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[187685_s_at]

Description F49C12.5B /REP_DB=WormBase Gene ID /WP=CE05895 /TR=SW:Q20593 /GB=CAA92518.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 10      4grp_total > 09      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 95
1 2 3 4 range
A 21.4
(-3)
A 		36.4
(0.4)
A 		23.5
(-2.5)
A 		48.2
(3)
A 		27
B 32.8
(0.7)
A 		8.3
(-3)
A 		25.3
(-0.4)
A 		47.6
(3)
A 		39
C 5.6
(-3)
A 		34.1
(-1.2)
A 		44.1
(-0.6)
A 		100.7
(3)
A 		95
avr 19.9
(-3) 		26.3
(-2.2) 		31
(-1.5) 		65.5
(3) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 475
1 2 3 4 5 6 range
A 8.8
(-3)
A 		435.8
(3)
P 		308.3
(1.2)
P 		240.9
(0.3)
P 		277.3
(0.8)
P 		125.6
(-1.4)
A 		427
B 5.5
(-3)
A 		446.5
(3)
P 		258.6
(0.4)
P 		175.6
(-0.7)
M 		224.9
(-0)
P 		96
(-1.8)
A 		441
C 5.9
(-3)
A 		480.5
(3)
P 		225.1
(-0.2)
P 		223.2
(-0.3)
M 		149.7
(-1.2)
A 		94.1
(-1.9)
A 		475
avr 6.7
(-3) 		454.3
(3) 		264
(0.4) 		213.2
(-0.2) 		217.3
(-0.2) 		105.2
(-1.7) 		448
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 7.8
(2.7)
A 		7.9
(3)
A 		6.1
(-3)
A 		7.1
(0.3)
A 		2
B 10.5
(3)
A 		2.1
(-3)
A 		5.9
(-0.3)
A 		10.2
(2.8)
A 		8
C 1.9
(-3)
A 		9.8
(0.1)
A 		15.4
(2.4)
A 		17
(3)
A 		15
avr 6.7
(-2.9) 		6.6
(-3) 		9.1
(0.1) 		11.4
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 153
1 2 3 4 5 6 range
A 1.6
(-3)
A 		155
(3)
P 		67.1
(-0.4)
P 		61.1
(-0.7)
P 		83.7
(0.2)
P 		30.6
(-1.9)
A 		153
B 1.9
(-3)
A 		148.6
(3)
P 		62.8
(-0.5)
P 		46.5
(-1.2)
M 		91.4
(0.7)
P 		35.8
(-1.6)
A 		147
C 2.2
(-3)
A 		146.6
(3)
P 		57.3
(-0.7)
P 		44.9
(-1.2)
M 		48.4
(-1.1)
A 		31.5
(-1.8)
A 		144
avr 1.9
(-3) 		150.1
(3) 		62.4
(-0.6) 		50.8
(-1) 		74.5
(-0.1) 		32.6
(-1.8) 		148
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187685_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9521
Sequence Type Exemplar sequence
Representative Public ID F49C12.5B NCBI
Target Description F49C12.5B /REP_DB=WormBase Gene ID /WP=CE05895 /TR=SW:Q20593 /GB=CAA92518.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069226(11), NM_069227(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069226 NCBI Caenorhabditis elegans F49C12.5a (F49C12.5) mRNA, complete cds. 11/11 None
NM_069227 NCBI Caenorhabditis elegans F49C12.5b (F49C12.5) mRNA, complete cds. 11/11 None
SNAP00000032693 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9305302:9307142:1 9/11 None
GENEFINDER00000032716 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9304920:9307142:1 11/11 None
F49C12.5a ENSEMBL cdna:known chromosome:CEL140:IV:9304920:9307142:1 gene:F49C12.5 11/11 None
F49C12.5b ENSEMBL cdna:known chromosome:CEL140:IV:9304920:9307142:1 gene:F49C12.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9304925-9307148(+) 100.0 100.0
Public Domain and Genome References
Ensembl F49C12.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92519 Hypothetical protein F49C12.5a [Caenorhabditis elegans] ref|NP_501627.1| F49C12.5a [Caenorhabditis elegans] sp|Q20593|YE25_CAEEL Hypothetical protein F49C12.5 in chromosome IV 0.0
blast CAA92518 Hypothetical protein F49C12.5b [Caenorhabditis elegans] ref|NP_501628.1| F49C12.5b [Caenorhabditis elegans] 0.0
blast CAE64028 Hypothetical protein CBG08623 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR008166 EMBL-EBI Protein of unknown function DUF23 1.0E-126
Pfam IPR008166 EMBL-EBI Protein of unknown function DUF23 1.0E-126
Pfam IPR008166 EMBL-EBI Protein of unknown function DUF23 4.7E-83
Pfam IPR008166 EMBL-EBI Protein of unknown function DUF23 3.7E-58
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501627.1 1 12-29
NP_501628.1 1 12-29
F49C12.5a 1 12-29
F49C12.5b 1 12-29
GENEFINDER00000032716 1 12-29
Sequence
Target Sequence 
>C. ELEGANS:187685_S_AT
acgtcgctgcgacaagatatgggtcaaaattttctctcaaaaatatgttcaatagtatgg
aatgccaaaacaatagagaaacagggaagattgtggtgaatccaaaacatctcaacttta
cctggatccattatccgccaatttcgccggaagaacttgcaaaacatgaagtaacagaaa
atgtgattactcatctcaagactatagtttgggttgatgaggaaccagacggggaaaacc
aaaaaataattgagccattgtactatgacaattcgaccgatacaatactttctcgacaac
aaataaaggaacttgagacagatcttgagaatatgatgatgaaacccaatatacagaaga
tctttgcacaactacctgacattcattattatacagatttagttgtaaattgctataacg
atcgatattataagtaccattactccggtcgaatctcagaaattacctgtcctggtccac
aattat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGTCGCTGCGACAAGATATGGGTC 284 93 830 Antisense
GATATGGGTCAAAATTTTCTCTCAA 194 423 845 Antisense
ACATCTCAACTTTACCTGGATCCAT 110 107 936 Antisense
TGGATCCATTATCCGCCAATTTCGC 151 551 952 Antisense
GCCAATTTCGCCGGAAGAACTTGCA 49 279 966 Antisense
AATGTGATTACTCATCTCAAGACTA 205 163 1009 Antisense
GTACTATGACAATTCGACCGATACA 91 461 1089 Antisense
GACCGATACAATACTTTCTCGACAA 291 383 1104 Antisense
ATCTTTGCACAACTACCTGACATTC 414 31 1189 Antisense
AAGTACCATTACTCCGGTCGAATCT 323 157 1261 Antisense
ATTACCTGTCCTGGTCCACAATTAT 57 13 1291 Antisense
Sequence Source Affymetrix Proprietary Database