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[187689_at]

Description T20B12.4 /REP_DB=WormBase Gene ID /WP=CE01410 /TR=SW:P41844 /GB=AAA19057.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 43      4grp_total > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 2
(-2.9)
A 		1.6
(-3)
A 		6
(-1.9)
A 		25
(3)
A 		23
B 6.3
(3)
A 		3.4
(-0.5)
A 		1.3
(-3)
A 		2.5
(-1.6)
A 		5
C 2.7
(-2.8)
A 		4.3
(-2.6)
A 		1
(-3)
A 		49.3
(3)
A 		48
avr 3.7
(-2.8) 		3.1
(-2.9) 		2.8
(-3) 		25.6
(3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 24.7
(3)
A 		2.3
(-2.9)
A 		4.7
(-2.3)
A 		1.9
(-3)
A 		6.3
(-1.8)
A 		2.5
(-2.8)
A 		23
B 2.6
(-2.8)
A 		2.2
(-2.9)
A 		35.2
(3)
A 		1.4
(-3)
A 		20.6
(0.4)
A 		1.4
(-3)
A 		34
C 1.5
(-2.7)
A 		6.1
(1.2)
A 		8.2
(3)
A 		4
(-0.6)
A 		1.2
(-3)
A 		2.2
(-2.1)
A 		7
avr 9.6
(0.3) 		3.5
(-2.4) 		16
(3) 		2.4
(-2.8) 		9.4
(0.2) 		2
(-3) 		14
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 0.7
(-2.5)
A 		0.4
(-3)
A 		1.6
(-0.8)
A 		3.7
(3)
A 		3
B 2
(3)
A 		0.8
(-1.2)
A 		0.3
(-3)
A 		0.5
(-2.3)
A 		2
C 0.9
(-2.6)
A 		1.2
(-2.4)
A 		0.4
(-3)
A 		8.3
(3)
A 		8
avr 1.2
(-2.3) 		0.8
(-3) 		0.8
(-3) 		4.2
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 4.6
(3)
A 		0.8
(-2.6)
A 		1
(-2.3)
A 		0.5
(-3)
A 		1.9
(-1)
A 		0.6
(-2.9)
A 		4
B 0.9
(-2.6)
A 		0.7
(-2.8)
A 		8.6
(3)
A 		0.4
(-3)
A 		8.4
(2.9)
A 		0.5
(-2.9)
A 		8
C 0.6
(-2.3)
A 		1.9
(2.3)
A 		2.1
(3)
A 		0.8
(-1.6)
A 		0.4
(-3)
A 		0.7
(-1.9)
A 		2
avr 2
(-0.5) 		1.1
(-2.1) 		3.9
(3) 		0.6
(-3) 		3.6
(2.5) 		0.6
(-3) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187689_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6873
Sequence Type Exemplar sequence
Representative Public ID T20B12.4 NCBI
Target Description T20B12.4 /REP_DB=WormBase Gene ID /WP=CE01410 /TR=SW:P41844 /GB=AAA19057.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066229(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066229 NCBI Caenorhabditis elegans T20B12.4 (T20B12.4) mRNA, complete cds. 11/11 None
GENEFINDER00000022569 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7365008:7369458:1 11/11 None
T20B12.4 ENSEMBL cdna:known chromosome:CEL140:III:7367630:7369458:1 gene:T20B12.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7367627-7369456(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T20B12.4
Entrez Gene 188631 Entrez gene
SwissProt P41844 EMBL-EBI
WormBase CE01410 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA19057 Hypothetical protein T20B12.4 [Caenorhabditis elegans] ref|NP_498630.1| T20B12.4 [Caenorhabditis elegans] sp|P41844|YO94_CAEEL Hypothetical protein T20B12.4 in chromosome III 0.0
blast NP_504159 Y45G5AM.3 [Caenorhabditis elegans] gb|AAG23365.1| Hypothetical protein Y45G5AM.3 [Caenorhabditis elegans] 1.0E-103
blast NP_504640 T05H4.15a [Caenorhabditis elegans] gb|AAB66020.1| Hypothetical protein T05H4.15a [Caenorhabditis elegans] 1.0E-102
blast NP_504159 Y45G5AM.3 [Caenorhabditis elegans] gb|AAG23365.1| Hypothetical protein Y45G5AM.3 [Caenorhabditis elegans] 3.0E-82
blast NP_504640 T05H4.15a [Caenorhabditis elegans] gb|AAB66020.1| Hypothetical protein T05H4.15a [Caenorhabditis elegans] 8.0E-82
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 3.0E-7
Sequence
Target Sequence 
>C. ELEGANS:187689_AT
gtagtacagtcagaagcttagcgcgttgcaaaacaattcgaattaatgcaacggatattg
ccacgtattcatggtggcttgaaaaatgcccagaaaaattggattatcttgaattgaaaa
ctctttattcgaataaagacacattcacaattccaacggattttctggatttttcacaga
taaagaatgccggaaccttttatttttggtgtcgctctgcattcacagacgagcaatttc
taaatttaaaagcaaaaaaatttggattccattgtgttaacataactgctaatggcatta
atgatttcattaagaaatgggtcaatggacacggagtaaatgaatttcaaaaggctttgc
tatggtctgcgaaattaaataattcttttatgataatgagaggaattgaatttcgttcat
gggatcaaacatttcgagagcaagaagcggaattttgttctgccttcaatgaagaatttc
ttgatggagagaaaatacaagtgtacagtagaattgatccatatgagagtatcactattt
acttttcacctgacacaatttcaattattgcaactgggcatagaatgactcgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTAGTACAGTCAGAAGCTTAGCGCG 551 455 392 Antisense
GCTTAGCGCGTTGCAAAACAATTCG 319 305 407 Antisense
CAACGGATATTGCCACGTATTCATG 558 187 440 Antisense
CTTTTATTTTTGGTGTCGCTCTGCA 566 219 588 Antisense
GTCGCTCTGCATTCACAGACGAGCA 524 473 602 Antisense
AAGGCTTTGCTATGGTCTGCGAAAT 145 161 742 Antisense
GAATTTCGTTCATGGGATCAAACAT 266 325 799 Antisense
AAGCGGAATTTTGTTCTGCCTTCAA 76 151 836 Antisense
GTATCACTATTTACTTTTCACCTGA 9 455 920 Antisense
TACTTTTCACCTGACACAATTTCAA 349 645 931 Antisense
GCAACTGGGCATAGAATGACTCGAA 410 319 961 Antisense
Sequence Source Affymetrix Proprietary Database