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[187747_at]

Description B0496.3 /REP_DB=WormBase Gene ID /WP=CE06715 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:Q17521 protein_id:AAB00636.1
Cluster 6grp_highest_10_10 > 42      6grp_high1_10_10 > 23      4grp_highest_8_8 > 63      4grp_high_8_8 > 27      6grp_highest_nonorm > 31      6grp_total > 02      4grp_total > 36      6grp_highest_sub > 02      6grp_high1_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 347
1 2 3 4 range
A 791.8
(1.7)
P 		818.9
(3)
P 		695.1
(-3)
P 		797.1
(1.9)
P 		124
B 816.9
(1.3)
P 		761.7
(-1.7)
P 		738.4
(-3)
P 		848.5
(3)
P 		110
C 850.2
(1.6)
P 		933.1
(3)
P 		585.8
(-3)
P 		726
(-0.6)
P 		347
avr 819.6
(2.3) 		837.9
(3) 		673.1
(-3) 		790.5
(1.3) 		165

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 538
1 2 3 4 5 6 range
A 852.7
(-0.5)
P 		1126.3
(3)
P 		664.8
(-2.9)
P 		794
(-1.3)
P 		815.5
(-1)
P 		660.2
(-3)
P 		466
B 953.3
(3)
P 		928.5
(2.6)
P 		726.9
(-0.7)
P 		847.1
(1.3)
P 		652.8
(-1.9)
P 		588.8
(-3)
P 		365
C 982.5
(1.9)
P 		1064.2
(3)
P 		934.8
(1.3)
P 		852.1
(0.3)
P 		686.4
(-1.9)
P 		597.5
(-3)
P 		467
avr 929.5
(1.4) 		1039.7
(3) 		775.5
(-0.7) 		831.1
(0) 		718.2
(-1.5) 		615.5
(-3) 		424
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 172
1 2 3 4 range
A 288.3
(3)
P 		177.8
(-0.9)
P 		181.7
(-0.7)
P 		116.7
(-3)
P 		172
B 260.5
(3)
P 		188.1
(-1.9)
P 		171.1
(-3)
P 		180.8
(-2.3)
P 		89
C 281.4
(3)
P 		268.3
(2.5)
P 		204.2
(0.1)
P 		122.4
(-3)
P 		159
avr 276.7
(3) 		211.4
(0.1) 		185.7
(-1) 		140
(-3) 		137

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 256
1 2 3 4 5 6 range
A 157.2
(-2.7)
P 		400.6
(3)
P 		144.8
(-3)
P 		201.4
(-1.7)
P 		246.1
(-0.6)
P 		160.7
(-2.6)
P 		256
B 331.7
(3)
P 		309.1
(2.1)
P 		176.5
(-3)
P 		224.4
(-1.1)
P 		265.2
(0.4)
P 		219.5
(-1.3)
P 		155
C 364.2
(3)
P 		324.7
(1.8)
P 		237.9
(-0.9)
P 		171.4
(-3)
P 		222.1
(-1.4)
P 		200
(-2.1)
P 		193
avr 284.4
(0.7) 		344.8
(3) 		186.4
(-3) 		199.1
(-2.5) 		244.5
(-0.8) 		193.4
(-2.7) 		158
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187747_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9017
Sequence Type Exemplar sequence
Representative Public ID B0496.3 NCBI
Target Description B0496.3 /REP_DB=WormBase Gene ID /WP=CE06715 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:Q17521 protein_id:AAB00636.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068785(9), NM_068784(9), NM_182282(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068785 NCBI Caenorhabditis elegans B0496.3b (B0496.3) mRNA, complete cds. 9/11 None
NM_068784 NCBI Caenorhabditis elegans B0496.3a (B0496.3) mRNA, complete cds. 9/11 None
NM_182282 NCBI Caenorhabditis elegans B0496.3c (B0496.3) mRNA, complete cds. 9/11 None
SNAP00000020644 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7435567:7444344:1 9/11 None
GENEFINDER00000020653 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7440773:7444344:1 11/11 None
B0496.3a ENSEMBL cdna:known chromosome:CEL140:IV:7431332:7444344:1 gene:B0496.3 9/11 None
B0496.3b ENSEMBL cdna:known chromosome:CEL140:IV:7431332:7444632:1 gene:B0496.3 9/11 A 
B0496.3c ENSEMBL cdna:known chromosome:CEL140:IV:7440673:7444344:1 gene:B0496.3 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7431337-7444350(+) 98.91 100.0
Public Domain and Genome References
Ensembl B0496.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAU05598 Hypothetical protein B0496.3b [Caenorhabditis elegans] ref|NP_501186.3| B0496.3b [Caenorhabditis elegans] 0.0
blast AAU05597 Hypothetical protein B0496.3a [Caenorhabditis elegans] ref|NP_501185.3| B0496.3a [Caenorhabditis elegans] 0.0
blast T29253 hypothetical protein B0496.3 - Caenorhabditis elegans 0.0
blast NP_872082 B0496.3c [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 5.0E-77
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 4.0E-77
hanks 1.9.5 AGC group; AGC Other; DdK2 4.0E-92
hanks 1.9.5 AGC group; AGC Other; DdK2 3.0E-92
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.5E-92
Sequence
Target Sequence 
>C. ELEGANS:187747_AT
gagtgctgacatcgaaccacgtgttgaatcgtcttatgagccaagcaatggagttcgatc
gcatgaatttcttgatcaacttctaagcatgcgtttcattctccaggtgttcgaacgtct
tcgtcgtcatttgtctctcgagaaaagtgtttcgccgcaaagacaggttgcatctgtcgg
accacatagtcgaaatattgatagcacatcatctgcattagacaatccaaaaaagaaaag
atcattgctctcattcaatcggagaaagacaagtgaagtacgaatgggtgccgatggaaa
gctggttacaaatggatatgatagtacaccgagtagcagagatttcaagagaccaagctc
cccaatcgatcggataaagtctctgttcagaaagaatgaaagttcaggaactgggcactc
ggattattataattcttcaagaaactacacatcttcaaatccggtttccacaactcgcga
gccatatgttgcccaatatagaaaatatccaggttcaacctcacgtgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGTGCTGACATCGAACCACGTGTT 400 401 4074 Antisense
TCTAAGCATGCGTTTCATTCTCCAG 564 613 4155 Antisense
TCTCCAGGTGTTCGAACGTCTTCGT 521 609 4173 Antisense
TTCGTCGTCATTTGTCTCTCGAGAA 322 695 4193 Antisense
AAGTGTTTCGCCGCAAAGACAGGTT 408 157 4218 Antisense
GTTGCATCTGTCGGACCACATAGTC 408 439 4240 Antisense
CAAGCTCCCCAATCGATCGGATAAA 441 185 4427 Antisense
GTTCAGGAACTGGGCACTCGGATTA 323 445 4475 Antisense
ACTACACATCTTCAAATCCGGTTTC 605 99 4517 Antisense
CAACTCGCGAGCCATATGTTGCCCA 82 185 4544 Antisense
ATATCCAGGTTCAACCTCACGTGAT 459 19 4578 Antisense
Sequence Source Affymetrix Proprietary Database