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[187748_s_at]

Description C04A2.2 /REP_DB=WormBase Gene ID /WP=CE01769 /CHR=2 /FEA=Sanger Annotation /DEF=(ST.LOUIS) SW:Q09228 protein_id:AAC46733.1
Cluster 6grp_highest_10_10 > 83      6grp_high1_10_10 > 48      4grp_highest_8_8 > 54      4grp_high_8_8 > 10      6grp_highest_nonorm > 74      6grp_total > 37      4grp_total > 22      6grp_highest_sub > Cu      6grp_cgc > 17      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 925
1 2 3 4 range
A 1480.1
(3)
P 		680.3
(-2.3)
P 		582.3
(-3)
P 		1307.4
(1.8)
P 		898
B 1445.5
(2.7)
P 		786.6
(-1.6)
P 		581.6
(-3)
P 		1487.4
(3)
P 		906
C 1338.6
(2)
P 		611.9
(-2.7)
P 		564.9
(-3)
P 		1489.9
(3)
P 		925
avr 1421.4
(3) 		692.9
(-2.2) 		576.3
(-3) 		1428.2
(3) 		852

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 703
1 2 3 4 5 6 range
A 659.4
(0.5)
P 		764.8
(1.6)
P 		317.4
(-3)
P 		614.7
(0)
P 		533.5
(-0.8)
P 		905.3
(3)
P 		588
B 967.8
(3)
P 		682.7
(-1.3)
P 		572.7
(-3)
P 		609.6
(-2.4)
P 		624.4
(-2.2)
P 		877.4
(1.6)
P 		395
C 1019.9
(3)
P 		751.5
(-0.1)
P 		561.2
(-2.3)
P 		678.7
(-0.9)
P 		497.7
(-3)
P 		747.3
(-0.1)
P 		522
avr 882.4
(3) 		733
(0.8) 		483.8
(-3) 		634.3
(-0.7) 		551.9
(-2) 		843.3
(2.4) 		399
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 404
1 2 3 4 range
A 538.9
(3)
P 		147.7
(-3)
P 		152.2
(-2.9)
P 		191.4
(-2.3)
P 		391
B 461
(3)
P 		194.3
(-1.9)
P 		134.8
(-3)
P 		317
(0.4)
P 		326
C 443.1
(3)
P 		175.9
(-3)
P 		196.9
(-2.5)
P 		251.2
(-1.3)
P 		267
avr 481
(3) 		172.6
(-2.8) 		161.3
(-3) 		253.2
(-1.3) 		320

