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[187709_at]

Description F30B5.2 /REP_DB=WormBase Gene ID /WP=CE04463 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:Q19915 protein_id:AAA83498.1
Cluster 6grp_highest_10_10 > 20      6grp_high1_10_10 > 01      4grp_highest_8_8 > 31      4grp_high_8_8 > 12      6grp_highest_nonorm > 90      6grp_total > 04      4grp_total > 11      6grp_highest_sub > De      6grp_high1_sub > De      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 107
1 2 3 4 range
A 127.7
(-0.5)
P 		87.8
(-3)
P 		90.4
(-2.8)
P 		184
(3)
P 		96
B 76.7
(-3)
P 		119.5
(0.5)
P 		97.2
(-1.3)
P 		149.3
(3)
P 		73
C 143.5
(1.1)
P 		134.1
(0.2)
P 		96.3
(-3)
P 		166.2
(3)
P 		70
avr 116
(-1.2) 		113.8
(-1.4) 		94.6
(-3) 		166.5
(3) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 178
1 2 3 4 5 6 range
A 114.6
(0.2)
P 		147.5
(3)
P 		114.3
(0.2)
P 		108.4
(-0.3)
P 		93.2
(-1.5)
P 		75.8
(-3)
P 		72
B 157.6
(3)
P 		129.1
(1.2)
P 		129.1
(1.2)
P 		118.8
(0.6)
P 		91.7
(-1)
P 		59.9
(-3)
P 		98
C 223.9
(3)
P 		121.1
(-0.5)
P 		103.2
(-1.1)
P 		125.7
(-0.3)
P 		109.4
(-0.9)
P 		45.8
(-3)
P 		178
avr 165.4
(3) 		132.6
(1.1) 		115.5
(0.1) 		117.6
(0.3) 		98.1
(-0.8) 		60.5
(-3) 		105
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 46.5
(3)
P 		19.1
(-3)
P 		23.6
(-2)
P 		26.9
(-1.3)
P 		27
B 24.5
(-1.7)
P 		29.5
(1.5)
P 		22.5
(-3)
P 		31.8
(3)
P 		9
C 47.5
(3)
P 		38.5
(0.2)
P 		33.6
(-1.3)
P 		28
(-3)
P 		20
avr 39.5
(3) 		29
(-1.9) 		26.6
(-3) 		28.9
(-1.9) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 21.1
(-2.5)
P 		52.4
(3)
P 		24.9
(-1.9)
P 		27.5
(-1.4)
P 		28.1
(-1.3)
P 		18.5
(-3)
P 		34
B 54.8
(3)
P 		43
(0.8)
P 		31.4
(-1.3)
P 		31.5
(-1.3)
P 		37.2
(-0.2)
P 		22.3
(-3)
P 		33
C 83
(3)
P 		37
(-1.1)
P 		26.3
(-2)
P 		25.3
(-2.1)
P 		35.4
(-1.2)
P 		15.3
(-3)
P 		68
avr 53
(3) 		44.1
(1.4) 		27.5
(-1.5) 		28.1
(-1.4) 		33.6
(-0.4) 		18.7
(-3) 		34
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187709_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10840
Sequence Type Exemplar sequence
Representative Public ID F30B5.2 NCBI
Target Description F30B5.2 /REP_DB=WormBase Gene ID /WP=CE04463 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS) TR:Q19915 protein_id:AAA83498.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068116(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068116 NCBI Caenorhabditis elegans F30B5.7 (F30B5.7) mRNA, complete cds. 10/11 A 
SNAP00000023157 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4230082:4231838:-1 10/11 None
GENEFINDER00000023161 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4226052:4231838:-1 10/11 None
F30B5.7 ENSEMBL cdna:known chromosome:CEL140:IV:4229188:4231853:-1 gene:F30B5.7 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4231584-4231859(-) 98.68 98.68
Public Domain and Genome References
Gene Title
Ensembl F30B5.7
Entrez Gene 177186 Entrez gene
SwissProt Q19913 EMBL-EBI
WormBase CE28225 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
30001 metal ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
46873 metal ion transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_500517 F30B5.7 [Caenorhabditis elegans] gb|AAA83498.2| Hypothetical protein F30B5.7 [Caenorhabditis elegans] 1.0E-170
blast NP_500517 F30B5.7 [Caenorhabditis elegans] gb|AAA83498.2| Hypothetical protein F30B5.7 [Caenorhabditis elegans] 1.0E-135
blast NP_500517 F30B5.7 [Caenorhabditis elegans] gb|AAA83498.2| Hypothetical protein F30B5.7 [Caenorhabditis elegans] 1.0E-120
blast CAE61457 Hypothetical protein CBG05348 [Caenorhabditis briggsae] 1.0E-111
blast NP_500516 Serpentine Receptor, class BC (class B-like) family member (srbc-70) [Caenorhabditis elegans] gb|AAA83495.1| Hypothetical protein F30B5.6 [Caenorhabditis elegans] 1.0E-110
blast NP_001023162 Serpentine Receptor, class BC (class B-like) family member (srbc-71) [Caenorhabditis elegans] gb|AAW88401.1| Hypothetical protein F30B5.8 [Caenorhabditis elegans] 1.0E-85
blast CAE70853 Hypothetical protein CBG17638 [Caenorhabditis briggsae] 4.0E-82
Protein Domains
Database ID Description E-Value
Pfam IPR003689 EMBL-EBI Zinc transporter ZIP 1.8E-52
Pfam IPR003689 EMBL-EBI Zinc transporter ZIP 4.0E-35
Pfam IPR003689 EMBL-EBI Zinc transporter ZIP 1.3E-9
Pfam IPR002892 EMBL-EBI Protein of unknown function DUF40 4.1E-86
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500517.1 7 10-30,43-65,85-102,202-224,234-256,268-290,303-320
F30B5.7 7 10-30,43-65,85-102,202-224,234-256,268-290,303-320
SNAP00000023157 3 10-30,43-65,85-102
GENEFINDER00000023161 11 10-30,43-65,85-102,288-310,330-352,365-382,420-442,463-485,518-537,550-572,587-609
Sequence
Target Sequence 
>C. ELEGANS:187709_AT
ggagacactccatacaatggtaacagctacagtactacagtacatccttgcatttgcaat
gtttgtagtaacagctgcaattggaattgttcccctttttattgtgcgggtgatgaaacg
aagtcagaacgaaagtgagcaggggtacctgtcctatttgacttgctttgccgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGACACTCCATACAATGGTAACA 580 517 15 Antisense
ATGGTAACAGCTACAGTACTACAGT 363 51 31 Antisense
GTACTACAGTACATCCTTGCATTTG 707 459 46 Antisense
ACATCCTTGCATTTGCAATGTTTGT 150 107 56 Antisense
GTTTGTAGTAACAGCTGCAATTGGA 331 451 75 Antisense
TGCAATTGGAATTGTTCCCCTTTTT 4 577 90 Antisense
TCCCCTTTTTATTGTGCGGGTGATG 252 475 105 Antisense
GTGCGGGTGATGAAACGAAGTCAGA 369 477 118 Antisense
AAGTGAGCAGGGGTACCTGTCCTAT 267 159 147 Antisense
GGTACCTGTCCTATTTGACTTGCTT 266 505 158 Antisense
TGTCCTATTTGACTTGCTTTGCCGG 458 557 164 Antisense
Sequence Source Affymetrix Proprietary Database