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[187713_at]

Description C27D8.5 /REP_DB=WormBase Gene ID /WP=CE08424 /CHR=4 /FEA=Sanger Annotation /DEF=(HINXTON) TR:Q93292 protein_id:CAB02265.1
Cluster 6grp_high1_10_10 > 08      6grp_total > 91      4grp_total > 38      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 257
1 2 3 4 range
A 324.5
(3)
P 		150.6
(-3)
P 		167
(-2.4)
M 		185.7
(-1.8)
A 		174
B 350.5
(3)
P 		176.2
(-1.1)
M 		93.1
(-3)
P 		156.3
(-1.5)
A 		257
C 269.8
(3)
P 		179.9
(-0.7)
P 		124.7
(-2.9)
P 		123.1
(-3)
A 		147
avr 314.9
(3) 		168.9
(-1.7) 		128.3
(-3) 		155
(-2.1) 		187

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 159
1 2 3 4 5 6 range
A 191.7
(1.1)
M 		167.2
(-1)
P 		144.2
(-3)
P 		203.6
(2.1)
P 		213.8
(3)
P 		206.1
(2.3)
P 		70
B 264.2
(3)
P 		197.9
(-0)
P 		132.4
(-3)
A 		223.8
(1.2)
P 		233.1
(1.6)
P 		143.2
(-2.5)
P 		132
C 133.7
(-1.8)
A 		186.2
(0.5)
P 		105.6
(-3)
A 		242.5
(3)
A 		181.7
(0.3)
P 		128.3
(-2)
P 		137
avr 196.5
(1.3) 		183.8
(0.5) 		127.4
(-3) 		223.3
(3) 		209.5
(2.1) 		159.2
(-1) 		96
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 118.1
(3)
P 		32.7
(-2.6)
P 		43.7
(-1.9)
M 		27.2
(-3)
A 		91
B 111.8
(3)
P 		43.5
(-1.5)
M 		21.6
(-3)
P 		33.3
(-2.2)
A 		90
C 89.3
(3)
P 		51.7
(-0.3)
P 		43.5
(-1)
P 		20.8
(-3)
A 		69
avr 106.4
(3) 		42.6
(-1.8) 		36.3
(-2.3) 		27.1
(-3) 		79

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 35.3
(-2.3)
M 		59.5
(2.1)
P 		31.4
(-3)
P 		51.6
(0.7)
P 		64.5
(3)
P 		50.2
(0.4)
P 		33
B 91.9
(2.7)
P 		65.9
(0.2)
P 		32.2
(-3)
A 		59.3
(-0.4)
P 		94.7
(3)
P 		53.4
(-1)
P 		63
C 49.5
(1.3)
A 		56.8
(2.6)
P 		26.9
(-3)
A 		48.8
(1.1)
A 		58.8
(3)
P 		43
(0)
P 		32
avr 58.9
(1.1) 		60.7
(1.3) 		30.2
(-3) 		53.2
(0.2) 		72.7
(3) 		48.9
(-0.4) 		43
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187713_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10174
Sequence Type Exemplar sequence
Representative Public ID C27D8.5 NCBI
Target Description C27D8.5 /REP_DB=WormBase Gene ID /WP=CE08424 /CHR=4 /FEA=Sanger Annotation /DEF=(HINXTON) TR:Q93292 protein_id:CAB02265.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028226(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028226 NCBI Caenorhabditis elegans T23B5.3a (T23B5.3) mRNA, complete cds. 11/11 None
SNAP00000003309 ENSEMBL cdna:SNAP chromosome:CEL140:IV:12701784:12703633:1 11/11 None
GENEFINDER00000003317 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:12701123:12703633:1 11/11 None
T23B5.3a ENSEMBL cdna:known chromosome:CEL140:IV:12691919:12703970:1 gene:T23B5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
178222 2 1 NM_001028226 187713_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12702247-12703639(+) 100.0 100.0
Public Domain and Genome References
Ensembl T23B5.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAC42261 Hypothetical protein T23B5.3a [Caenorhabditis elegans] emb|CAB07675.4| Hypothetical protein T23B5.3a [Caenorhabditis elegans] ref|NP_001023397.1| T23B5.3a [Caenorhabditis elegans] 0.0
blast CAE46674 Hypothetical protein T23B5.3b [Caenorhabditis elegans] ref|NP_001023398.1| T23B5.3b [Caenorhabditis elegans] 0.0
blast CAE59995 Hypothetical protein CBG03488 [Caenorhabditis briggsae] 0.0
blast CAC42261 Hypothetical protein T23B5.3a [Caenorhabditis elegans] emb|CAB07675.4| Hypothetical protein T23B5.3a [Caenorhabditis elegans] ref|NP_001023397.1| T23B5.3a [Caenorhabditis elegans] 1.0E-137
blast CAB02265 Hypothetical protein T23B5.3c [Caenorhabditis elegans] 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:187713_AT
cactcccgtcgtctactgaaaacgtacatcctgcatcgaattcaccgccaatgaaacaaa
ttgaaggatttgagtttaagacaccaacacgagcacgcagcgcactcagttcaccgatga
gcaatttcatgaacggcggagacaacgattcaccgatttctcgtccggcttcacgtttga
tgcaacgtctggaacagaagggcacaattcgcagagatgatgaaattatttcacaagaga
ttcgccgcgtcgcagagacgaatctctcacgatcaccgtctctggcgtcacttcgtggtt
caatgtcgaatttggcttttcgtgcgagtcaactgaatctggctggtacttctggagaat
tgccgccaaatctcactaaattcgcgagcgtgaactcaccagcacgtcaatcactgtcat
caatgaaaaagactacggatcgtcaagctgcagttcttgaacgtcttggacgtctccgcg
aatcacttcatgccggcaagaactacacaccgccacgcaacggaatctcatcatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACTCCCGTCGTCTACTGAAAACGT 153 97 170 Antisense
AACGTACATCCTGCATCGAATTCAC 575 143 190 Antisense
ACAACGATTCACCGATTTCTCGTCC 527 113 311 Antisense
AGACGAATCTCTCACGATCACCGTC 486 71 425 Antisense
TGGCGTCACTTCGTGGTTCAATGTC 55 545 452 Antisense
GAATTGCCGCCAAATCTCACTAAAT 29 325 526 Antisense
CACCAGCACGTCAATCACTGTCATC 126 203 566 Antisense
TACGGATCGTCAAGCTGCAGTTCTT 248 647 603 Antisense
TTGAACGTCTTGGACGTCTCCGCGA 230 703 626 Antisense
TCCGCGAATCACTTCATGCCGGCAA 77 595 644 Antisense
GCCACGCAACGGAATCTCATCATCA 562 279 681 Antisense
Sequence Source Affymetrix Proprietary Database