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[187704_at]

Description ZK1127.5 /REP_DB=WormBase Gene ID /WP=CE05125 /TR=SW:Q23400 /GB=AAB93426.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 69      6grp_high1_10_10 > 97      4grp_highest_8_8 > 21      4grp_high_8_8 > 15      6grp_highest_nonorm > 97      6grp_total > 86      4grp_total > 08      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 953
1 2 3 4 range
A 1094.6
(-3)
P 		1330.8
(-0.7)
P 		1337.8
(-0.6)
P 		1709
(3)
P 		614
B 1248.6
(-1.8)
P 		1078.6
(-3)
P 		1318.4
(-1.4)
P 		1958.1
(3)
P 		880
C 1005.5
(-3)
P 		1085.6
(-2.5)
P 		1342.8
(-0.8)
P 		1929.1
(3)
P 		924
avr 1116.2
(-3) 		1165
(-2.6) 		1333
(-1.3) 		1865.4
(3) 		749

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 726
1 2 3 4 5 6 range
A 944.3
(-1.1)
P 		839.5
(-2.5)
P 		798.7
(-3)
P 		1121.1
(1.2)
P 		1062
(0.5)
P 		1256.2
(3)
P 		458
B 788.1
(-3)
P 		1039
(-0.1)
P 		961.2
(-1)
P 		1015.2
(-0.4)
P 		1165.6
(1.4)
P 		1306.3
(3)
P 		518
C 694.3
(-3)
P 		1004
(-0.4)
P 		903.2
(-1.3)
P 		1040.8
(-0.1)
P 		1006.2
(-0.4)
P 		1420
(3)
P 		726
avr 808.9
(-3) 		960.8
(-1.2) 		887.7
(-2.1) 		1059
(-0.1) 		1077.9
(0.1) 		1327.5
(3) 		519
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 218
1 2 3 4 range
A 398.5
(3)
P 		288.9
(-1.4)
P 		349.7
(1)
P 		250.2
(-3)
P 		148
B 398.2
(2.2)
P 		266.4
(-3)
P 		305.5
(-1.4)
P 		417.3
(3)
P 		151
C 332.9
(-2.2)
P 		312.1
(-3)
P 		468
(3)
P 		325.3
(-2.5)
P 		156
avr 376.5
(3) 		289.1
(-3) 		374.4
(2.9) 		330.9
(-0.1) 		87

