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[187701_at]

Description T25D10.3 /REP_DB=WormBase Gene ID /WP=CE02060 /TR=SW:Q10018 /GB=AAC46590.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 77      4grp_total > 02      6grp_cgc > 49      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5869
1 2 3 4 range
A 36.6
(-3)
A 		59.2
(-3)
A 		49.9
(-3)
A 		5894.3
(3)
P 		5858
B 69.6
(-3)
A 		63.5
(-3)
A 		25.1
(-3)
A 		5720.5
(3)
P 		5695
C 84.5
(-3)
A 		70.4
(-3)
A 		42.3
(-3)
A 		5147.9
(3)
P 		5106
avr 63.6
(-3) 		64.4
(-3) 		39.1
(-3) 		5587.6
(3) 		5549

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3810
1 2 3 4 5 6 range
A 9.2
(-3)
A 		48
(-2.9)
A 		60.9
(-2.9)
A 		67.5
(-2.9)
P 		861.4
(-1.7)
P 		3816.2
(3)
P 		3807
B 6.1
(-3)
A 		50.4
(-2.9)
A 		76
(-2.9)
A 		114.7
(-2.8)
P 		873.7
(-1.4)
P 		3316.3
(3)
P 		3310
C 52.3
(-3)
A 		84
(-2.9)
A 		47.6
(-3)
A 		122.7
(-2.8)
P 		772
(-1.5)
P 		3010.4
(3)
P 		2963
avr 22.5
(-3) 		60.8
(-2.9) 		61.5
(-2.9) 		101.6
(-2.9) 		835.7
(-1.5) 		3381
(3) 		3359
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1213
1 2 3 4 range
A 13.3
(-3)
A 		12.8
(-3)
A 		13
(-3)
A 		862.8
(3)
P 		850
B 22.2
(-2.9)
A 		15.7
(-3)
A 		5.8
(-3)
A 		1219.1
(3)
P 		1213
C 28
(-2.9)
A 		20.2
(-3)
A 		14.7
(-3)
A 		868
(3)
P 		853
avr 21.2
(-2.9) 		16.2
(-3) 		11.2
(-3) 		983.3
(3) 		972

