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[187667_at]

Description T20B12.9 /REP_DB=WormBase Gene ID /WP=CE01415 /TR=SW:P41849 /GB=AAA19062.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 07      4grp_total > 31      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 151
1 2 3 4 range
A 166
(3)
A 		15
(-3)
A 		34.2
(-2.2)
A 		93.9
(0.1)
A 		151
B 103.4
(3)
A 		43.4
(-1.6)
A 		24.7
(-3)
A 		78.9
(1.1)
A 		79
C 98.8
(3)
A 		57.8
(-1)
A 		62.5
(-0.5)
A 		37
(-3)
A 		62
avr 122.7
(3) 		38.7
(-3) 		40.5
(-2.9) 		69.9
(-0.8) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 128
1 2 3 4 5 6 range
A 19.4
(-0.5)
A 		35.2
(1.8)
A 		43.1
(3)
A 		2.8
(-3)
A 		4.7
(-2.7)
A 		5.4
(-2.6)
A 		40
B 130.4
(3)
A 		59.8
(-0.3)
A 		3.9
(-3)
A 		8.3
(-2.8)
A 		3
(-3)
A 		3.8
(-3)
A 		127
C 77.7
(3)
A 		13.4
(-2.2)
A 		6.5
(-2.7)
A 		10.3
(-2.4)
A 		4.5
(-2.9)
A 		2.9
(-3)
A 		75
avr 75.8
(3) 		36.1
(-0.3) 		17.8
(-1.8) 		7.1
(-2.7) 		4.1
(-3) 		4
(-3) 		72
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 57
1 2 3 4 range
A 60.5
(3)
A 		3.3
(-3)
A 		8.9
(-2.4)
A 		13.7
(-1.9)
A 		57
B 33
(3)
A 		10.7
(-1.9)
A 		5.7
(-3)
A 		16.8
(-0.6)
A 		27
C 32.7
(3)
A 		16.6
(-0.6)
A 		21.8
(0.5)
A 		6.2
(-3)
A 		27
avr 42.1
(3) 		10.2
(-3) 		12.1
(-2.6) 		12.2
(-2.6) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 45
1 2 3 4 5 6 range
A 3.6
(-1.5)
A 		12.5
(3)
A 		9.4
(1.4)
A 		0.7
(-3)
A 		1.4
(-2.6)
A 		1.3
(-2.7)
A 		12
B 45.4
(3)
A 		19.9
(-0.4)
A 		0.9
(-3)
A 		2.2
(-2.8)
A 		1.2
(-3)
A 		1.4
(-2.9)
A 		45
C 28.8
(3)
A 		4.1
(-2.3)
A 		1.7
(-2.8)
A 		2.1
(-2.8)
A 		1.5
(-2.9)
A 		1
(-3)
A 		28
avr 25.9
(3) 		12.2
(-0.3) 		4
(-2.3) 		1.7
(-2.9) 		1.4
(-3) 		1.2
(-3) 		25
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187667_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7579
Sequence Type Exemplar sequence
Representative Public ID T20B12.9 NCBI
Target Description T20B12.9 /REP_DB=WormBase Gene ID /WP=CE01415 /TR=SW:P41849 /GB=AAA19062.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066236(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066236 NCBI Caenorhabditis elegans T20B12.9 (T20B12.9) mRNA, complete cds. 11/11 None
SNAP00000022563 ENSEMBL cdna:SNAP chromosome:CEL140:III:7388269:7395304:-1 10/11 None
GENEFINDER00000022566 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7391678:7394840:-1 10/11 None
T20B12.9 ENSEMBL cdna:known chromosome:CEL140:III:7391605:7395203:-1 gene:T20B12.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7391520-7395201(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T20B12.9
Entrez Gene 188633 Entrez gene
SwissProt P41849 EMBL-EBI
WormBase CE32728 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
6811 ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
45211 postsynaptic membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4890 GABA-A receptor activity inferred from electronic annotation QuickGO AmiGO
5230 extracellular ligand-gated ion channel activity inferred from electronic annotation QuickGO AmiGO
30594 neurotransmitter receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA19062 Hypothetical protein T20B12.9 [Caenorhabditis elegans] ref|NP_498637.2| T20B12.9 [Caenorhabditis elegans] sp|P41849|YO99_CAEEL Hypothetical glycine receptor-like protein T20B12.9 in chromosome III 0.0
blast CAE64437 Hypothetical protein CBG09141 [Caenorhabditis briggsae] 0.0
blast CAE72047 Hypothetical protein CBG19129 [Caenorhabditis briggsae] 1.0E-86
Protein Domains
Database ID Description E-Value
Pfam IPR006029 EMBL-EBI Neurotransmitter-gated ion-channel transmembrane region 3.0E-12
Pfam IPR006029 EMBL-EBI Neurotransmitter-gated ion-channel transmembrane region 6.1E-5
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 3.2E-64
Pfam IPR006029 EMBL-EBI Neurotransmitter-gated ion-channel transmembrane region 3.0E-12
Pfam IPR006029 EMBL-EBI Neurotransmitter-gated ion-channel transmembrane region 4.3E-7
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 7.4E-68
Pfam IPR006029 EMBL-EBI Neurotransmitter-gated ion-channel transmembrane region 3.0E-12
Pfam IPR006029 EMBL-EBI Neurotransmitter-gated ion-channel transmembrane region 4.3E-7
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 7.4E-68
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498637.2 4 223-245,250-272,287-309,448-470
T20B12.9 4 223-245,250-272,287-309,448-470
SNAP00000022563 4 229-251,256-278,293-315,463-494
GENEFINDER00000022566 4 211-233,238-260,275-297,436-458
Sequence
Target Sequence 
>C. ELEGANS:187667_AT
ggaggtgttggaggccttctgagaaaacgaattccagtgttggctcaattaaaagcagct
gctacagattcgaattccggtgcggcgactgcaatgacgacggcgattcagccgccaaac
actaatttgaattcaattacaaattcggataatagtaaactagttgctaacaattttacc
tcgatcgagcacgaaacgtatgcctacgagaagaaacgtggtttttctcattgttttcag
cgtttcgtgtatgcaatttgtccgcctgatcgagattggacaattacgagtgtacaagtg
gatcggtgttccatgattatgtttccactgtcttttcttatttttaacgttatactggtc
aatatatttcatgaaaatggatcgtcccatgtaaattcatcacattcccttttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGGTGTTGGAGGCCTTCTGAGAA 309 517 1048 Antisense
GCTACAGATTCGAATTCCGGTGCGG 617 301 1108 Antisense
AATTCCGGTGCGGCGACTGCAATGA 282 177 1120 Antisense
CGACTGCAATGACGACGGCGATTCA 65 251 1133 Antisense
GATTCAGCCGCCAAACACTAATTTG 96 431 1152 Antisense
GTTGCTAACAATTTTACCTCGATCG 632 439 1210 Antisense
GATCGAGCACGAAACGTATGCCTAC 440 417 1230 Antisense
TTCTCATTGTTTTCAGCGTTTCGTG 517 691 1272 Antisense
TCGTGTATGCAATTTGTCCGCCTGA 121 605 1292 Antisense
TATGTTTCCACTGTCTTTTCTTATT 187 657 1365 Antisense
TGTAAATTCATCACATTCCCTTTTC 504 559 1437 Antisense
Sequence Source Affymetrix Proprietary Database