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[187731_at]

Description ZC410.7 /REP_DB=WormBase Gene ID /WP=CE18435 /CHR=4 /FEA=Sanger Annotation /DEF=(HINXTON) TR:O62503 protein_id:CAA92571.1
Cluster 6grp_highest_10_10 > 73      6grp_high1_10_10 > 37      4grp_highest_8_8 > 34      4grp_high_8_8 > 57      6grp_highest_nonorm > 73      6grp_total > 45      4grp_total > 62      6grp_highest_sub > Cu      6grp_cgc > 06      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 233
1 2 3 4 range
A 765.6
(-1.8)
P 		859.9
(3)
P 		812.5
(0.6)
P 		741
(-3)
P 		119
B 974.3
(3)
P 		823.1
(-2.6)
P 		961.8
(2.5)
P 		813.1
(-3)
P 		161
C 919.7
(3)
P 		812.8
(-3)
P 		888.9
(1.3)
P 		821.4
(-2.5)
P 		107
avr 886.5
(2.9) 		831.9
(-0.5) 		887.7
(3) 		791.8
(-3) 		96

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 343
1 2 3 4 5 6 range
A 561.7
(-0.8)
P 		711.4
(3)
P 		571.5
(-0.6)
P 		476.3
(-3)
P 		496.3
(-2.5)
P 		632.6
(1)
P 		235
B 530.4
(1.5)
P 		447.7
(-3)
P 		557.4
(3)
P 		450.3
(-2.9)
P 		542
(2.2)
P 		537.6
(1.9)
P 		110
C 790.2
(3)
P 		560.8
(-1.4)
P 		577.8
(-1)
P 		474.3
(-3)
P 		546.8
(-1.6)
P 		599.6
(-0.6)
P 		316
avr 627.4
(3) 		573.3
(1) 		568.9
(0.8) 		467
(-3) 		528.4
(-0.7) 		589.9
(1.6) 		160
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 202
1 2 3 4 range
A 278.8
(3)
P 		186.7
(-0.2)
P 		212.4
(0.7)
P 		108.5
(-3)
P 		170
B 310.7
(3)
P 		203.3
(-1.7)
P 		222.9
(-0.8)
P 		173.3
(-3)
P 		137
C 304.4
(2.8)
P 		233.7
(0.3)
P 		309.8
(3)
P 		138.5
(-3)
P 		171
avr 298
(3) 		207.9
(-0.4) 		248.4
(1.1) 		140.1
(-3) 		158

