttttcggacaattcctcggagct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCCCATTACTGTTACTTTTTGT 703 423 535 Antisense
TTCAGACGGAAGTTTCTCCCAAGCC 646 687 603 Antisense
GCCGTCGTCCATGCAATGCGATGGC 529 283 635 Antisense
AATGCGATGGCTCCAAATGCTCTCT 292 165 649 Antisense
TACGTCGACTATCACTGTATGCCAC 588 645 673 Antisense
ACTCAACTCAGCGTGCACATCGAAT 558 97 705 Antisense
AAAATTTCCACCCAGCAGTTGATGT 313 133 788 Antisense
GTATGAAGGCTGTACCGCTCAATTC 361 455 893 Antisense
GCATGTGCCGTTGTGGGTTTGCAGC 172 311 974 Antisense
GTGCCCATCAATCCTGGAAACGACC 177 479 1075 Antisense
ACTTTTCGGACAATTCCTCGGAGCT 402 101 1107 Antisense
Sequence Source Affymetrix Proprietary Database