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[187697_at]

Description C28C12.3 /REP_DB=WormBase Gene ID /WP=CE04110 /TR=SW:P50437 /GB=AAB37546.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 93      4grp_total > 25      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 68
1 2 3 4 range
A 10.2
(-3)
A 		10.9
(-2.8)
A 		32.9
(2.8)
A 		33.8
(3)
A 		24
B 11.4
(-3)
A 		78.5
(3)
A 		30.3
(-1.3)
A 		56.7
(1.1)
A 		67
C 27
(0.1)
A 		14.9
(-3)
A 		29.6
(0.7)
A 		38.7
(3)
A 		24
avr 16.2
(-3) 		34.8
(1.1) 		30.9
(0.3) 		43.1
(3) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 20.8
(-2.6)
A 		40.3
(-0.3)
A 		20
(-2.7)
A 		32.3
(-1.3)
A 		68.9
(3)
A 		17.3
(-3)
A 		52
B 11.6
(-3)
A 		28.8
(-1.8)
A 		44.9
(-0.7)
A 		29.2
(-1.8)
A 		97.8
(3)
A 		64.3
(0.7)
A 		86
C 12.4
(-3)
A 		59.6
(1.4)
A 		70
(2.4)
A 		13.1
(-2.9)
A 		76.8
(3)
A 		60.1
(1.4)
A 		64
avr 14.9
(-3) 		42.9
(-0.5) 		45
(-0.3) 		24.9
(-2.1) 		81.2
(3) 		47.2
(-0.1) 		66
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 3.7
(-1.7)
A 		2.4
(-3)
A 		8.6
(3)
A 		4.9
(-0.6)
A 		6
B 3.6
(-3)
A 		19.4
(3)
A 		7
(-1.7)
A 		12.1
(0.2)
A 		16
C 8.9
(1.6)
A 		4.3
(-3)
A 		10.3
(3)
A 		6.5
(-0.8)
A 		6
avr 5.4
(-3) 		8.7
(3) 		8.6
(2.8) 		7.8
(1.4) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 37
1 2 3 4 5 6 range
A 3.8
(-3)
A 		14.3
(0.7)
A 		4.4
(-2.8)
A 		8.2
(-1.4)
A 		20.8
(3)
A 		4.2
(-2.9)
A 		17
B 4
(-3)
A 		9.6
(-2.1)
A 		10.9
(-1.8)
A 		7.7
(-2.4)
A 		39.8
(3)
A 		24
(0.4)
A 		36
C 4.6
(-2.5)
A 		18.2
(1.2)
A 		17.8
(1.1)
A 		2.6
(-3)
A 		24.8
(3)
A 		20.1
(1.7)
A 		22
avr 4.1
(-3) 		14
(-0.6) 		11
(-1.3) 		6.2
(-2.5) 		28.5
(3) 		16.1
(-0) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187697_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9233
Sequence Type Exemplar sequence
Representative Public ID C28C12.3 NCBI
Target Description C28C12.3 /REP_DB=WormBase Gene ID /WP=CE04110 /TR=SW:P50437 /GB=AAB37546.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069048(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069048 NCBI Caenorhabditis elegans C28C12.3 (C28C12.3) mRNA, complete cds. 11/11 None
SNAP00000012167 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8490203:8490839:1 11/11 None
GENEFINDER00000012182 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8490203:8490839:1 11/11 None
C28C12.3 ENSEMBL cdna:known chromosome:CEL140:IV:8490203:8490839:1 gene:C28C12.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8490208-8490845(+) 93.54 100.0
Public Domain and Genome References
Gene Title
Ensembl C28C12.3
Entrez Gene 182977 Entrez gene
SwissProt P50437 EMBL-EBI
WormBase CE04110 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37546 Hypothetical protein C28C12.3 [Caenorhabditis elegans] ref|NP_501449.1| C28C12.3 [Caenorhabditis elegans] sp|P50437|YY23_CAEEL Hypothetical protein C28C12.3 in chromosome IV 1.0E-63
blast CAE71549 Hypothetical protein CBG18492 [Caenorhabditis briggsae] 1.0E-34
blast CAE62000 Hypothetical protein CBG06008 [Caenorhabditis briggsae] 6.0E-30
Protein Domains
Database ID Description E-Value
Pfam IPR003677 EMBL-EBI Protein of unknown function, DUF 148 1.1E-60
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501449.1 1 5-24
C28C12.3 1 5-24
SNAP00000012167 1 5-24
GENEFINDER00000012182 1 5-24
Sequence
Target Sequence 
>C. ELEGANS:187697_AT
tggctcaaaacttgctcttctgcaccatcgccttcgtcttcgtagccgtcgccatggcag
gacctggaggtcgtggaggacctcatggtccaggagggcatggcccacgtggcggaccag
gacttccaccattccttgttaatgtcactgcagcaggaagaaaagagtttgaagctgttt
tcaagaatgaaacgttaacaattgctgaagctaatacccaaattgctgctctcgctgaga
aatacggagtcacagcaacctacaatgagtttgtggctaatcgcaccgctctactcgccg
aggttaagaagaatcaaactgcagtgattggaaatcttacagcagtctctgcccaattgg
ccacaatctaccaaaacaaggatcaaactcgtaaagcacaggaagaagctgttgatgctc
ttcgcaaagaacatccagttgaggtgaatgctatcaagttcattcgtgcccagctcggcg
gagagaaggtgcatggaggctcacatggaggactccgtggtgggccaggagggccacgag
atggacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGCTCAAAACTTGCTCTTCTGCAC 155 545 14 Antisense
GTCGCCATGGCAGGACCTGGAGGTC 387 475 61 Antisense
ATTCCTTGTTAATGTCACTGCAGCA 643 7 144 Antisense
CTAATACCCAAATTGCTGCTCTCGC 166 227 224 Antisense
ATACGGAGTCACAGCAACCTACAAT 333 23 255 Antisense
TGAGTTTGTGGCTAATCGCACCGCT 476 567 279 Antisense
ACCGCTCTACTCGCCGAGGTTAAGA 229 83 298 Antisense
GGAAATCTTACAGCAGTCTCTGCCC 392 527 343 Antisense
TGCTATCAAGTTCATTCGTGCCCAG 148 579 462 Antisense
GTGCCCAGCTCGGCGGAGAGAAGGT 500 477 479 Antisense
GGCCAGGAGGGCCACGAGATGGACC 694 541 536 Antisense
Sequence Source Affymetrix Proprietary Database