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[187700_at] E-1 enzyme

Description F58H1.3 /REP_DB=WormBase Gene ID /WP=CE06027 /GB=CAB00106.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=E-1 enzyme
Cluster 6grp_highest_10_10 > 45      6grp_high1_10_10 > 53      4grp_highest_8_8 > 56      4grp_high_8_8 > 36      6grp_highest_nonorm > 62      6grp_total > 54      4grp_total > 36      6grp_high1_sub > 03      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 109
1 2 3 4 range
A 314.6
(-1.3)
P 		333.6
(0.2)
P 		369.3
(3)
P 		293.7
(-3)
P 		76
B 352.3
(-0.3)
P 		402.6
(3)
P 		310.6
(-3)
P 		347.8
(-0.6)
P 		92
C 386.6
(3)
P 		354.9
(0.8)
P 		299.3
(-3)
P 		383.3
(2.8)
P 		87
avr 351.2
(1) 		363.7
(3) 		326.4
(-3) 		341.6
(-0.6) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 389
1 2 3 4 5 6 range
A 331.3
(-3)
P 		414.2
(-1.5)
P 		667.4
(3)
P 		496.2
(-0.1)
P 		434.1
(-1.2)
P 		410.4
(-1.6)
P 		336
B 359.3
(-2.9)
P 		606.3
(2.5)
P 		627.8
(3)
P 		441
(-1.1)
P 		356.4
(-3)
P 		479
(-0.3)
P 		271
C 278.9
(-3)
P 		575.2
(3)
P 		454.8
(0.6)
P 		499.4
(1.5)
P 		375.6
(-1)
P 		524.6
(2)
P 		296
avr 323.2
(-3) 		531.9
(1.8) 		583.3
(3) 		478.9
(0.6) 		388.7
(-1.5) 		471.3
(0.4) 		260
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 85
1 2 3 4 range
A 114.6
(3)
P 		72.4
(-0.5)
P 		96.6
(1.5)
P 		43
(-3)
P 		72
B 112.4
(3)
P 		99.4
(1.1)
P 		72
(-3)
P 		74.1
(-2.7)
P 		40
C 128
(3)
P 		102
(0.5)
P 		104.3
(0.8)
P 		64.6
(-3)
P 		63
avr 118.3
(3) 		91.3
(0.2) 		91
(0.2) 		60.6
(-3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 141
1 2 3 4 5 6 range
A 61.1
(-3)
P 		147.3
(3)
P 		145.4
(2.9)
P 		125.8
(1.5)
P 		131
(1.9)
P 		99.9
(-0.3)
P 		86
B 125
(-2.4)
P 		201.8
(3)
P 		152.5
(-0.5)
P 		116.8
(-3)
P 		144.8
(-1)
P 		178.6
(1.4)
P 		85
C 103.4
(-2.8)
P 		175.5
(3)
P 		115.8
(-1.8)
P 		100.4
(-3)
P 		121.5
(-1.3)
P 		175.6
(3)
P 		75
avr 96.5
(-3) 		174.9
(3) 		137.9
(0.2) 		114.3
(-1.6) 		132.4
(-0.3) 		151.4
(1.2) 		78
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187700_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14570
Sequence Type Exemplar sequence
Representative Public ID F58H1.3 NCBI
Target Description F58H1.3 /REP_DB=WormBase Gene ID /WP=CE06027 /GB=CAB00106.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=E-1 enzyme
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073596(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073596 NCBI Caenorhabditis elegans F58H1.3 (F58H1.3) mRNA, complete cds. 11/11 None
SNAP00000006509 ENSEMBL cdna:SNAP chromosome:CEL140:V:11936566:11939094:1 10/11 None
GENEFINDER00000006515 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11936566:11939094:1 11/11 None
F58H1.3 ENSEMBL cdna:known chromosome:CEL140:V:11936566:11939094:1 gene:F58H1.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11936565-11939094(+) 100.0 100.0
Public Domain and Genome References
Gene Title E-1 enzyme
Ensembl F58H1.3
Entrez Gene 179634 Entrez gene
SwissProt Q21012 EMBL-EBI
WormBase CE06027 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:248234_AT haloacid dehalogenase-like hydrolase family protein at
CANINE:1585113_AT similar to E-1 enzyme cfa
CANINE_2:CFA.6825.1.A1_AT similar to E-1 enzyme cfa
CANINE_2:CFAAFFX.14162.1.S1_AT similar to E-1 enzyme cfa
DROSOPHILA_2:1630073_AT dm
DROSGENOME1:149316_AT dm
CHICKEN:GGA.5415.2.S1_A_AT similar to E-1 enzyme gga
HG-U133_PLUS_2:217956_S_AT E-1 enzyme hs
HG-U133A:217956_S_AT E-1 enzyme hs
HG-FOCUS:217956_S_AT E-1 enzyme hs
HG-U133A_2:217956_S_AT E-1 enzyme hs
