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[187732_at]

Description H20J04.5 /REP_DB=WormBase Gene ID /WP=CE23815 /TR=SW:Q9N5M2 /GB=AAF39891.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 23      6grp_high1_10_10 > 30      4grp_highest_8_8 > 01      4grp_high_8_8 > 45      6grp_highest_nonorm > 57      6grp_total > 43      4grp_total > 50      6grp_highest_sub > 01_02_03      6grp_cgc > 71      6grp_high1_sub > 03      6grp_cgc_sub > 03      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4164
1 2 3 4 range
A 2399
(-2.5)
P 		4665.9
(3)
P 		4328.8
(2.2)
P 		2186
(-3)
P 		2480
B 2583.5
(-1.5)
P 		5147.8
(3)
P 		3890.2
(0.8)
P 		1708.9
(-3)
P 		3439
C 2886.7
(-0.8)
P 		5556.6
(3)
P 		3942.5
(0.7)
P 		1393.1
(-3)
P 		4164
avr 2623.1
(-1.5) 		5123.4
(3) 		4053.8
(1.1) 		1762.7
(-3) 		3361

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1674
1 2 3 4 5 6 range
A 1786.4
(-2.3)
P 		3253
(3)
P 		2395.9
(-0.1)
P 		2772.3
(1.3)
P 		1579.1
(-3)
P 		2281.2
(-0.5)
P 		1674
B 2439.6
(0.9)
P 		2156.6
(-0.8)
P 		2778
(3)
P 		1969
(-2)
P 		1815.8
(-2.9)
P 		1800.5
(-3)
P 		978
C 2915.7
(3)
P 		1968.9
(-2.9)
P 		2783.7
(2.2)
P 		1958.7
(-3)
P 		2313.8
(-0.8)
P 		2035.3
(-2.5)
P 		957
avr 2380.6
(0.8) 		2459.5
(1.5) 		2652.5
(3) 		2233.3
(-0.4) 		1902.9
(-3) 		2039
(-1.9) 		750
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1363
1 2 3 4 range
A 873.5
(1.1)
P 		1012.8
(2.1)
P 		1131.6
(3)
P 		320
(-3)
P 		812
B 823.8
(0)
P 		1271.4
(3)
P 		901.4
(0.6)
P 		364.2
(-3)
P 		907
C 955.6
(0.2)
P 		1597.6
(3)
P 		1374.1
(2)
P 		234.9
(-3)
P 		1363
avr 884.3
(0.5) 		1293.9
(3) 		1135.7
(2) 		306.4
(-3) 		988

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 828
1 2 3 4 5 6 range
A 329.3
(-3)
P 		1156.9
(3)
P 		521.8
(-1.6)
P 		703.1
(-0.3)
P 		476.5
(-1.9)
P 		555.4
(-1.4)
P 		828
B 848.9
(3)
P 		717.8
(0.6)
P 		674.6
(-0.2)
P 		521.5
(-3)
P 		737.7
(1)
P 		671.4
(-0.3)
P 		327
C 1080.7
(3)
P 		600.8
(-1.2)
P 		708.5
(-0.3)
P 		393.9
(-3)
P 		748.7
(0.1)
P 		681.3
(-0.5)
P 		687
avr 753
(1.5) 		825.2
(3) 		635
(-1) 		539.5
(-3) 		654.3
(-0.6) 		636
(-1) 		286
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187732_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5831
Sequence Type Exemplar sequence
Representative Public ID H20J04.5 NCBI
Target Description H20J04.5 /REP_DB=WormBase Gene ID /WP=CE23815 /TR=SW:Q9N5M2 /GB=AAF39891.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062363(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062363 NCBI Caenorhabditis elegans H20J04.5 (H20J04.5) mRNA, complete cds. 11/11 None
SNAP00000017487 ENSEMBL cdna:SNAP chromosome:CEL140:II:4326713:4328138:1 11/11 None
GENEFINDER00000017495 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4326713:4328138:1 10/11 None
H20J04.5.1 ENSEMBL cdna:known chromosome:CEL140:II:4326711:4328271:1 gene:H20J04.5 11/11 None
H20J04.5.2 ENSEMBL cdna:known chromosome:CEL140:II:4326713:4328351:1 gene:H20J04.5 11/11 None
H20J04.5.3 ENSEMBL cdna:known chromosome:CEL140:II:4326713:4328351:1 gene:H20J04.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4326711-4328137(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol tag-355
Ensembl H20J04.5
Entrez Gene 173768 Entrez gene
SwissProt Q9N5M2 EMBL-EBI
WormBase CE23815 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF39891 Temporarily assigned gene name protein 355 [Caenorhabditis elegans] ref|NP_494764.1| H20J04.5 [Caenorhabditis elegans] sp|Q9N5M2|PFD2_CAEEL Probable prefoldin subunit 3.0E-63
blast AAF39891 Temporarily assigned gene name protein 355 [Caenorhabditis elegans] ref|NP_494764.1| H20J04.5 [Caenorhabditis elegans] sp|Q9N5M2|PFD2_CAEEL Probable prefoldin subunit 1.0E-59
blast CAE60125 Hypothetical protein CBG03668 [Caenorhabditis briggsae] 6.0E-52
Protein Domains
Database ID Description E-Value
Pfam IPR002777 EMBL-EBI Prefoldin-related KE2 protein 2.3E-6
Sequence
Target Sequence 
>C. ELEGANS:187732_AT
gccactccagccgagcaagaagagcaacgaaaagtcgtcgaaaagttcaaggctctacgt
gatcaacaacaggatatcgccgccgaagtgactcgaatcgaagaggaacgacgtgaattt
ggacgtgttctcgaagtgatcaaggatcttgaaccggatcagaaatgcttccgtttgatt
agtgatacacttgtcgagtatactgtcaaggatgttattccggatttgcagaataatatc
gctaatttgacaatcgtctcgaagcagctcaacgatcagctcgtcgaaaagggaaaagag
ctgaacactcacaaaactacacacaacattcgtctgctcactgagaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCACTCCAGCCGAGCAAGAAGAGC 434 279 37 Antisense
AGTTCAAGGCTCTACGTGATCAACA 710 63 80 Antisense
TCAACAACAGGATATCGCCGCCGAA 697 629 99 Antisense
GCCGCCGAAGTGACTCGAATCGAAG 710 283 115 Antisense
TTGGACGTGTTCTCGAAGTGATCAA 392 707 155 Antisense
GAAATGCTTCCGTTTGATTAGTGAT 621 361 198 Antisense
ATCGCTAATTTGACAATCGTCTCGA 112 37 274 Antisense
GAAGCAGCTCAACGATCAGCTCGTC 469 341 297 Antisense
GAACACTCACAAAACTACACACAAC 675 351 339 Antisense
ACTACACACAACATTCGTCTGCTCA 423 101 352 Antisense
CAACATTCGTCTGCTCACTGAGAAA 303 189 360 Antisense
Sequence Source Affymetrix Proprietary Database