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 309
1 2 3 4 5 6 range
A 121.6
(-1.4)
P 		272
(3)
P 		69.1
(-3)
P 		155.9
(-0.4)
P 		161
(-0.3)
P 		220.4
(1.5)
P 		203
B 336.8
(3)
P 		227.3
(-0.3)
P 		139.1
(-3)
P 		161.5
(-2.3)
P 		253.7
(0.5)
P 		327.2
(2.7)
P 		198
C 378
(3)
P 		229.3
(-0.7)
P 		142.8
(-2.8)
P 		136.5
(-3)
P 		161.1
(-2.4)
P 		250.2
(-0.2)
P 		242
avr 278.8
(3) 		242.9
(1.7) 		117
(-3) 		151.3
(-1.7) 		191.9
(-0.2) 		265.9
(2.5) 		162
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187748_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4281
Sequence Type Exemplar sequence
Representative Public ID C04A2.2 NCBI
Target Description C04A2.2 /REP_DB=WormBase Gene ID /WP=CE01769 /CHR=2 /FEA=Sanger Annotation /DEF=(ST.LOUIS) SW:Q09228 protein_id:AAC46733.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171011(11), NM_001026740(11), NM_001026741(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171011 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-27) (egl-27) mRNA, complete cds. 11/11 A 
NM_001026740 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-27) (egl-27) mRNA, complete cds. 11/11 None
NM_001026741 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-27) (egl-27) mRNA, complete cds. 11/11 None
SNAP00000035664 ENSEMBL cdna:SNAP chromosome:CEL140:II:6845305:6847242:1 11/11 None
GENEFINDER00000035668 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6835402:6847242:1 11/11 None
C04A2.3a ENSEMBL cdna:known chromosome:CEL140:II:6834951:6847836:1 gene:C04A2.3 11/11 A 
C04A2.3b ENSEMBL cdna:known chromosome:CEL140:II:6835011:6847242:1 gene:C04A2.3 11/11 A 
C04A2.3c.1 ENSEMBL cdna:known chromosome:CEL140:II:6842339:6847242:1 gene:C04A2.3 11/11 None
C04A2.3c.2 ENSEMBL cdna:known chromosome:CEL140:II:6845290:6847242:1 gene:C04A2.3 11/11 None
C04A2.3c.3 ENSEMBL cdna:known chromosome:CEL140:II:6845292:6847242:1 gene:C04A2.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6840390-6847518(+) 91.79 100.0
Public Domain and Genome References
Ensembl C04A2.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM81126 Egg laying defective protein 27, isoform a [Caenorhabditis elegans] ref|NP_741012.1| EGg Laying defective family member (egl-27) [Caenorhabditis elegans] sp|Q09228|EGL27_CAEEL Egg laying defective protein 0.0
blast AAD27790 EGL-27 [Caenorhabditis elegans] 0.0
blast AAM81127 Egg laying defective protein 27, isoform b [Caenorhabditis elegans] ref|NP_001021911.1| EGg Laying defective family member (egl-27) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000679 EMBL-EBI Zn-finger, GATA type 1.2E-8
Pfam IPR001005 EMBL-EBI Myb DNA-binding domain 1.6E-9
Pfam IPR000949 EMBL-EBI ELM2 domain 8.5E-18
Pfam IPR000679 EMBL-EBI Zn-finger, GATA type 1.2E-8
Pfam IPR001005 EMBL-EBI Myb DNA-binding domain 1.6E-9
Pfam IPR000679 EMBL-EBI Zn-finger, GATA type 1.2E-8
Pfam IPR001005 EMBL-EBI Myb DNA-binding domain 1.6E-9
Pfam IPR000949 EMBL-EBI ELM2 domain 8.5E-18
Sequence
Target Sequence 
>C. ELEGANS:187748_S_AT
taatggcgaacagcgcagccagccaacaagatattgcacgattaatggagatggctgctc
agcagcaacaacagcaacagcaagctgctcaggctcaagcccagcgtgaccaagaacgtg
aacgacgggaacgtgaagcacgagaacgggaagctgctcgggaacgtgaacgagagcaag
ctgctcgagaagctgcagcacgagatcaggcagcccgcgaacatgctcaagcagttcagg
cagcagcagcagcagcacaacaggcacaggcacttacacctgatatgcaacatatgcatc
tactgcaacagttgatgctaaatccagcacttatgatgcaacttcaacaggctcaagctc
aacaacaacaacaacagcctcaagtaacgaatccccttcaaatgttgcagcatggaatgg
ctgcgcaaagtgccaatcaagccgaaatgatgcgtcggattcatccagaacctgcgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAATGGCGAACAGCGCAGCCAGCCA 276 635 2186 Antisense
CAAGCCCAGCGTGACCAAGAACGTG 50 175 2281 Antisense
GAACGGGAAGCTGCTCGGGAACGTG 101 347 2329 Antisense
CTGCAGCACGAGATCAGGCAGCCCG 145 237 2378 Antisense
GCCCGCGAACATGCTCAAGCAGTTC 657 285 2398 Antisense
AAGCAGTTCAGGCAGCAGCAGCAGC 387 151 2414 Antisense
GCACAACAGGCACAGGCACTTACAC 98 317 2440 Antisense
AACAGCCTCAAGTAACGAATCCCCT 78 137 2558 Antisense
AACGAATCCCCTTCAAATGTTGCAG 488 145 2571 Antisense
GCTGCGCAAAGTGCCAATCAAGCCG 47 297 2605 Antisense
GTCGGATTCATCCAGAACCTGCGAT 173 471 2639 Antisense
Sequence Source Affymetrix Proprietary Database