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 198
1 2 3 4 5 6 range
A 103.6
(-3)
P 		253
(3)
P 		124.5
(-2.2)
P 		120.8
(-2.3)
P 		149.8
(-1.1)
P 		154
(-1)
P 		149
B 184.6
(0.9)
P 		149
(-1.2)
P 		135.4
(-2)
P 		119.3
(-3)
P 		220.2
(3)
P 		200.5
(1.8)
P 		101
C 292.9
(3)
P 		171.1
(-0.7)
P 		147.1
(-1.4)
P 		95.4
(-3)
P 		176.9
(-0.5)
P 		200.7
(0.2)
P 		198
avr 193.7
(3) 		191
(2.8) 		135.7
(-1.2) 		111.8
(-3) 		182.3
(2.2) 		185.1
(2.4) 		82
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187731_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8598
Sequence Type Exemplar sequence
Representative Public ID ZC410.7 NCBI
Target Description ZC410.7 /REP_DB=WormBase Gene ID /WP=CE18435 /CHR=4 /FEA=Sanger Annotation /DEF=(HINXTON) TR:O62503 protein_id:CAA92571.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028430(11), NM_001028429(11), NM_069179(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028430 NCBI Caenorhabditis elegans LiPoate Ligase family member (lpl-1) (lpl-1) mRNA, complete cds. 11/11 None
NM_001028429 NCBI Caenorhabditis elegans LiPoate Ligase family member (lpl-1) (lpl-1) mRNA, complete cds. 11/11 None
NM_069179 NCBI Caenorhabditis elegans LiPoate Ligase family member (lpl-1) (lpl-1) mRNA, complete cds. 11/11 A 
SNAP00000030929 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9093955:9094424:-1 11/11 None
GENEFINDER00000030935 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9092439:9094424:-1 10/11 None
ZC410.7c ENSEMBL cdna:known chromosome:CEL140:IV:9092195:9094424:-1 gene:ZC410.7 11/11 A 
ZC410.7a.1 ENSEMBL cdna:known chromosome:CEL140:IV:9092261:9094436:-1 gene:ZC410.7 11/11 None
ZC410.7b ENSEMBL cdna:known chromosome:CEL140:IV:9092439:9094424:-1 gene:ZC410.7 11/11 None
ZC410.7a.2 ENSEMBL cdna:known chromosome:CEL140:IV:9093955:9094448:-1 gene:ZC410.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9093960-9094484(-) 100.0 100.0
Public Domain and Genome References
Ensembl ZC410.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAC35869 Hypothetical protein ZC410.7b [Caenorhabditis elegans] ref|NP_001023600.1| LiPoate Ligase family member (lpl-1) [Caenorhabditis elegans] 1.0E-171
blast CAE54911 Hypothetical protein ZC410.7c [Caenorhabditis elegans] ref|NP_001023601.1| LiPoate Ligase family member (lpl-1) [Caenorhabditis elegans] 1.0E-170
blast CAC35869 Hypothetical protein ZC410.7b [Caenorhabditis elegans] ref|NP_001023600.1| LiPoate Ligase family member (lpl-1) [Caenorhabditis elegans] 1.0E-169
blast CAE54911 Hypothetical protein ZC410.7c [Caenorhabditis elegans] ref|NP_001023601.1| LiPoate Ligase family member (lpl-1) [Caenorhabditis elegans] 1.0E-169
blast CAC35869 Hypothetical protein ZC410.7b [Caenorhabditis elegans] ref|NP_001023600.1| LiPoate Ligase family member (lpl-1) [Caenorhabditis elegans] 1.0E-148
blast CAE54911 Hypothetical protein ZC410.7c [Caenorhabditis elegans] ref|NP_001023601.1| LiPoate Ligase family member (lpl-1) [Caenorhabditis elegans] 1.0E-146
Protein Domains
Database ID Description E-Value
Pfam IPR004143 EMBL-EBI Biotin/lipoate A/B protein ligase domain 1.1E-17
Pfam IPR004143 EMBL-EBI Biotin/lipoate A/B protein ligase domain 2.7E-17
Pfam IPR004143 EMBL-EBI Biotin/lipoate A/B protein ligase domain 9.7E-25
Sequence
Target Sequence 
>C. ELEGANS:187731_AT
atcacttatcgattcgcttcatctgattctccgaggaaagtcgtagtttgtgcaaacgga
acgattgctgcctggcatccaccacaacattttccatacgagcatacgaaaccaattgat
ctcggttcactgactaaaaaggatcaaagctctcgtttgtcagcagcagcaaaagcttct
tccattccacgcgagccagttaacgctgagctcaaggacattttctacacgagcaagcac
gagtggtattccagaactcgcgaaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCACTTATCGATTCGCTTCATCTG 673 27 88 Antisense
TTCGCTTCATCTGATTCTCCGAGGA 344 697 100 Antisense
AAACGGAACGATTGCTGCCTGGCAT 4 127 141 Antisense
ACCACAACATTTTCCATACGAGCAT 327 85 168 Antisense
ACCAATTGATCTCGGTTCACTGACT 302 85 198 Antisense
AAAGCTCTCGTTTGTCAGCAGCAGC 623 123 233 Antisense
AGCAGCAAAAGCTTCTTCCATTCCA 385 75 252 Antisense
CACGCGAGCCAGTTAACGCTGAGCT 236 199 275 Antisense
ACGCTGAGCTCAAGGACATTTTCTA 604 91 290 Antisense
GGACATTTTCTACACGAGCAAGCAC 486 525 303 Antisense
AGTGGTATTCCAGAACTCGCGAAGA 166 59 329 Antisense
Sequence Source Affymetrix Proprietary Database