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[187730_at]

Description F23F12.3 /REP_DB=WormBase Gene ID /WP=CE01250 /TR=SW:P46499 /GB=AAA20605.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 82      4grp_total > 61      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 96
1 2 3 4 range
A 87.3
(1.8)
P 		73.3
(0.8)
A 		104.9
(3)
A 		19.4
(-3)
A 		86
B 114.9
(3)
P 		71.5
(-1)
A 		56.2
(-2.4)
A 		49.2
(-3)
A 		66
C 81.4
(1.7)
P 		75.1
(1.1)
M 		94.8
(3)
P 		33.6
(-3)
A 		61
avr 94.5
(3) 		73.3
(0.9) 		85.3
(2.1) 		34.1
(-3) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 31.5
(0.2)
A 		19.8
(-2)
A 		44.4
(2.6)
A 		15.8
(-2.7)
A 		46.8
(3)
A 		14.2
(-3)
A 		33
B 18.8
(-1)
A 		39.6
(1.8)
A 		25
(-0.2)
A 		27.5
(0.2)
A 		48.5
(3)
A 		4
(-3)
A 		45
C 9.7
(-3)
A 		49.6
(3)
A 		37.2
(1.1)
A 		42.9
(2)
A 		16.6
(-2)
A 		14.9
(-2.2)
A 		40
avr 20
(-0.9) 		36.3
(2.8) 		35.5
(2.6) 		28.7
(1) 		37.3
(3) 		11
(-3) 		26
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 31.8
(3)
P 		15.9
(-0.3)
A 		27.4
(2.1)
A 		2.8
(-3)
A 		29
B 36.7
(3)
P 		17.7
(-1.4)
A 		13
(-2.4)
A 		10.5
(-3)
A 		26
C 26.9
(1.7)
P 		21.6
(0.5)
M 		33
(3)
P 		5.7
(-3)
A 		27
avr 31.8
(3) 		18.4
(-0.2) 		24.5
(1.3) 		6.3
(-3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 5.8
(-1.7)
A 		7
(-1)
A 		9.7
(0.5)
A 		4
(-2.7)
A 		14.1
(3)
A 		3.5
(-3)
A 		11
B 6.5
(-1.4)
A 		13.2
(0.9)
A 		6.1
(-1.5)
A 		7.3
(-1.1)
A 		19.7
(3)
A 		1.5
(-3)
A 		18
C 3.6
(-3)
A 		15.1
(3)
A 		9.5
(0.1)
A 		8.6
(-0.4)
A 		5.4
(-2.1)
A 		5
(-2.3)
A 		12
avr 5.3
(-1.8) 		11.8
(2.2) 		8.4
(0.1) 		6.6
(-1) 		13.1
(3) 		3.3
(-3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187730_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6979
Sequence Type Exemplar sequence
Representative Public ID F23F12.3 NCBI
Target Description F23F12.3 /REP_DB=WormBase Gene ID /WP=CE01250 /TR=SW:P46499 /GB=AAA20605.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066032(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066032 NCBI Caenorhabditis elegans F23F12.3 (F23F12.3) mRNA, complete cds. 11/11 None
GENEFINDER00000039911 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6500540:6504628:1 11/11 None
F23F12.3 ENSEMBL cdna:known chromosome:CEL140:III:6500394:6507652:1 gene:F23F12.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000039918 1/11 Cross Hyb Matching Probes None
SNAP00000039906 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6500538-6501067(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F23F12.3
Entrez Gene 175926 Entrez gene
SwissProt P46499 EMBL-EBI
WormBase CE37224 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5215 transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_498433 F23F12.3 [Caenorhabditis elegans] gb|AAA20605.3| Hypothetical protein F23F12.3 [Caenorhabditis elegans] sp|P46499|YLX3_CAEEL Hypothetical protein F23F12.3 in chromosome III 0.0
blast AAY86205 Hypothetical protein F23F12.13 [Caenorhabditis elegans] 0.0
blast CAE73649 Hypothetical protein CBG21150 [Caenorhabditis briggsae] 1.0E-144
blast NP_498433 F23F12.3 [Caenorhabditis elegans] gb|AAA20605.3| Hypothetical protein F23F12.3 [Caenorhabditis elegans] sp|P46499|YLX3_CAEEL Hypothetical protein F23F12.3 in chromosome III 2.0E-73
blast AAY86205 Hypothetical protein F23F12.13 [Caenorhabditis elegans] 3.0E-59
blast CAE73648 Hypothetical protein CBG21149 [Caenorhabditis briggsae] 1.0E-58
Protein Domains
Database ID Description E-Value
Pfam IPR005828 EMBL-EBI General substrate transporter 1.5E-5
Pfam IPR005828 EMBL-EBI General substrate transporter 9.5E-9
Pfam IPR005828 EMBL-EBI General substrate transporter 7.6E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498433.2 12 13-35,96-118,125-147,151-173,200-222,226-243,298-320,340-362,369-389,404-426,438-460,465-487
F23F12.3 12 13-35,96-118,125-147,151-173,200-222,226-243,298-320,340-362,369-389,404-426,438-460,465-487
GENEFINDER00000039911 3 13-35,96-118,125-144
Sequence
Target Sequence 
>C. ELEGANS:187730_AT
ttaatcgctttcatattttgtgtttttttttatggcaattcggacttttttacgcttgcc
agttgatttttccgattttctacaattttcatccagggctttcatgcgaagatccaggat
ttaacttttcaaagcctaaatgcaaactttcaaaagtggaaatatgcacggagcttacag
caaattgttccaagtggcacattgagccagctcctttccactcaatggttcaggatttta
aaatgttttgtggaacaaaagcttatgattctgcatggattgcaacaattcaattcattg
gagcacttgttggagcattggtttatggtcatttaggagaccattttggtagaaaaccag
tttcgtttgttggaatctcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTAATCGCTTTCATATTTTGTGTTT 304 685 29 Antisense
GGCAATTCGGACTTTTTTACGCTTG 398 533 62 Antisense
TACGCTTGCCAGTTGATTTTTCCGA 503 649 79 Antisense
TTCTACAATTTTCATCCAGGGCTTT 585 683 106 Antisense
CCAGGGCTTTCATGCGAAGATCCAG 153 269 121 Antisense
GATCCAGGATTTAACTTTTCAAAGC 273 413 139 Antisense
GGAGCTTACAGCAAATTGTTCCAAG 115 521 198 Antisense
AAGTGGCACATTGAGCCAGCTCCTT 464 159 220 Antisense
CTCCTTTCCACTCAATGGTTCAGGA 518 233 239 Antisense
GGAGCACTTGTTGGAGCATTGGTTT 188 521 328 Antisense
ACCAGTTTCGTTTGTTGGAATCTCG 630 85 384 Antisense
Sequence Source Affymetrix Proprietary Database