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[187756_at] DIACYLGLYCEROL KINASE

Description T28D9.5 /REP_DB=WormBase Gene ID /WP=CE02070 /CHR=2 /FEA=Sanger Annotation /DEF=(ST.LOUIS) SW:Q10024 protein_id:AAA68308.1
Cluster 6grp_total > 23      4grp_total > 45      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 44
1 2 3 4 range
A 38.4
(3)
A 		32.3
(1.3)
A 		38.3
(3)
A 		16.5
(-3)
A 		22
B 42.9
(3)
A 		32.9
(1.4)
A 		5.9
(-3)
A 		7.7
(-2.7)
A 		37
C 49.8
(3)
A 		46
(2.3)
A 		19.2
(-3)
A 		26.9
(-1.5)
A 		31
avr 43.7
(3) 		37.1
(1.5) 		21.1
(-2.1) 		17
(-3) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 50
1 2 3 4 5 6 range
A 34.1
(1)
A 		42.9
(3)
A 		28.8
(-0.2)
A 		36.7
(1.6)
A 		16.4
(-3)
A 		25.5
(-0.9)
A 		27
B 43.3
(1.3)
A 		24
(-1)
A 		32.7
(-0)
A 		57.8
(3)
A 		7.7
(-3)
A 		12.5
(-2.4)
A 		50
C 52.9
(2.5)
A 		56.5
(3)
A 		48.1
(1.9)
A 		25.9
(-1)
A 		39.7
(0.8)
A 		10.4
(-3)
A 		46
avr 43.4
(3) 		41.1
(2.5) 		36.5
(1.5) 		40.1
(2.3) 		21.3
(-1.9) 		16.1
(-3) 		27
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 14
(3)
A 		7
(-0.6)
A 		10
(0.9)
A 		2.4
(-3)
A 		12
B 13.7
(3)
A 		8.1
(0.3)
A 		1.4
(-3)
A 		1.6
(-2.9)
A 		12
C 16.5
(3)
A 		13.2
(1.4)
A 		6.7
(-1.9)
A 		4.5
(-3)
A 		12
avr 14.7
(3) 		9.4
(0.3) 		6
(-1.4) 		2.8
(-3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 6.3
(-2.2)
A 		15.3
(3)
A 		6.3
(-2.2)
A 		9.3
(-0.5)
A 		5
(-3)
A 		6.2
(-2.3)
A 		10
B 15.1
(2.9)
A 		8
(-0.6)
A 		7.9
(-0.6)
A 		15.3
(3)
A 		3.1
(-3)
A 		4.6
(-2.3)
A 		12
C 19.6
(3)
A 		17.2
(2.1)
A 		12.2
(0.2)
A 		5.2
(-2.4)
A 		12.8
(0.5)
A 		3.5
(-3)
A 		16
avr 13.7
(3) 		13.5
(2.9) 		8.8
(-0.3) 		9.9
(0.4) 		7
(-1.5) 		4.8
(-3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187756_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5063
Sequence Type Exemplar sequence
Representative Public ID T28D9.5 NCBI
Target Description T28D9.5 /REP_DB=WormBase Gene ID /WP=CE02070 /CHR=2 /FEA=Sanger Annotation /DEF=(ST.LOUIS) SW:Q10024 protein_id:AAA68308.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062900(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062900 NCBI Caenorhabditis elegans K06A1.6 (K06A1.6) mRNA, complete cds. 11/11 A 
GENEFINDER00000011388 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6465471:6471032:-1 11/11 None
K06A1.6 ENSEMBL cdna:known chromosome:CEL140:II:6456486:6467589:-1 gene:K06A1.6 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000011373 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6465467-6467465(-) 97.82 97.82
Public Domain and Genome References
Gene Title DIACYLGLYCEROL KINASE
Ensembl K06A1.6
Entrez Gene 174068 Entrez gene
SwissProt Q10024 EMBL-EBI
WormBase CE28048 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.18282.1.S1_S_AT similar to diacylglycerol kinase, zeta 104kDa isoform 2 cfa
CHICKEN:GGA.5646.1.S1_AT diacylglycerol kinase, zeta 104kDa gga
CHICKEN:GGA.5646.2.S1_AT diacylglycerol kinase, zeta 104kDa gga
CHICKEN:GGAAFFX.5280.1.S1_AT diacylglycerol kinase, zeta 104kDa gga
CHICKEN:GGAAFFX.5280.1.S1_S_AT diacylglycerol kinase, zeta 104kDa gga
HG-U133_PLUS_2:207556_S_AT diacylglycerol kinase, zeta 104kDa hs
HG-U133A:207556_S_AT diacylglycerol kinase, zeta 104kDa hs
HG-U133A_2:207556_S_AT diacylglycerol kinase, zeta 104kDa hs
HU35KSUBA:U94905_S_AT diacylglycerol kinase, zeta 104kDa hs
HU35KSUBA:HUM_ALU_AT diacylglycerol kinase, zeta 104kDa hs
HU35KSUBB:HUM_ALU_AT diacylglycerol kinase, zeta 104kDa hs
HU35KSUBC:HUM_ALU_AT diacylglycerol kinase, zeta 104kDa hs
HU35KSUBD:HUM_ALU_AT diacylglycerol kinase, zeta 104kDa hs
HUGENEFL:HUM_ALU_AT diacylglycerol kinase, zeta 104kDa hs
HC-G110:HUM_ALU_AT diacylglycerol kinase, zeta 104kDa hs
HUGENEFL:U51477_AT diacylglycerol kinase, zeta 104kDa hs
U133_X3P:G4503316_3P_A_AT diacylglycerol kinase, zeta 104kDa hs