HU35KSUBA:AA285293_AT E-1 enzyme hs
HU35KSUBB:RC_W04517_AT E-1 enzyme hs
U133_X3P:G10864016_3P_A_AT E-1 enzyme hs
HG-U95C:49566_AT E-1 enzyme hs
HG-U95B:52331_S_AT E-1 enzyme hs
HG-U133_PLUS_2:228682_AT E-1 enzyme hs
HG-U133B:228682_AT E-1 enzyme hs
U133_X3P:HS.18442.2.A1_3P_AT E-1 enzyme hs
MG-U74AV2:160067_AT RIKEN cDNA 2310057D15 gene mm
MG-U74CV2:167168_F_AT RIKEN cDNA 2310057D15 gene mm
MOE430A:1423705_AT RIKEN cDNA 2310057D15 gene mm
MOUSE430A_2:1423705_AT RIKEN cDNA 2310057D15 gene mm
MOUSE430_2:1423705_AT RIKEN cDNA 2310057D15 gene mm
MOE430A:1437327_X_AT RIKEN cDNA 2310057D15 gene mm
MOUSE430A_2:1437327_X_AT RIKEN cDNA 2310057D15 gene mm
MOUSE430_2:1437327_X_AT RIKEN cDNA 2310057D15 gene mm
MG-U74BV2:116529_AT RIKEN cDNA 2310057D15 gene (2310057D15Rik), mRNA mm
MG-U74CV2:169902_AT RIKEN cDNA 2310057D15 gene (2310057D15Rik), mRNA mm
MU19KSUBA:TC15710_AT RIKEN cDNA 2310057D15 gene (2310057D15Rik), mRNA mm
MU19KSUBA:TC20608_AT RIKEN cDNA 2310057D15 gene (2310057D15Rik), mRNA mm
RG-U34C:RC_AI144631_AT similar to RIKEN cDNA 2310057D15 (predicted) rn
RG-U34C:RC_AA858537_AT similar to RIKEN cDNA 2310057D15 (predicted) rn
RAE230A:1372488_AT similar to RIKEN cDNA 2310057D15 (predicted) rn
RAT230_2:1372488_AT similar to RIKEN cDNA 2310057D15 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB00106 Hypothetical protein F58H1.3 [Caenorhabditis elegans] ref|NP_505997.1| F58H1.3 [Caenorhabditis elegans] 0.0
blast CAB00106 Hypothetical protein F58H1.3 [Caenorhabditis elegans] ref|NP_505997.1| F58H1.3 [Caenorhabditis elegans] 1.0E-128
blast CAE75352 Hypothetical protein CBG23332 [Caenorhabditis briggsae] 1.0E-111
blast CAE75352 Hypothetical protein CBG23332 [Caenorhabditis briggsae] 1.0E-109
blast CAE75352 Hypothetical protein CBG23332 [Caenorhabditis briggsae] 1.0E-108
blast CAE75354 Hypothetical protein CBG23334 [Caenorhabditis briggsae] 2.0E-70
blast CAE75354 Hypothetical protein CBG23334 [Caenorhabditis briggsae] 3.0E-70
Protein Domains
Database ID Description E-Value
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 1.4E-16
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 3.4E-17
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 2.8E-17
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505997.1 2 348-370,390-412
F58H1.3 2 348-370,390-412
SNAP00000006509 2 251-273,293-315
GENEFINDER00000006515 2 332-354,374-396
Sequence
Target Sequence 
>C. ELEGANS:187700_AT
gacaatattcgaaaagcccaccaaaaagacaacgtcgacgaaaaggaatgggcattgatt
tatagagatgttggagcatcgagaacattttacatgactgctgcttttgttccatccttc
atcgttggctcttcaatttttgctttcgatgtcaattcaaacagtcctggaaatcgattc
gattttgtgcagaagctagtgacggattcagaagaacttggagttctcattgcacttcca
tcagttgcattagcagttgtatttgcctgtttaatccgtgttcagcagttacgactaatt
aggatataccagaataaaaatgacgcggagcagtttatggctatacggtcgaaattcatt
gttaagcagcacaaagagctgttccgacgagatgaaacaactggtttctacttcgcagat
gatcaaactgacggtgtccgagttgcaatgcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAATATTCGAAAAGCCCACCAAA 702 361 967 Antisense
TACATGACTGCTGCTTTTGTTCCAT 111 645 1057 Antisense
TCTTCAATTTTTGCTTTCGATGTCA 243 615 1096 Antisense
GAACTTGGAGTTCTCATTGCACTTC 357 349 1180 Antisense
TTCTCATTGCACTTCCATCAGTTGC 122 691 1190 Antisense
GTATTTGCCTGTTTAATCCGTGTTC 201 453 1225 Antisense
AATCCGTGTTCAGCAGTTACGACTA 211 167 1239 Antisense
GACGCGGAGCAGTTTATGGCTATAC 484 381 1288 Antisense
ACAAAGAGCTGTTCCGACGAGATGA 57 113 1337 Antisense
ACAACTGGTTTCTACTTCGCAGATG 342 113 1363 Antisense
TGACGGTGTCCGAGTTGCAATGCAT 103 571 1395 Antisense
Sequence Source Affymetrix Proprietary Database