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[187719_at]

Description Y74C9A.5 /REP_DB=WormBase Gene ID /WP=CE24663 /TR=SW:Q9N4D6 /GB=AAF36051.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 10      6grp_high1_10_10 > 23      4grp_highest_8_8 > 51      4grp_high_8_8 > 32      6grp_highest_nonorm > 71      6grp_total > 03      4grp_total > 47      6grp_high1_sub > 02      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 215
1 2 3 4 range
A 374.2
(3)
P 		301.4
(-1.5)
P 		276.3
(-3)
P 		288.9
(-2.2)
P 		98
B 370.5
(3)
P 		230.7
(-2.7)
P 		223.4
(-3)
P 		241.2
(-2.3)
P 		147
C 410.6
(3)
P 		195.2
(-3)
P 		219.9
(-2.3)
P 		202
(-2.8)
P 		215
avr 385.1
(3) 		242.4
(-2.9) 		239.9
(-3) 		244
(-2.8) 		145

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 299
1 2 3 4 5 6 range
A 388.3
(0.5)
P 		495.2
(3)
P 		235.6
(-3)
P 		489.3
(2.9)
P 		326.1
(-0.9)
P 		295
(-1.6)
P 		260
B 365.8
(3)
P 		301.8
(0.7)
P 		302.7
(0.8)
P 		331.8
(1.8)
P 		350.6
(2.5)
P 		196.6
(-3)
P 		169
C 461.3
(2.2)
P 		436.7
(1.6)
P 		494.3
(3)
P 		380.1
(0.3)
P 		362.5
(-0.1)
P 		239
(-3)
P 		255
avr 405.1
(2.8) 		411.2
(3) 		344.2
(0.6) 		400.4
(2.6) 		346.4
(0.7) 		243.5
(-3) 		168
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 136.2
(3)
P 		65.4
(-1.5)
P 		72.2
(-1.1)
P 		42.3
(-3)
P 		94
B 118.2
(3)
P 		57
(-2.5)
P 		51.8
(-3)
P 		51.4
(-3)
P 		67
C 135.9
(3)
P 		56.1
(-1.7)
P 		76.7
(-0.5)
P 		34.1
(-3)
P 		102
avr 130.1
(3) 		59.5
(-1.8) 		66.9
(-1.3) 		42.6
(-3) 		88