U133_X3P:HS.165827.0.A1_3P_AT diacylglycerol kinase, zeta 104kDa hs
HG-U133_PLUS_2:239342_AT diacylglycerol kinase, zeta 104kDa hs
HG-U133B:239342_AT diacylglycerol kinase, zeta 104kDa hs
HG-U95AV2:38003_S_AT diacylglycerol kinase, zeta 104kDa hs
HG-U95B:52964_AT diacylglycerol kinase, zeta 104kDa hs
HG-U95D:91775_AT diacylglycerol kinase, zeta 104kDa hs
MU11KSUBB:MSA.18213.0_S_AT diacylglycerol kinase zeta mm
MG-U74BV2:113121_AT diacylglycerol kinase zeta mm
MOE430A:1452169_A_AT diacylglycerol kinase zeta mm
MOUSE430A_2:1452169_A_AT diacylglycerol kinase zeta mm
MOUSE430_2:1452169_A_AT diacylglycerol kinase zeta mm
MOE430A:1426738_AT diacylglycerol kinase zeta mm
MOUSE430A_2:1426738_AT diacylglycerol kinase zeta mm
MOUSE430_2:1426738_AT diacylglycerol kinase zeta mm
MOE430B:1446614_AT Diacylglycerol kinase zeta, mRNA (cDNA clone IMAGE:2650291) mm
MOUSE430_2:1446614_AT Diacylglycerol kinase zeta, mRNA (cDNA clone IMAGE:2650291) mm
MU19KSUBA:TC23469_AT Diacylglycerol kinase zeta, mRNA (cDNA clone IMAGE:2650291) mm
MU19KSUBC:TC38739_AT Diacylglycerol kinase zeta, mRNA (cDNA clone IMAGE:2650291) mm
MU19KSUBC:TC38739_G_AT Diacylglycerol kinase zeta, mRNA (cDNA clone IMAGE:2650291) mm
MU19KSUBC:TC38968_AT Diacylglycerol kinase zeta, mRNA (cDNA clone IMAGE:2650291) mm
RG-U34A:D78588_AT diacylglycerol kinase zeta rn
RAE230A:1367745_AT diacylglycerol kinase zeta rn
RAT230_2:1367745_AT diacylglycerol kinase zeta rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
7205 protein kinase C activation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4143 diacylglycerol kinase activity inferred from electronic annotation QuickGO AmiGO
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
20037 heme binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC24300 Hypothetical protein K06A1.6 [Caenorhabditis elegans] ref|NP_495301.1| K06A1.6 [Caenorhabditis elegans] sp|Q10024|DGKM_CAEEL Putative diacylglycerol kinase K06A1.6 (Diglyceride kinase) (DGK) (DAG kinase) 0.0
blast CAE59319 Hypothetical protein CBG02659 [Caenorhabditis briggsae] 0.0
blast T34334 hypothetical protein K06A1.6 - Caenorhabditis elegans 0.0
blast AAC24300 Hypothetical protein K06A1.6 [Caenorhabditis elegans] ref|NP_495301.1| K06A1.6 [Caenorhabditis elegans] sp|Q10024|DGKM_CAEEL Putative diacylglycerol kinase K06A1.6 (Diglyceride kinase) (DGK) (DAG kinase) 7.0E-67
blast CAE59319 Hypothetical protein CBG02659 [Caenorhabditis briggsae] 4.0E-60
blast CAE59318 Hypothetical protein CBG02658 [Caenorhabditis briggsae] 6.0E-30
Protein Domains
Database ID Description E-Value
Pfam IPR001206 EMBL-EBI Diacylglycerol kinase, catalytic domain 3.4E-62
Pfam IPR000756 EMBL-EBI Diacylglycerol kinase accessory domain (presumed) 4.2E-48
Sequence
Target Sequence 
>C. ELEGANS:187756_AT
tgctcaagaagctgtttctctccgcgaagcagccgaacgtcgatcacaatttgctcgtgt
tccagctcgttcagtttctgctccaccgcgaaaaaagtggtttcgatcgaaacaacaact
atcaggcgccgccgatggaatggacggctcatcgcgtgccggctctcaagagattgtatg
cgacgaattcacgaagaagctcgttgcggcgattaccacgtggcacgacattcactcgtc
tcttctacagttatccatcgagcgctcgtcaaatgattcaaacgaagaacatgagaatgt
atctgtttcatcagaatcatcatggtcatctgcatctgattct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTCAAGAAGCTGTTTCTCTCCGC 122 579 27 Antisense
AAGCAGCCGAACGTCGATCACAATT 647 151 53 Antisense
GATCACAATTTGCTCGTGTTCCAGC 514 421 68 Antisense
ACAACTATCAGGCGCCGCCGATGGA 201 113 141 Antisense
GATGGAATGGACGGCTCATCGCGTG 425 407 160 Antisense
GCGTGCCGGCTCTCAAGAGATTGTA 8 293 180 Antisense
GAAGAAGCTCGTTGCGGCGATTACC 173 339 219 Antisense
TACCACGTGGCACGACATTCACTCG 48 649 240 Antisense
CTTCTACAGTTATCCATCGAGCGCT 1 221 268 Antisense
ATCGAGCGCTCGTCAAATGATTCAA 134 37 283 Antisense
ATCATGGTCATCTGCATCTGATTCT 424 29 345 Antisense
Sequence Source Affymetrix Proprietary Database