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 313
1 2 3 4 5 6 range
A 174.1
(-3)
P 		298.6
(2.1)
P 		173.9
(-3)
P 		284.3
(1.5)
P 		320.5
(3)
P 		305.8
(2.4)
P 		147
B 274.2
(-2)
P 		345.8
(-0.3)
P 		233.4
(-3)
P 		268.9
(-2.2)
P 		473.6
(2.7)
P 		487.1
(3)
P 		254
C 257.3
(-1.9)
P 		306.3
(-0.8)
P 		229.9
(-2.5)
P 		209.3
(-3)
P 		325.6
(-0.4)
P 		475.4
(3)
P 		266
avr 235.2
(-2.3) 		316.9
(-0) 		212.4
(-3) 		254.2
(-1.8) 		373.2
(1.6) 		422.8
(3) 		210
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187704_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5213
Sequence Type Exemplar sequence
Representative Public ID ZK1127.5 NCBI
Target Description ZK1127.5 /REP_DB=WormBase Gene ID /WP=CE05125 /TR=SW:Q23400 /GB=AAB93426.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063044(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063044 NCBI Caenorhabditis elegans ZK1127.5 (ZK1127.5) mRNA, complete cds. 11/11 None
SNAP00000033589 ENSEMBL cdna:SNAP chromosome:CEL140:II:7050605:7051838:1 11/11 None
GENEFINDER00000033607 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7050605:7051838:1 11/11 None
ZK1127.5 ENSEMBL cdna:known chromosome:CEL140:II:7050602:7051919:1 gene:ZK1127.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7050875-7051834(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK1127.5
Entrez Gene 174151 Entrez gene
SwissProt Q23400 EMBL-EBI
WormBase CE32083 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1584891_AT similar to RNA cyclase homolog cfa
CANINE_2:CFA.5493.1.A1_AT similar to RNA cyclase homolog cfa
CANINE_2:CFAAFFX.4051.1.S1_S_AT similar to RNA cyclase homolog cfa
DROSOPHILA_2:1638146_AT dm
DROSGENOME1:141535_AT dm
CHICKEN:GGA.9558.1.S1_AT RNA terminal phosphate cyclase-like 1 gga
HG-U133B:222666_S_AT RNA terminal phosphate cyclase-like 1 hs
HG-U133_PLUS_2:222666_S_AT RNA terminal phosphate cyclase-like 1 hs
HG-U133_PLUS_2:218544_S_AT RNA terminal phosphate cyclase-like 1 hs
HG-U133A:218544_S_AT RNA terminal phosphate cyclase-like 1 hs
HG-FOCUS:218544_S_AT RNA terminal phosphate cyclase-like 1 hs
HG-U133A_2:218544_S_AT RNA terminal phosphate cyclase-like 1 hs
HU35KSUBB:RC_N51773_AT RNA terminal phosphate cyclase-like 1 hs
HU35KSUBD:RC_AA382401_F_AT RNA terminal phosphate cyclase-like 1 hs
HU35KSUBD:RC_R05309_F_AT RNA terminal phosphate cyclase-like 1 hs
U133_X3P:G5032042_3P_X_AT RNA terminal phosphate cyclase-like 1 hs
U133_X3P:G5032042_3P_A_AT RNA terminal phosphate cyclase-like 1 hs
U133_X3P:G12654400_3P_A_AT RNA terminal phosphate cyclase-like 1 hs
HG-U95B:48502_AT RNA terminal phosphate cyclase-like 1 hs
HG-U95E:91398_AT RNA terminal phosphate cyclase-like 1 hs
HG-U133_PLUS_2:233981_AT RNA terminal phosphate cyclase-like 1 hs
HG-U133B:233981_AT RNA terminal phosphate cyclase-like 1 hs
HU35KSUBC:RC_N52822_AT RNA terminal phosphate cyclase-like 1 hs
U133_X3P:HS2.375778.1.S1_3P_AT RNA terminal phosphate cyclase-like 1 hs
U133_X3P:HS.113052.1.S1_3P_X_AT RNA terminal phosphate cyclase-like 1 hs
HG-U133_PLUS_2:1558489_AT RNA terminal phosphate cyclase-like 1 hs
HG-U133_PLUS_2:233729_AT RNA terminal phosphate cyclase-like 1 hs
HG-U133B:233729_AT RNA terminal phosphate cyclase-like 1 hs
HG-U95D:68117_R_AT RNA terminal phosphate cyclase-like 1 hs
MU11KSUBA:AA177990_S_AT RNA terminal phosphate cyclase-like 1 mm
MG-U74AV2:98923_AT RNA terminal phosphate cyclase-like 1 mm
MOE430A:1416730_AT RNA terminal phosphate cyclase-like 1 mm
MOUSE430A_2:1416730_AT RNA terminal phosphate cyclase-like 1 mm
MOUSE430_2:1416730_AT RNA terminal phosphate cyclase-like 1 mm
MU19KSUBA:TC23059_I_AT RNA terminal phosphate cyclase-like 1, mRNA (cDNA clone MGC:6408 IMAGE:3586632) mm
MU19KSUBC:TC38879_AT RNA terminal phosphate cyclase-like 1, mRNA (cDNA clone MGC:6408 IMAGE:3586632) mm
YEAST_2:1773154_AT RNA terminal phosphate cyclase-like protein involved in rRNA processing at sites A0, A1, and A2; does not possess detectable RNA cyclase activity Sc
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3963 RNA-3'-phosphate cyclase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB93426 Hypothetical protein ZK1127.5 [Caenorhabditis elegans] ref|NP_495445.2| ZK1127.5 [Caenorhabditis elegans] sp|Q23400|RCL1_CAEEL Probable RNA 3'-terminal phosphate cyclase-like protein 0.0
blast CAE66018 Hypothetical protein CBG11211 [Caenorhabditis briggsae] 0.0
blast G88196 protein ZK1127.5 [imported] - Caenorhabditis elegans 1.0E-171
Protein Domains
Database ID Description E-Value
Pfam IPR000228 EMBL-EBI RNA 3-terminal phosphate cyclase 1.9E-93
Pfam IPR000228 EMBL-EBI RNA 3-terminal phosphate cyclase 2.8E-48
Sequence
Target Sequence 
>C. ELEGANS:187704_AT
gttacaccttcactagcttatcgaatgattgacgctgcaaagaaagcaatgcatgggtac
atttcggatgtttatattactgttgatcaacggaaaggtgatgctggtggcgcttctcca
ggatatggtctgttcttaaccgccgaaacgacagagggtgttatctaccaagcagaagct
atttcaagaccgaagggcgaatcaggagttccaattcttccagaagacattggaattgaa
gccggtcatgctcttcttcaacaaatttacatgggaggtgcccttgacagtagtgctcaa
atacttgcaagcacatttatgacactctgtcctaaagatgtttcacacttcctctacggt
cctcttccaatgtatagtgtacacgcactcaggcatctcaaacagttttttgagatcgag
tttaaaatggaagactttaaaaaaacattgaaggaagaggaagctgatctcaaaacagga
agtgccgataaagctatggttaccgctgttggtgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTACACCTTCACTAGCTTATCGAA 95 445 376 Antisense
GCGCTTCTCCAGGATATGGTCTGTT 212 287 485 Antisense
TATGGTCTGTTCTTAACCGCCGAAA 362 655 499 Antisense
GAAGGGCGAATCAGGAGTTCCAATT 544 333 567 Antisense
AATTGAAGCCGGTCATGCTCTTCTT 188 179 609 Antisense
ATGGGAGGTGCCCTTGACAGTAGTG 108 49 646 Antisense
CAAGCACATTTATGACACTCTGTCC 429 185 683 Antisense
TAAAGATGTTTCACACTTCCTCTAC 124 641 708 Antisense
ACGGTCCTCTTCCAATGTATAGTGT 62 95 731 Antisense
TAGTGTACACGCACTCAGGCATCTC 639 653 750 Antisense
AAGCTATGGTTACCGCTGTTGGTGT 207 151 866 Antisense
Sequence Source Affymetrix Proprietary Database