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[187681_at]

Description T25D10.4 /REP_DB=WormBase Gene ID /WP=CE02061 /TR=SW:Q10019 /GB=AAC46591.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 09      6grp_high1_10_10 > 90      4grp_highest_8_8 > 14      4grp_high_8_8 > 15      6grp_highest_nonorm > 83      6grp_total > 0      4grp_total > 08      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1778
1 2 3 4 range
A 246.5
(-3)
P 		377.4
(-2.3)
P 		735.5
(-0.4)
P 		1384.3
(3)
P 		1138
B 216.9
(-3)
P 		464.9
(-2.2)
P 		480.2
(-2.1)
P 		1995.1
(3)
P 		1778
C 246.1
(-3)
P 		489.8
(-1.7)
P 		598.4
(-1.1)
P 		1331.9
(3)
P 		1086
avr 236.5
(-3) 		444
(-2.1) 		604.7
(-1.3) 		1570.4
(3) 		1334

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 500
1 2 3 4 5 6 range
A 283.8
(-3)
P 		501.9
(0.7)
P 		328
(-2.2)
P 		369.9
(-1.5)
P 		635.8
(3)
P 		437.5
(-0.4)
P 		352
B 261
(-3)
P 		718.8
(2.7)
P 		270.6
(-2.9)
P 		524.8
(0.3)
P 		745.7
(3)
P 		531.5
(0.3)
P 		485
C 245.7
(-3)
P 		582.5
(3)
P 		373
(-0.7)
P 		516.7
(1.8)
P 		461.9
(0.9)
P 		548.8
(2.4)
P 		337
avr 263.5
(-3) 		601.1
(2.8) 		323.9
(-2) 		470.5
(0.5) 		614.5
(3) 		505.9
(1.1) 		351
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 356
1 2 3 4 range
A 89.7
(-2.6)
P 		81.9
(-3)
P 		192.3
(2.5)
P 		202.6
(3)
P 		121
B 69.2
(-3)
P 		114.8
(-2.2)
P 		111.3
(-2.3)
P 		425.2
(3)
P 		356
C 81.5
(-3)
P 		140.8
(-0.5)
P 		208.6
(2.3)
P 		224.6
(3)
P 		143
avr 80.1
(-3) 		112.5
(-2) 		170.7
(-0.3) 		284.1
(3) 		204

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 251
1 2 3 4 5 6 range
A 52.3
(-3)
P 		178.5
(2.4)
P 		71.4
(-2.2)
P 		93.8
(-1.2)
P 		191.9
(3)
P 		106.5
(-0.7)
P 		140
B 90.8
(-2.4)
P 		239.3
(1.4)
P 		65.7
(-3)
P 		139
(-1.1)
P 		303
(3)
P 		198.2
(0.4)
P 		237
C 91.1
(-3)
P 		177.7
(2.6)
P 		94.9
(-2.8)
P 		103.9
(-2.2)
P 		149.5
(0.8)
P 		183.7
(3)
P 		93
avr 78.1
(-3) 		198.5
(2.3) 		77.3
(-3) 		112.2
(-1.5) 		214.8
(3) 		162.8
(0.7) 		138
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187681_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4753
Sequence Type Exemplar sequence
Representative Public ID T25D10.4 NCBI
Target Description T25D10.4 /REP_DB=WormBase Gene ID /WP=CE02061 /TR=SW:Q10019 /GB=AAC46591.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062891(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062891 NCBI Caenorhabditis elegans T25D10.4 (T25D10.4) mRNA, complete cds. 11/11 None
SNAP00000021462 ENSEMBL cdna:SNAP chromosome:CEL140:II:6405990:6408451:-1 10/11 None
GENEFINDER00000021464 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6407740:6410221:-1 11/11 None
T25D10.4 ENSEMBL cdna:known chromosome:CEL140:II:6407740:6410221:-1 gene:T25D10.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000021463 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6407736-6410218(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T25D10.4
Entrez Gene 174064 Entrez gene
SwissProt Q10019 EMBL-EBI
WormBase CE02061 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_495292 T25D10.4 [Caenorhabditis elegans] gb|AAC46591.1| Hypothetical protein T25D10.4 [Caenorhabditis elegans] sp|Q10019|YSW4_CAEEL Hypothetical protein T25D10.4 in chromosome II 3.0E-94
blast CAE59313 Hypothetical protein CBG02651 [Caenorhabditis briggsae] 1.0E-84
blast NP_495292 T25D10.4 [Caenorhabditis elegans] gb|AAC46591.1| Hypothetical protein T25D10.4 [Caenorhabditis elegans] sp|Q10019|YSW4_CAEEL Hypothetical protein T25D10.4 in chromosome II 2.0E-64
blast CAE59313 Hypothetical protein CBG02651 [Caenorhabditis briggsae] 5.0E-57
blast NP_495291 SaPosin-like Protein family member (spp-11) [Caenorhabditis elegans] gb|AAC46590.2| Saposin-like protein family protein 11 [Caenorhabditis elegans] sp|Q10018|YSW3_CAEEL Hypothetical protein T25D10.3 in chromosome II precursor 7.0E-19
Sequence
Target Sequence 
>C. ELEGANS:187681_AT
ttccactttcgacatgatccgcgagaagatgttctctttgatggagaacgacatgacgct
gctgaagcaactccttcaactcggcgatcaaatcagcgagatcaagaaagaacgattgag
aaggacaatgtcgcaaaacagtctggaatacgatgaagaagatgagaaagaggacaaatt
cgattctggattctctgcttcgatgagtgcagtcaccaacttgtacgtggatgacgaacg
cccacaattcttttcccgtcaaaattcggttcttcgtattccgattcccccgagaagcag
caatcgatttggaccgagaagggttatcagaagaccttcggatattttgccacgacaaca
gacgaataatatcaggactcttcatgtcaacagtgatgactcagattctagctcttcagg
aagcaagactcactcgccaagcagttccgtatacaatgcttctacactaattcttccatc
aaaaactacaaagaatcgctcctcgaattcttcaatcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCCACTTTCGACATGATCCGCGAG 575 697 174 Antisense
ATGACGCTGCTGAAGCAACTCCTTC 392 45 226 Antisense
AACTCCTTCAACTCGGCGATCAAAT 69 141 242 Antisense
CGATTCTGGATTCTCTGCTTCGATG 670 247 354 Antisense
GAGTGCAGTCACCAACTTGTACGTG 135 401 378 Antisense
ATTCCCCCGAGAAGCAGCAATCGAT 328 9 457 Antisense
GATGACTCAGATTCTAGCTCTTCAG 96 413 568 Antisense
TCAGGAAGCAAGACTCACTCGCCAA 409 623 589 Antisense
CTCGCCAAGCAGTTCCGTATACAAT 459 233 606 Antisense
TGCTTCTACACTAATTCTTCCATCA 601 579 630 Antisense
ATCGCTCCTCGAATTCTTCAATCGA 419 37 668 Antisense
Sequence Source Affymetrix Proprietary Database