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1235
1 2 3 4 5 6 range
A 1.7
(-3)
A 		17.1
(-2.9)
A 		13.3
(-2.9)
A 		17.1
(-2.9)
P 		259.9
(-1.3)
P 		929.1
(3)
P 		927
B 2.1
(-3)
A 		16.8
(-2.9)
A 		18.4
(-2.9)
A 		30.4
(-2.9)
P 		355
(-1.3)
P 		1236.6
(3)
P 		1235
C 19.4
(-3)
A 		25.6
(-2.9)
A 		12.1
(-3)
A 		24.7
(-2.9)
P 		249.8
(-1.6)
P 		1007.7
(3)
P 		996
avr 7.7
(-3) 		19.8
(-2.9) 		14.6
(-3) 		24.1
(-2.9) 		288.2
(-1.4) 		1057.8
(3) 		1050
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187701_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4562
Sequence Type Exemplar sequence
Representative Public ID T25D10.3 NCBI
Target Description T25D10.3 /REP_DB=WormBase Gene ID /WP=CE02060 /TR=SW:Q10018 /GB=AAC46590.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062890(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062890 NCBI Caenorhabditis elegans SaPosin-like Protein family member (spp-11) (spp-11) mRNA, complete cds. 11/11 None
GENEFINDER00000021467 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6405387:6407289:-1 11/11 None
T25D10.3 ENSEMBL cdna:known chromosome:CEL140:II:6405387:6407289:-1 gene:T25D10.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000021461 3/11 Cross Hyb Matching Probes None
SNAP00000021462 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6405383-6407286(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol spp-11
Ensembl T25D10.3
Entrez Gene 174063 Entrez gene
SwissProt Q10018 EMBL-EBI
WormBase CE30192 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.8961.1.S1_AT similar to Periaxin cfa
HG-U133_PLUS_2:220024_S_AT periaxin hs
HG-U133A:220024_S_AT periaxin hs
HG-U133A_2:220024_S_AT periaxin hs
HU35KSUBA:HUM_ALU_AT periaxin hs
HU35KSUBB:HUM_ALU_AT periaxin hs
HU35KSUBC:HUM_ALU_AT periaxin hs
HU35KSUBD:HUM_ALU_AT periaxin hs
HUGENEFL:HUM_ALU_AT periaxin hs
HC-G110:HUM_ALU_AT periaxin hs
U133_X3P:G13491171_3P_A_AT periaxin hs
HG-U95D:89201_AT periaxin hs
HG-U133_PLUS_2:222255_AT periaxin hs
HG-U133A_2:222255_AT periaxin hs
HG-U133A:222255_AT periaxin hs
MOE430A:1423292_A_AT periaxin mm
MOUSE430A_2:1423292_A_AT periaxin mm
MOUSE430_2:1423292_A_AT periaxin mm
MG-U74AV2:102214_AT periaxin mm
MG-U74CV2:169669_F_AT periaxin mm
RG-U34A:Z29649_AT hypothetical gene supported by NM_023976 rn
RAE230A:1368090_AT hypothetical gene supported by NM_023976 rn
RAT230_2:1368090_AT hypothetical gene supported by NM_023976 rn
Protein Similarities
Method ID Description E-Value
blast NP_495291 SaPosin-like Protein family member (spp-11) [Caenorhabditis elegans] gb|AAC46590.2| Saposin-like protein family protein 11 [Caenorhabditis elegans] sp|Q10018|YSW3_CAEEL Hypothetical protein T25D10.3 in chromosome II precursor 1.0E-128
blast NP_495291 SaPosin-like Protein family member (spp-11) [Caenorhabditis elegans] gb|AAC46590.2| Saposin-like protein family protein 11 [Caenorhabditis elegans] sp|Q10018|YSW3_CAEEL Hypothetical protein T25D10.3 in chromosome II precursor 1.0E-126
blast T16940 hypothetical protein T25D10.3 - Caenorhabditis elegans 1.0E-121
blast T16940 hypothetical protein T25D10.3 - Caenorhabditis elegans 1.0E-120
Sequence
Target Sequence 
>C. ELEGANS:187701_AT
taattcctattaccttaccaacaattaaaattccagaaatagtcccaactaaactaccca
ctgtagaagtacccgatactataccaaaaactcttccaactactaagatccccgatattg
ttccaattacctcgccaacagtaaaaattcctcagataatccctactatcaaaattccgg
atattattcctaaaaatctgtcaactttggggccaattaaacttccaaccattaagcttc
caacgggaaatatggtttgcgatatttgcgagaaagtgatcggtgttcttaccacaagac
ttcttgaaattatacaaaagtttagagtggaagctgataaattccttacgaaactctgca
cgtctttaacaagcaatccgaaaactctcacagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAATTCCTATTACCTTACCAACAAT 68 635 632 Antisense
CTACCCACTGTAGAAGTACCCGATA 109 227 685 Antisense
TATACCAAAAACTCTTCCAACTACT 652 651 711 Antisense
TTCCAACTACTAAGATCCCCGATAT 167 699 725 Antisense
GATATTGTTCCAATTACCTCGCCAA 440 427 745 Antisense
AAATTCCTCAGATAATCCCTACTAT 632 115 776 Antisense
AAATCTGTCAACTTTGGGGCCAATT 48 119 825 Antisense
ACTTCCAACCATTAAGCTTCCAACG 29 99 852 Antisense
GTGATCGGTGTTCTTACCACAAGAC 520 483 907 Antisense
GAAACTCTGCACGTCTTTAACAAGC 399 357 981 Antisense
CAAGCAATCCGAAAACTCTCACAGT 432 185 1001 Antisense
Sequence Source Affymetrix Proprietary Database