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 125
1 2 3 4 5 6 range
A 71.6
(-2)
P 		176.1
(3)
P 		51.3
(-3)
P 		124.1
(0.5)
P 		98.4
(-0.7)
P 		71.8
(-2)
P 		125
B 127.3
(1.7)
P 		100.5
(-0.6)
P 		73.5
(-3)
P 		87.9
(-1.7)
P 		142.5
(3)
P 		73.3
(-3)
P 		69
C 171
(3)
P 		133.2
(0.6)
P 		125.8
(0.1)
P 		76.4
(-3)
P 		117.3
(-0.4)
P 		80
(-2.8)
P 		95
avr 123.3
(1.7) 		136.6
(3) 		83.5
(-2.2) 		96.1
(-0.9) 		119.4
(1.3) 		75
(-3) 		62
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187719_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2585
Sequence Type Exemplar sequence
Representative Public ID Y74C9A.5 NCBI
Target Description Y74C9A.5 /REP_DB=WormBase Gene ID /WP=CE24663 /TR=SW:Q9N4D6 /GB=AAF36051.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058263(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058263 NCBI Caenorhabditis elegans Y74C9A.5 (Y74C9A.5) mRNA, complete cds. 11/11 None
SNAP00000028624 ENSEMBL cdna:SNAP chromosome:CEL140:I:28280:32452:-1 11/11 None
GENEFINDER00000028630 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:28280:32790:-1 11/11 None
Y74C9A.5 ENSEMBL cdna:known chromosome:CEL140:I:27595:34905:-1 gene:Y74C9A.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:28279-34905(-) 97.67 97.67
Public Domain and Genome References
Gene Title
Ensembl Y74C9A.5
Entrez Gene 171593 Entrez gene
SwissProt Q9N4D6 EMBL-EBI
WormBase CE24663 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.7099.1.S1_AT similar to Sestrin-3 cfa
DROSOPHILA_2:1635678_AT dm
DROSGENOME1:154871_AT dm
CHICKEN:GGAAFFX.11036.1.S1_AT similar to Sestrin 3 gga
HG-U133_PLUS_2:235683_AT sestrin 3 hs
HG-U133B:235683_AT sestrin 3 hs
HU35KSUBC:RC_W95428_AT sestrin 3 hs
U133_X3P:HS2.350558.1.S1_3P_AT sestrin 3 hs
U133_X3P:HS.132927.0.S1_3P_AT sestrin 3 hs
HG-U133_PLUS_2:1553869_AT sestrin 3 hs
HG-U133_PLUS_2:235684_S_AT sestrin 3 hs
HG-U133B:235684_S_AT sestrin 3 hs
HG-U95B:47489_AT sestrin 3 hs
HG-U133_PLUS_2:242899_AT Sestrin 3 hs
HG-U133B:242899_AT Sestrin 3 hs
HU35KSUBA:RC_AA147719_AT Sestrin 3 hs
HU35KSUBB:RC_N62640_AT Sestrin 3 hs
U133_X3P:HS.34636.0.A1_3P_AT Sestrin 3 hs
U133_X3P:HS.271953.0.A1_3P_AT Sestrin 3 hs
U133_X3P:HS.196734.0.A1_3P_AT Sestrin 3 hs
U133_X3P:HS.159441.0.A1_3P_AT Sestrin 3 hs
HG-U133_PLUS_2:225123_AT Sestrin 3 hs
HG-U133B:225123_AT Sestrin 3 hs
HG-U133_PLUS_2:235150_AT Sestrin 3 hs
HG-U133B:235150_AT Sestrin 3 hs
HG-U133_PLUS_2:242906_AT Sestrin 3 hs
HG-U133B:242906_AT Sestrin 3 hs
HG-U95B:44709_AT Sestrin 3 hs
HG-U95B:53406_AT Sestrin 3 hs
HG-U95E:69302_AT Sestrin 3 hs
HG-U133_PLUS_2:241015_AT Sestrin 3 hs
HG-U133B:241015_AT Sestrin 3 hs
HG-U133_PLUS_2:243546_AT Sestrin 3 hs
HG-U133B:243546_AT Sestrin 3 hs
HG-U95B:45056_AT Sestrin 3 hs
HG-U95C:66394_AT Sestrin 3 hs
HG-U95E:87332_AT Sestrin 3 hs
HU35KSUBC:RC_H89127_AT Sestrin 3 hs
HU35KSUBC:RC_N64473_AT Sestrin 3 hs
U133_X3P:HS.132927.1.A1_3P_AT Sestrin 3 hs
U133_X3P:HS.271552.0.S1_3P_AT Sestrin 3 hs
MG-U74BV2:115940_AT sestrin 3 mm
MOE430A:1449303_AT sestrin 3 mm
MOUSE430A_2:1449303_AT sestrin 3 mm
MOUSE430_2:1449303_AT sestrin 3 mm
MOE430B:1453313_AT sestrin 3 mm
MOUSE430_2:1453313_AT sestrin 3 mm
MG-U74CV2:136292_AT Sestrin 3 (Sesn3), mRNA mm
MOE430B:1441705_AT Sestrin 3 (Sesn3), mRNA mm
MOUSE430_2:1441705_AT Sestrin 3 (Sesn3), mRNA mm
RAE230B:1393620_AT sestrin 3 (predicted) rn
RAT230_2:1393620_AT sestrin 3 (predicted) rn
RAE230B:1390910_AT Sestrin 3 (predicted) rn
RAT230_2:1390910_AT Sestrin 3 (predicted) rn
RG-U34C:RC_AI230386_AT Sestrin 3 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7050 cell cycle arrest inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF36051 Hypothetical protein Y74C9A.5 [Caenorhabditis elegans] ref|NP_490664.1| Y74C9A.5 [Caenorhabditis elegans] 0.0
blast Q9N4D6 Putative sestrin 0.0
blast CAE60323 Hypothetical protein CBG03915 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006730 EMBL-EBI PA26 p53-induced protein (sestrin) 5.1E-14
Pfam IPR006730 EMBL-EBI PA26 p53-induced protein (sestrin) 1.0E-126
Pfam IPR006730 EMBL-EBI PA26 p53-induced protein (sestrin) 4.6E-15
Pfam IPR006730 EMBL-EBI PA26 p53-induced protein (sestrin) 1.0E-126
Pfam IPR006730 EMBL-EBI PA26 p53-induced protein (sestrin) 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_490664.1 1 82-104
Y74C9A.5 1 82-104
Sequence
Target Sequence 
>C. ELEGANS:187719_AT
gatacactgacgtcacggcttgatcgaatgttcgatcatattcggactcttactggcaat
atgccatccggctcatcccatgcatctggagacggagaaccaccagccgccgtgaatcag
aatgaaattgacactgctgcattccgtgaagcaatttggaactatactcaaggattatat
ggtgttcgagtcgatgattatgattattcgaaaattaatcgagtacttgacaaaggaact
aaaactttcattaaactggcagcttgctatccacataaattgacaactgaattcacgaga
gctctgccaggatttaaggattcggaaaagcagaaaggaaaaaattccaaaaaaaactcg
gaaaaaatcgaaaaaaaattccgaaaaaaacacagnnnnnnnnnnnnnnnnnnnnnnnnn
nnnnnnnnnnnnnnnnnncatatccacgtggtaatgatggtagcgatggctcgtttccag
gcttcaatgttccattatacacgtgctgtg

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATACACTGACGTCACGGCTTGATC 416 423 1420 Antisense
GATCATATTCGGACTCTTACTGGCA 634 419 1453 Antisense
ACTGGCAATATGCCATCCGGCTCAT 78 97 1471 Antisense
CTCATCCCATGCATCTGGAGACGGA 578 229 1491 Antisense
CTGGAGACGGAGAACCACCAGCCGC 334 241 1505 Antisense
CACCAGCCGCCGTGAATCAGAATGA 429 201 1520 Antisense
TTGACACTGCTGCATTCCGTGAAGC 393 703 1547 Antisense
AAACTGGCAGCTTGCTATCCACATA 611 125 1672 Antisense
GAATTCACGAGAGCTCTGCCAGGAT 340 325 1708 Antisense
TGATGGTAGCGATGGCTCGTTTCCA 219 567 1874 Antisense
AATGTTCCATTATACACGTGCTGTG 392 163 1905 Antisense
Sequence Source Affymetrix Proprietary Database