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[187744_s_at]

Description C15E6.1 /REP_DB=WormBase Gene ID /WP=CE16848 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:O45055 protein_id:AAC02559.1
Cluster 6grp_total > 34      4grp_total > 61      6grp_cgc > 21      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 94
1 2 3 4 range
A 117.9
(3)
A 		108.1
(0.5)
A 		109.3
(0.8)
A 		94.6
(-3)
A 		23
B 109.7
(3)
A 		98.8
(2.2)
A 		73.3
(0.5)
A 		24
(-3)
A 		86
C 114.7
(3)
A 		101.5
(1.8)
A 		103.5
(2)
A 		50.4
(-3)
A 		64
avr 114.1
(3) 		102.8
(1.8) 		95.4
(1.1) 		56.3
(-3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 121
1 2 3 4 5 6 range
A 75.4
(2.1)
A 		83.5
(3)
A 		68.9
(1.5)
A 		59.2
(0.4)
A 		26.6
(-3)
A 		41.3
(-1.4)
A 		57
B 67.8
(3)
A 		55.4
(1.8)
A 		52.8
(1.6)
A 		4.6
(-3)
A 		30.6
(-0.5)
A 		54.8
(1.8)
A 		63
C 125.1
(3)
A 		62
(-0.6)
A 		95.6
(1.3)
A 		19.9
(-3)
A 		65.2
(-0.4)
A 		35.4
(-2.1)
A 		105
avr 89.4
(3) 		67
(0.8) 		72.4
(1.3) 		27.9
(-3) 		40.8
(-1.7) 		43.8
(-1.4) 		62
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 42.9
(3)
A 		23.5
(-1)
A 		28.6
(0.1)
A 		13.8
(-3)
A 		29
B 35
(3)
A 		24.4
(0.9)
A 		17
(-0.6)
A 		5.1
(-3)
A 		30
C 38
(3)
A 		29.2
(1.2)
A 		36.1
(2.6)
A 		8.5
(-3)
A 		30
avr 38.6
(3) 		25.7
(0.4) 		27.2
(0.7) 		9.1
(-3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 45
1 2 3 4 5 6 range
A 13.9
(-1.4)
A 		29.7
(3)
A 		15
(-1.1)
A 		15
(-1.1)
A 		8
(-3)
A 		10
(-2.4)
A 		22
B 23.6
(3)
A 		18.4
(1.6)
A 		12.8
(0.1)
A 		1.2
(-3)
A 		12.5
(0)
A 		20.4
(2.1)
A 		22
C 46.4
(3)
A 		18.9
(-0.9)
A 		24.3
(-0.1)
A 		4
(-3)
A 		21.1
(-0.6)
A 		11.9
(-1.9)
A 		42
avr 28
(3) 		22.3
(1.4) 		17.4
(0) 		6.7
(-3) 		13.9
(-1) 		14.1
(-0.9) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187744_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10451
Sequence Type Exemplar sequence
Representative Public ID C15E6.1 NCBI
Target Description C15E6.1 /REP_DB=WormBase Gene ID /WP=CE16848 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:O45055 protein_id:AAC02559.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067449(11), NM_067455(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067449 NCBI Caenorhabditis elegans Y38C1AB.4 (Y38C1AB.4) mRNA, complete cds. 11/11 A 
NM_067455 NCBI Caenorhabditis elegans FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-5) (frm-5) mRNA, complete cds. 11/11 A 
SNAP00000022363 ENSEMBL cdna:SNAP chromosome:CEL140:IV:45826:48844:-1 9/11 None
SNAP00000032132 ENSEMBL cdna:SNAP chromosome:CEL140:IV:644:9937:1 11/11 None
GENEFINDER00000032150 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:695:8548:1 11/11 None
Y38C1AB.4 ENSEMBL cdna:known chromosome:CEL140:IV:695:14926:1 gene:Y38C1AB.4 11/11 A 
Y38C1AB.8 ENSEMBL cdna:known chromosome:CEL140:IV:32953:47184:-1 gene:Y38C1AB.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:694-2323(+) 100.0 100.0
chrIV:45555-47184(-) 100.0 100.0
Public Domain and Genome References
Gene Title

Gene Symbol frm-5
Ensembl Y38C1AB.4
Y38C1AB.8
Entrez Gene 176821 Entrez gene
176822 Entrez gene
SwissProt Q9N531 EMBL-EBI
Q9N530 EMBL-EBI
WormBase CE25254 Wormbase
CE27782 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5856 cytoskeleton inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5515 protein binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF60499 Hypothetical protein Y38C1AB.4 [Caenorhabditis elegans] ref|NP_499850.1| Y38C1AB.4 [Caenorhabditis elegans] 0.0
blast CAE58494 Hypothetical protein CBG01639 [Caenorhabditis briggsae] 0.0
blast AAF60494 Ferm domain (protein4.1-ezrin-radixin-moesin) family protein 5 [Caenorhabditis elegans] ref|NP_499856.1| FERM domain (protein4.1-ezrin-radixin-moesin) family member (frm-5) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000299 EMBL-EBI Band 4.1 3.7E-7
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 3.1E-9
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.3E-8
Pfam IPR000299 EMBL-EBI Band 4.1 3.7E-7
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 3.1E-9
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.3E-8
Pfam IPR000299 EMBL-EBI Band 4.1 3.7E-7
Pfam IPR000299 EMBL-EBI Band 4.1 3.7E-7
Sequence
Target Sequence 
>C. ELEGANS:187744_S_AT
gaagcccaaaccgaaggctatcccggcttttgataacgcctatgacgccgactttgacaa
ttccccaccacttcatcattactcggcggttcatttggaaactgggctctcaccgcttga
ggaggctcagagagctctccgagccaaccgagcaaggcataagccgtcgaatgtatcgtt
ggcagaggaggcgaagcttgctgccagacagagatactcgaatgcatcggatattagaag
agaagaggaggaagaagtggtggaagaagatccagcagtggtagttccagttctacggaa
ggatctggaagttgaggaggctccgaaatcggtacgaccgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGCCCAAACCGAAGGCTATCCCG 618 283 750 Antisense
CTATCCCGGCTTTTGATAACGCCTA 264 225 767 Antisense
TATGACGCCGACTTTGACAATTCCC 383 657 790 Antisense
CACCACTTCATCATTACTCGGCGGT 162 201 815 Antisense
TCATTTGGAAACTGGGCTCTCACCG 295 619 840 Antisense
CTCTCACCGCTTGAGGAGGCTCAGA 507 229 856 Antisense
GAGGCTCAGAGAGCTCTCCGAGCCA 425 405 871 Antisense
TCTCCGAGCCAACCGAGCAAGGCAT 508 609 885 Antisense
GGCGAAGCTTGCTGCCAGACAGAGA 67 543 939 Antisense
GATCCAGCAGTGGTAGTTCCAGTTC 384 415 1018 Antisense
GAGGCTCCGAAATCGGTACGACCGC 219 403 1066 Antisense
Sequence Source Affymetrix Proprietary Database