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[187754_s_at]

Description F16B4.5 /REP_DB=WormBase Gene ID /WP=CE17015 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:O44631 protein_id:AAB94236.1
Cluster 6grp_high1_10_10 > 77      4grp_highest_8_8 > 23      4grp_high_8_8 > 06      6grp_total > 77      4grp_total > 02      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15081
1 2 3 4 range
A 89.3
(-3)
P 		49.9
(-3)
P 		73.2
(-3)
P 		12074.8
(3)
P 		12025
B 103.3
(-3)
P 		85.5
(-3)
P 		30.5
(-3)
P 		13008.1
(3)
P 		12978
C 114.4
(-3)
P 		72.9
(-3)
P 		31.4
(-3)
P 		15111.2
(3)
P 		15080
avr 102.3
(-3) 		69.4
(-3) 		45
(-3) 		13398
(3) 		13353

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5057
1 2 3 4 5 6 range
A 50.7
(-3)
A 		92.8
(-2.9)
P 		75.9
(-3)
P 		115.7
(-2.9)
P 		982.3
(-1.6)
P 		3947.6
(3)
P 		3897
B 52.4
(-3)
P 		80.9
(-3)
P 		73.5
(-3)
P 		44.2
(-3)
P 		1433.5
(-1.2)
P 		4763.5
(3)
P 		4719
C 49.2
(-3)
P 		96.8
(-2.9)
P 		70.9
(-3)
P 		69.3
(-3)
P 		1115.2
(-1.7)
P 		5101.3
(3)
P 		5052
avr 50.8
(-3) 		90.2
(-2.9) 		73.4
(-3) 		76.4
(-3) 		1177
(-1.5) 		4604.1
(3) 		4553
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2765
1 2 3 4 range
A 32.5
(-2.9)
P 		10.8
(-3)
P 		19.1
(-3)
P 		1767.5
(3)
P 		1757
B 32.9
(-2.9)
P 		21.1
(-3)
P 		7.1
(-3)
P 		2772
(3)
P 		2765
C 37.9
(-2.9)
P 		21
(-3)
P 		10.9
(-3)
P 		2548
(3)
P 		2537
avr 34.4
(-2.9) 		17.6
(-3) 		12.4
(-3) 		2362.5
(3) 		2350

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1767
1 2 3 4 5 6 range
A 9.3
(-3)
A 		33
(-2.9)
P 		16.5
(-3)
P 		29.3
(-2.9)
P 		296.4
(-1.2)
P 		961.1
(3)
P 		952
B 18.2
(-3)
P 		26.9
(-2.9)
P 		17.9
(-3)
P 		11.7
(-3)
P 		582.4
(-1.1)
P 		1776.3
(3)
P 		1765
C 18.2
(-3)
P 		29.5
(-2.9)
P 		18
(-3)
P 		13.9
(-3)
P 		360.9
(-1.8)
P 		1707.7
(3)
P 		1694
avr 15.2
(-3) 		29.8
(-2.9) 		17.5
(-3) 		18.3
(-3) 		413.2
(-1.4) 		1481.7
(3) 		1467
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187754_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15497
Sequence Type Exemplar sequence
Representative Public ID F16B4.5 NCBI
Target Description F16B4.5 /REP_DB=WormBase Gene ID /WP=CE17015 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:O44631 protein_id:AAB94236.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071048(11), NM_071049(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071048 NCBI Caenorhabditis elegans F16B4.5b (F16B4.5) mRNA, complete cds. 11/11 None
NM_071049 NCBI Caenorhabditis elegans F16B4.5a (F16B4.5) mRNA, complete cds. 11/11 None
SNAP00000004017 ENSEMBL cdna:SNAP chromosome:CEL140:V:1591280:1591556:1 11/11 None
GENEFINDER00000004035 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1591280:1591556:1 11/11 None
F16B4.5b ENSEMBL cdna:known chromosome:CEL140:V:1589156:1591586:1 gene:F16B4.5 11/11 None
F16B4.5a ENSEMBL cdna:known chromosome:CEL140:V:1591206:1591556:1 gene:F16B4.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1591279-1591556(+) 100.0 100.0
Public Domain and Genome References
Ensembl F16B4.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68333 Hypothetical protein F16B4.5b [Caenorhabditis elegans] ref|NP_503449.1| F16B4.5b [Caenorhabditis elegans] 3.0E-18
Sequence
Target Sequence 
>C. ELEGANS:187754_S_AT
tgtattggcaattgtcacagcaaaaccgcaccctggatatggacgacctagttacggagg
aggtgactttggtggaggtggtggtcaatggggatcccaatcatcatcgttccagcagaa
tcaaatgtccggtggttttcagaatggcggcggattt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTATTGGCAATTGTCACAGCAAAA 407 555 39 Antisense
CAATTGTCACAGCAAAACCGCACCC 106 179 47 Antisense
GCAAAACCGCACCCTGGATATGGAC 623 317 58 Antisense
GCACCCTGGATATGGACGACCTAGT 510 269 66 Antisense
GGATATGGACGACCTAGTTACGGAG 50 511 73 Antisense
GGACGACCTAGTTACGGAGGAGGTG 650 517 79 Antisense
GGAGGAGGTGACTTTGGTGGAGGTG 145 517 94 Antisense
ATCATCATCGTTCCAGCAGAATCAA 209 31 138 Antisense
GCAGAATCAAATGTCCGGTGGTTTT 259 309 153 Antisense
ATGTCCGGTGGTTTTCAGAATGGCG 172 49 163 Antisense
TGGTTTTCAGAATGGCGGCGGATTT 571 549 171 Antisense
Sequence Source Affymetrix Proprietary Database